Application of selective ion monitoring to the analysis of molecular species of vegetable oil triacylglycerols separated by open‐tubular column GLC on a methylphenylsilicone phase at high temperature

Abstract

Analyses of the molecular species of authentic triacylglycerols and of soybean triacylglycerols, which were separated on a fused silica open‐tubular column coated with a methylphenylsilicone gum stationary phase, were carried out by selective ion monitoring mass spectrometry. It was determined that peak assignments could be made by selecting certain characteristic ions with the same retention time on the SIM profile, that is, three ions of the type RCO+ corresponding to the fatty acyl residues (R1CO+, R2CO+ and R3CO+) and the corresponding three M‐acyl ions ([M‐OCOR1]+, [M‐OCOR2]+ and [M‐OCOR3]+ instead of the molecular ion. The yield of [RCO‐1]+ and [RCO‐2]+ and relating these to the unsaturated fatty acyl residues is inconvenient for an exact peak assignment. The SIM method was further applied to the peak assignment of the triacylglycerols from palm, cottonseed and safflower oils and certain new fatty acid combinations are proposed.

Quantification of Generic SALATRIM Material in Foods by the Combination of Nontraditional Crude Fat Extraction and Short Nonpolar Column High-Temperature Capillary Gas Chromatography

“…Since then, the application of GC-mass spectrometry in lipid characterization with either packed or capillary column has been reported by many researchers. For example, Oshima et al (1989) used a phenylmethylsilicone stationary phase column and GC-MS with selective ion monitoring to analyze the molecular species of vegetable oil triacylglycerols. More recently, Huang et al (1994a) used a 65% phenylmethylsilicone capillary column GC combined with ammonia chemical ionization mass spectrometry to identify various SALATRIM triacylglycerols.…”

Investigation of ultrahigh‐performance liquid chromatography/travelling‐wave ion mobility/time‐of‐flight mass spectrometry for fast profiling of fatty acids in the high Arctic sea surface microlayer

“…Thin‐layer chromatography (TLC) was one of the earliest methods published regarding detection and quantification of FAs . Gas chromatography (GC) with flame ionization detection (FID) and GC with mass spectrometry (MS) have become routine methods for determination of FAs, mostly employing derivatization into methyl esters after solvent extraction in order to enable sufficient separation on common GC stationary phases . Other methods have been based on high‐performance liquid chromatography (HPLC) utilizing either UV, fluorescence, refractive index, evaporative light scattering or MS for detection.…”

Quantification of Generic Salatrim in Foods Containing Salatrim and Other Fats Having Medium- and Long-Chain Fatty Acids

“…is the easy coupling of GC with mass spectrometry. Mass spectrometry with ammonia chemical ionization has been used in the identification of triacylglycerols in several laboratories (Murata, 1977;Murata and Takahashi, 1977;Oshima et al, 1989;Huang et al, 1994a). More recently, Huang et al (1995) used a short SIM-DIST capillary column GC combined with ammonia chemical ionization mass spectrometry to confirm various Salatrim components.…”