Application of sequential integration for metabolic engineering of 1,2-propanediol production in yeast

Lee, Wonkyu; DaSilva, Nancy A.

doi:10.1016/j.ymben.2005.09.001

Cited by 41 publications

(44 citation statements)

References 11 publications

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“…Indeed, strong enzyme overproduction may considerably exceed the level that is optimal for the metabolic engineering goal. There are several examples of target phenotype optimization achieved by moderate rather than strong multicopy gene expression in S. cerevisiae (147,150,196,345). Moreover, one example even demonstrates that very low enzyme activity can be optimal.…”

Section: Fine-tuning Gene Expressionmentioning

confidence: 99%

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Progress in Metabolic Engineering of Saccharomyces cerevisiae

Nevoigt

2008

Microbiol Mol Biol Rev

529

333

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SUMMARY The traditional use of the yeast Saccharomyces cerevisiae in alcoholic fermentation has, over time, resulted in substantial accumulated knowledge concerning genetics, physiology, and biochemistry as well as genetic engineering and fermentation technologies. S. cerevisiae has become a platform organism for developing metabolic engineering strategies, methods, and tools. The current review discusses the relevance of several engineering strategies, such as rational and inverse metabolic engineering, evolutionary engineering, and global transcription machinery engineering, in yeast strain improvement. It also summarizes existing tools for fine-tuning and regulating enzyme activities and thus metabolic pathways. Recent examples of yeast metabolic engineering for food, beverage, and industrial biotechnology (bioethanol and bulk and fine chemicals) follow. S. cerevisiae currently enjoys increasing popularity as a production organism in industrial (“white”) biotechnology due to its inherent tolerance of low pH values and high ethanol and inhibitor concentrations and its ability to grow anaerobically. Attention is paid to utilizing lignocellulosic biomass as a potential substrate.

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Section: Fine-tuning Gene Expressionmentioning

confidence: 99%

“…Recently, two E. coli genes, mgs and gldA, which encode methylglyoxal synthase and glycerol dehydrogenase, respectively (Fig. 3), were each integrated into the S. cerevisiae genome under the control of the CUP1 promoter (196). Different copy numbers of both genes were tested in combination.…”

Section: Production Of Fine and Bulk Chemicalsmentioning

confidence: 99%

Progress in Metabolic Engineering of Saccharomyces cerevisiae

Nevoigt

2008

Microbiol Mol Biol Rev

529

333

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“…Recent processes for the microbial production of 1,2-PDO from sugars, predominantly glucose, have used microorganisms such as Thermoanaerobacterium thermosaccharolyticum (Altaras et al, 2001;Sanchez-Riera et al, 1987), Clostridium sphenoides (Trandin and Gottschalk, 1985), Saccharomyces cerevisiae (Jung et al, 2008;Lee and DaSilva, 2006), and E. coli Cameron, 1999, 2000;Huang et al, 1999). Utilizing batch cultures, 1,2-PDO titers from glucose ranged from 0.49 g/L with E. coli (Altaras and Cameron, 1999) to 1.11 g/L with S. cerevisiae (Jung et al, 2008) and 9.0 g/L with T. thermosaccharolyticum (Sanchez-Riera et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Metabolic engineering of Escherichia coli for the production of 1,2‐propanediol from glycerol

Clomburg

González

2010

Biotech & Bioengineering

121

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Due to its availability, low-price, and high degree of reduction, glycerol has become an attractive carbon source for the production of fuels and reduced chemicals. Using the platform we have established from the identification of key pathways mediating fermentative metabolism of glycerol, this work reports the engineering of Escherichia coli for the conversion of glycerol into 1,2-propanediol (1,2-PDO). A functional 1,2-PDO pathway was engineered through a combination of overexpression of genes involved in its synthesis from the key intermediate dihydroxyacetone phosphate (DHAP) and the manipulation of the fermentative glycerol utilization pathway. The former included the overexpression of methylglyoxal synthase (mgsA), glycerol dehydrogenase (gldA), and aldehyde oxidoreductase (yqhD). Manipulation of the glycerol utilization pathway through the replacement of the native E. coli PEP-dependent dihydroxyacetone kinase (DHAK) with an ATP-dependent DHAK from C. freundii increased the availability of DHAP allowing for higher 1,2-PDO production. Analysis of the major fermentative pathways identified ethanol as a required co-product while increases in 1,2-PDO titer and yield were achieved through the disruption of the pathways for acetate and lactate production. Combination of these key metabolic manipulations resulted in an engineered E. coli strain capable of producing 5.6 g/L 1,2-PDO, at a yield of 21.3% (w/w). This strain also performed well when crude glycerol, a by-product of biodiesel production, was used as the substrate. The titer and yield achieved in this study were favorable to those obtained with the use of E. coli for the production of 1,2-PDO from common sugars.

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“…This copper-activated TA binds specifi cally to a regulatory UAS of P CUP1 and activates the expression of the metallothionein. The copper-induced gene expression systems have been used frequently for heterologous protein production [ 47 , 48 ] and to a lesser extent in metabolic engineering such as for the production of methyl benzoate [ 49 ] and 1,2-propanediol [ 50 ].…”

Section: Regulated Promotersmentioning

confidence: 99%

Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae

Hubmann

Thevelein

Nevoigt

2014

Methods in Molecular Biology

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The ease of highly sophisticated genetic manipulations in the yeast Saccharomyces cerevisiae has initiated numerous initiatives towards development of metabolically engineered strains for novel applications beyond its traditional use in brewing, baking, and wine making. In fact, baker's yeast has become a key cell factory for the production of various bulk and fine chemicals. Successful metabolic engineering requires fine-tuned adjustments of metabolic fluxes and coordination of multiple pathways within the cell. This has mostly been achieved by controlling gene expression at the transcriptional level, i.e., by using promoters with appropriate strengths and regulatory properties. Here we present an overview of natural and modified promoters, which have been used in metabolic pathway engineering of S. cerevisiae. Recent developments in creating promoters with tailor-made properties are also discussed.

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Application of sequential integration for metabolic engineering of 1,2-propanediol production in yeast

Cited by 41 publications

References 11 publications

Progress in Metabolic Engineering of Saccharomyces cerevisiae

Progress in Metabolic Engineering of Saccharomyces cerevisiae

Metabolic engineering of Escherichia coli for the production of 1,2‐propanediol from glycerol

Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae

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