Application of statistical experimental design for optimization of physiological factors and their influences on production of pullulan by Aureobasidium pullulans HP-2001 using an orthogonal array method

Gao, Wa; Chung, Chong-Pyoung; Li, Jianhong; Lee, Jin Woo

doi:10.1007/s11814-011-0107-4

Cited by 9 publications

(2 citation statements)

References 22 publications

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“…Hence, NaCl in the production medium could promote energy requirement for microbial growth and enhance pullulan yield. 45 A study on optimization of mineral salts using a statistical approach in the fermentation medium reported enhanced pullulan production by A. pullulans HP-2001. 43 Yu et al.…”

Section: Resultsmentioning

confidence: 99%

Valorization of cassava waste for pullulan production by Aureobasidium pullulans MTCC 1991

Viveka¹,

Varjani

Nakkeeran³

2020

Energy & Environment

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The interest in pullulan produced from industrial wastes has substantially increased due to its industrial applications. The major constraint in pullulan production is expensive and low yield. In this study, attempts were made on the evaluation of media components for maximum pullulan production from Aureobasidium pullulans MTCC 1991 using cassava waste in solid-state fermentation. A statistical approach was scrutinized to evaluate the effects of yeast extract, K2HPO4, (NH4)2SO4, MgSO4⋅7H2O, and NaCl on pullulan production. The optimal conditions eliciting the maximum pullulan yield (6.45 g pullulan/L of culture broth) were found to be 0.6 g/L yeast extract, 3 g/L K2HPO4, 0.2 g/L (NH4)2SO4, 0.3 g/L MgSO4⋅7H2O, and 1 g/L NaCl. The produced pullulan was extracted using the solvent precipitation method and characterized by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy spectroscopic analysis that confirmed pullulan with linear α (1–6) linkage. The results revealed that cassava waste could be used as an effective carbon source for the production of pullulan.

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Section: Resultsmentioning

confidence: 99%

Valorization of cassava waste for pullulan production by Aureobasidium pullulans MTCC 1991

Viveka¹,

Varjani

Nakkeeran³

2020

Energy & Environment

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“…Moreover, two methods indicated that the optimal concentrations of four salts for cell growth were different from those for production of CMCase. The optimal concentrations of salts in the medium for cell growth were reported to be different from those for the production of pullulan by A. pullulan [6]. Moreover, the optimal concentrations of salts for cell growth and production of pullulan varied with concentrations of carbon and nitrogen sources [5].…”

Section: Optimization Of Salts Using Response Surface Methodsmentioning

confidence: 99%

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Lee¹,

Kim²,

Gao³

et al. 2011

Journal of Life Science

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The optimal concentrations of salts in medium for cell growth and the production of carboxymethylcellulase (CMCase) by a recombinant E. coli JM109/DL-3 were established using two statistical methods: orthogonal array method (OAM) and response surface method (RSM). The analysis of variance (ANOVA) of data based on OAM indicated that K2HPO4 gave maximum sum of square (S) and percentage contribution (P) for cell growth as well as production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 in medium for cell growth extracted by Qualitek-4 (W32b) Software were 10.0, 1.0, 0.2, and 0.6 g/l, respectively, whereas those for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.0, 0.4, and 0.6 g/l. The analysis of variance (ANOVA) resulting from RSM indicated that a highly significant salt for cell growth was K2HPO4 ("probe>F" less than 0.0001), whereas K2HPO4 and MgSO4•7H2O were significant for the production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 for cell growth extracted by Design Expert Software were 7.44, 1.08, 0.22, and 0.88 g/l, respectively, whereas those for production of CMCase were 5.84, 0.69, 0.28, and 0.54 g/l. The optimal concentrations of salts and their influences on cell growth and production of CMCase extracted by OAM were almost the same as those by RSM. Production of CMCase by a recombinant E. coli JM109/DL-3 under optimized concentration of salts was 1.93 times higher than that by Bacillus amyloliquifaciens DL-3.

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Selection and Utilization of Agro-industrial Waste for Biosynthesis and Hyper-Production of Pullulan: A Review

Mishra¹,

Zamare²,

Manikanta³

2017

Biosynthetic Technology and Environmental Challenges

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Application of statistical experimental design for optimization of physiological factors and their influences on production of pullulan by Aureobasidium pullulans HP-2001 using an orthogonal array method

Cited by 9 publications

References 22 publications

Valorization of cassava waste for pullulan production by Aureobasidium pullulans MTCC 1991

Valorization of cassava waste for pullulan production by Aureobasidium pullulans MTCC 1991

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Selection and Utilization of Agro-industrial Waste for Biosynthesis and Hyper-Production of Pullulan: A Review

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