Application of random amplified polymorphic DNA and inter‐simple sequence repeat markers in the genus Crataegus

Abstract

Hawthorn (Crataegus spp.) has a long history as an ornamental and a source of medicine. We report the use of random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers to determine genetic relationships in the genus Crataegus. Twenty-eight accessions, including eight species (Crataegus pinnatifida, Crataegus bretschneideri, Crataegus maximowiczii, Crataegus kansuensis, Crataegus altaica, Crataegus songarica, Crataegus dahurica and Crataegus sanguinea) and two botanical varieties (C. pinnatifida var. major and C. maximowiczii var. ninganensis) were analysed. Twelve RAPD primers reproducibly and strongly amplified 128 fragments of which 116 were polymorphic; similarly, 13 ISSR primers generated 127 products of which 119 were polymorphic. Dendrograms based on unweighted pair group method with arithmetic average analysis were constructed from both the RAPD and the ISSR data. Similarity coefficient based on RAPD and ISSR markers ranged from 0.22 to 0.98 and 0.23 to 0.98, respectively. The range in similarity coefficient indicated that the genus has a high level of genetic diversity. The Mantel test on the similarity matrices produced by RAPD and ISSR markers gave r = 0.86, showing high correlation between RAPD and ISSR markers in their ability to detect genetic relationships between Crataegus accessions. RAPD and ISSR appear to be reliable methods for the analysis of genetic relationships among hawthorns.

Evaluation of Genetic Diversity in Chlorophytum borivilianum (Santp. and Fernan.) Using Molecular Markers: An Endangered Medicinal Plant

“…The coeicient correlation value, r = 0.7591, 0.7263 and 0.7638 for RAPD, ISSR and combined RAPD and ISSR analyses, respectively. This result is corroborated with the earlier report of Dai et al [55].…”

Genetic Relationships Among Some Hawthorn (Crataegus spp.) Species and Genotypes

“…One characterized populations of C. monogyna by RAPD (Ferrazzini et al 2008). Dai et al (2008) characterized 28 hawthorn accessions using RAPD and ISSR. Albarouki and Peterson (2007) also characterized hawthorn accessions by plastid DNA sequences (trnL-trnF, psbAtrnH).…”

Classification and phylogenetic analysis of Chinese hawthorn assessed by plant and pollen morphology

“…Fineschi et al (2005) reviewed the number of Crataegus species in different regions described by several authors and observed that the proposed number of species varied greatly. Including numerous hybrids and variants, the genus Crataegus has been suggested to contain between 140 and 200 species (Lo, 2008;Dai et al, 2007Dai et al, , 2009Dai et al, , 2013. According to Judd et al (1999), the genus Crataegus comprises 265 species.…”

“…It is also used as an alternative therapy for various cardiovascular conditions, because of its flavonoid content (Rigelsky and Sweet, 2002;Kao et al, 2005). Also with high amount of flavonoid, vitamin C, glycoside, anthocynaidin, and other components, the fruits could be used for fresh consumption (eaten raw) or processed in the form of jam, jelly, syrup, juice, preserves, and preserved slices (Dai et al, 2007(Dai et al, , 2009(Dai et al, , 2013.…”

“…major N.E.Br.) originated in northern China and is represented by more than 100 cultivars (Dai et al, 2007(Dai et al, , 2009). At about A.D. 1000, the cultivation of hawthorn started.…”