Application of RAPD and ISSR markers to analyse molecular relationships in Grevillea (Proteaceae)

Abstract

The potential of RAPD and ISSR markers to construct molecular relationships of Grevillea was evaluated with 23 RAPD and 12 ISSR primers. The 16 genotypes representing 12 species and 3 subspecies of Grevillea were sampled from the collection of the Mt Anann Botanic Garden, NSW. RAPD and ISSR assays generated a total of 401 RAPD and 280 ISSR fragments. High frequencies of polymorphisms, 99.39% for RAPD and 99.51% for ISSR, were detected by both markers. Three statistical approaches were employed to construct phylogenetic relationships from combined RAPD and ISSR data. Cluster analysis by the unweighted pair group method (UPGMA) of Jaccard's similarity and Neighbour-Joining analysis of total character difference generated dendograms with similar topology. Parsimony analysis also generated a tree that was in broad agreement with the two dendograms. The phylogenetic trees divided the Grevillea species studied into three groups. Group A consisted of G. buxifolia subsp. buxifolia, G. phylicoides and G. sphacelata. In group B, G. mucronulata was grouped together with G. montana, while G. diffusa, G. humilis, G. linearifolia, G. molyneuxii, G. oldei, G. sericea and G. speciosa formed group C. This molecular result was comparable to groupings suggested by a previous author (Makinson 2000) based on morphological characteristics. However, in contrast to the morphological taxonomy, molecular phylogeny suggests that G. oldei and G. speciosa belong to the same subgroup sensu Makinson (2000), whereas G. linearifolia and G. molyneuxii should not be placed in their originally suggested subgroups sensu Makinson (2000). The present study is the first published report on molecular relationships of Grevillea and can be considered as an initial point for further research on the genetic relationships and evolution of Grevillea.

Genetic Diversity in Aechmea fulgens (Bromeliaceae: Bromelioideae) Revealed by Molecular Markers

“…In these studies the number of fragments ranged from 8-23 (UCB 808), 7-10 (UCB 842) and 8-24 (UCB 890). Pharmawati et al (2004) obtained fragments for the oligonucletides UCB 808 (359-1558 bp) and UCB 890 (369-1385 bp), with sizes close to those detected in this study, that ranged from 350-2000 bp. The percentage of polymorphism (90.3%) was close to that reported by Cavallari et al (2006), 89.8%, who studied populations of Ecolirium biflorum (Bromeliaceae) with RAPD markers.…”

“…According to Murawski & Hamrick (1990), the mean The ISSR markers used in this study therefore generated much higher values than those reported by Murawski & Hamrick (1990) for monocotyledons. The same primers used in this study generated similar results in Grevillea (Proteaceae) (Pharmawati et al, 2004) and Cycas guizhouensis (Cycadaceae) (Xiao et al 2004). In these studies the number of fragments ranged from 8-23 (UCB 808), 7-10 (UCB 842) and 8-24 (UCB 890).…”

Molecular analysis of some endemic and near-endemic medicinal plants located at Saint Katherine, Egypt

“…The technique is rapid, simple, inexpensive and more reproducible than RAPD [23]. ISSR used to study the genetic diversity of plants for examples; Nepeta [24], thyme [25] , Mentha aquatica L. [26], satureja [27], Salvia [28], Dracocephalum thymiflorum L. [29] and Thymus [30].…”

Phylogenic Study of Twelve Species of Phyllanthus Originated from India through Molecular Markers for Conservation

“…Both RAPD and IS-SR marker, based on PCR techniques have proven to be a reliable, easy to generate, inexpensive and versatile set of marker that rely on repeatable amplification of DNA se-quence using single primers. The RAPD and ISSR markers can be used in the study of the genetic variability of species or natural populations and in the identification of genotypes [7][8][9][10][11][12][13][14]. In this communication, we report the feasibility of PCR-based DNA (RAPD and ISSR) marker for phylogeny study and identification for conservation of Phyllanthus species.…”