Evaluation of Genetic Diversity in Chlorophytum borivilianum (Santp. and Fernan.) Using Molecular Markers: An Endangered Medicinal Plant

Abstract

Chlorophytum borivilianum is a traditional medicinal plant distributed throughout the tropics and subtropics. In the present investigation, RAPD and ISSR analyses were used to assess the genetic diversity among 21 accessions collected from diferent geographical regions of India using 20 RAPD primers and 6 ISSR primers. RAPD and ISSR primers revealed 92.26% and 82.76% polymorphism, respectively. Similarity in coeicient values ranged from 0.321 to 0.707 for RAPD and 0.363 to 0.846 for ISSR markers. The dendrogram developed by RAPD and ISSR marker-based analysis grouped the 21 accessions into diferent clusters. Mantel test employed for detection of goodness of it established the cophenetic correlation value for both the primer systems and it was observed to be signiicant. Clustering of accessions within groups was also similar based on RAPD-and ISSR-derived dendrograms. In our study, both marker systems were similar except for the percentage polymorphism which was found to be greater using RAPD, thus indicating the greater efectiveness of RAPD primers for estimating genetic variation of C. borivilianum.

Population genetics of Mesembryanthemum crystallinum in Egypt

“…Mesembryanthemum sp. (61.78% in Egypt; Soliman et al 2014), Chlorophytum borivilianum (92.26% in India; Samantaray and Ngangkham 2017), Salvia officinalis (80%; Liber et al 2014), Lathyrus sativus (20.6% in Germany to 60.3% in Poland; Nosrati et al 2012) and Pterolobium hexapetalum (80 % in India; Sharmila et al 2019). In the same manner, M. crystallinum value was higher than the fourteen Sesuvium portulacastrum L. (Aizoaceae) clones collected from Indian which 70 RAPD primers revealed 749 detectable bands of which 294 were polymorphic with 39.25% polymorphism (Lokhande et al 2009).…”