Application of SFC for the characterization of formulated drug products

Ferguson, Paul; Cross, Rebecca; Schad, Gesa

doi:10.1016/b978-0-323-88487-7.00009-7

Cited by 2 publications

(1 citation statement)

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“…Although there are studies evaluating the composition and potential applications of polyphenolic compounds from the leaves of A. angustifolia , establishing optimal extraction conditions for these compounds without compromising their antioxidant properties has not been addressed. Supercritical fluid extraction (SFE) is a green extraction technology that separates analytes from a complex matrix (e.g., vegetal or food samples) based on their relative solubility [ 14 ]. Reportedly, SFE yields natural products with a high content of polyphenolic compounds using short extraction periods, making it a more efficient and less time-consuming process [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Agave angustifolia Haw. Leaves as a Potential Source of Bioactive Compounds: Extraction Optimization and Extract Characterization

Bermúdez-Bazán,

Estarrón-Espinosa,

Castillo-Herrera

et al. 2024

Molecules

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The leaves of Agave angustifolia Haw. are the main agro-waste generated by the mezcal industry and are becoming an important source of bioactive compounds, such as phenolic compounds, that could be used in the food and pharmaceutical industries. Therefore, the extraction and identification of these phytochemicals would revalorize these leaf by-products. Herein, maceration and supercritical carbon dioxide (scCO2) extractions were optimized to maximize the phenolic and flavonoid contents and the antioxidant capacity of vegetal extracts of A. angustifolia Haw. In the maceration process, the optimal extraction condition was a water–ethanol mixture (63:37% v/v), which yielded a total phenolic and flavonoid content of 27.92 ± 0.90 mg EAG/g DL and 12.85 ± 0.53 µg QE/g DL, respectively, and an antioxidant capacity of 32.67 ± 0.91 (ABTS assay), 17.30 ± 0.36 (DPPH assay), and 13.92 ± 0.78 (FRAP assay) µM TE/g DL. Using supercritical extraction, the optimal conditions for polyphenol recovery were 60 °C, 320 bar, and 10% v/v. It was also observed that lower proportions of cosolvent decreased the polyphenol extraction more than pressure and temperature. In both optimized extracts, a total of 29 glycosylated flavonoid derivatives were identified using LC-ESI-QTof/MS. In addition, another eight novel compounds were identified in the supercritical extracts, showing the efficiency of the cosolvent for recovering new flavonoid derivatives.

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Section: Introductionmentioning

confidence: 99%