Application of Stem Cells in Oral Disease Therapy: Progresses and Perspectives

Yang, Bo; Qiu, Yi; Zhou, Niu; Ouyang, Hong; Ding, Junjun; Cheng, Bin; Sun, Jianbo

doi:10.3389/fphys.2017.00197

Cited by 48 publications

(42 citation statements)

References 151 publications

(136 reference statements)

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“…Dental stem cells serve as a reservoirs dedicated to replenishing damaged structures. The different dental stem cells have dedicated names based on their location and regenerative purpose ( Figure 5) [36]. However, many regenerative applications are being developed that use dental stem cells for purposes other than dental regeneration, since they have proven to have multi-differentiation potency [37][38][39][40][41].…”

Section: Stem Cells In the Oral Cavitymentioning

confidence: 99%

Attachment and spatial organisation of human mesenchymal stem cells on poly(ethylene glycol) hydrogels

Chahal

Schweikle

Heyward

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

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Strategies that enable hydrogel substrates to support cell attachment typically incorporate either entire extracellular matrix proteins or synthetic peptide fragments such as the RGD (arginine-glycine-aspartic acid) motif. Previous studies have carefully analysed how material characteristics can affect single cell morphologies. However, the influence of substrate stiffness and ligand presentation on the spatial organisation of human mesenchymal stem cells (hMSCs) have not yet been examined. In this study, we assessed how hMSCs organise themselves on soft (E = 7.4-11.2 kPa) and stiff (E = 27.3-36.8 kPa) poly(ethylene glycol) (PEG) hydrogels with varying concentrations of RGD (0.05-2.5 mM). Our results indicate that hMSCs seeded on soft hydrogels clustered with reduced cell attachment and spreading area, irrespective of RGD concentration and isoform. On stiff hydrogels, in contrast, cells spread with high spatial coverage for RGD concentrations of 0.5 mM or higher. In conclusion, we identified that an interplay of hydrogel stiffness and the availability of cell attachment motifs are important factors in regulating hMSC organisation on PEG hydrogels. Understanding how cells initially interact and colonise the surface of this material is a fundamental prerequisite for the design of controlled platforms for tissue engineering and mechanobiology studies.

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Section: Stem Cells In the Oral Cavitymentioning

confidence: 99%

Attachment and spatial organisation of human mesenchymal stem cells on poly(ethylene glycol) hydrogels

Chahal

Schweikle

Heyward

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

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“…Pulp vitality was assessed by studies on a continuous and an event-based scale, which is why two different meta-analyses were performed. Although the studies non-uniformly used autogenous or allogenic cellular sources for their experimental designs, it seems reasonable to assume that this factor would have only a negligible impact on the compared outcomes (Yang et al 2017). Still, three studies employed growth/differentiation factors to augment the regenerative potential of the transplanted stem/ progenitor cells: a cocktail of vascular endothelial growth factor (VEGF-2), basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF), nerve growth factor (NGF), bone morphogenetic protein-7 (BMP-7) (El Ashiry et al 2018) and granulocyte colony-stimulating factor (G-CSF) (Iohara et al 2013(Iohara et al , 2014.…”

Section: Figurementioning

confidence: 99%

Stem/progenitor cell‐mediated pulpal tissue regeneration: a systematic review and meta‐analysis

et al. 2019

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Background Stem/progenitor cell‐mediated pulpal regeneration could represent a promising therapeutic alternative in the field of clinical endodontics. Aim The present study aimed to systematically assess and meta‐analyse dental pulpal tissue regeneration, pulpal vitality and apical healing after the transplantation of stem/progenitor cells versus no transplantation. Data sources MEDLINE, Cochrane CENTRAL and EMBASE were searched up to January 2019 for animal experiments and human trials evaluating the pulpal transplantation of stem/progenitor cells. Cross‐referencing and hand search were additionally performed. Study eligibility criteria, participants and interventions Based on randomized controlled clinical trials (RCTs) or controlled clinical trials (CCTs), conducted in animals or humans, the effect of the transplantation of stem/progenitor cells compared to no transplantation on pulpal tissue regeneration, pulpal vitality and apical healing was examined. Study appraisal and synthesis methods The primary outcome was histologically determined pulpal tissue regeneration, whilst pulpal vitality and apical healing were secondary outcomes. The SYstematic Review Centre for Laboratory animal Experimentation (SYRCLE) guidelines and the revised Cochrane risk of bias tool (RoB 2.0) were used for risk‐of‐bias assessment. Pooled standardized differences in means (SDM) and 95% confidence intervals (95% CI) were calculated using random‐effects meta‐analyses. Results From 2834 identified articles, eight animal experiments (82 animals with 336 experimental pulpal defects) and one human trial (40 humans with 40 pulpal defects) were included. Risk of bias of most animal studies was high, whilst the human trial revealed ‘some concerns’. Stem/progenitor cell‐transplanted pulps demonstrated significantly increased pulpal tissue regeneration compared with controls (SDM [95%CI]: 6.29 [3.78–8.80]). Limitations Data on pulpal vitality and apical healing were sparse and inconsistent. Heterogeneity across studies was substantial, publication bias was present, and mainly indirect, surrogate outcome measures were applied. The overall strength of evidence was very low. Conclusions and implications of key findings The transplanation of stem/progenitor cells shows promise for pulp regeneration, whilst clinical routine application is still not in reach. Further investigations, employing a comprehensive set of outcomes including those demonstrating functional pulp regeneration relevant for patient‐centred care, are required.

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“…4). ко, поскольку основным субстратом для продолженного роста корня служат стволовые клетки апикального сосочка [3], можно предположить, что важную роль в успехе регенерации пульпы играет его сохранение, а значитдлительность и интенсивность воспалительного процесса в периапикальных тканях. У детей с длительно текущим воспалительным процессом в периодонте (что отражает срок, прошедший от момента травмы до констатации некроза пульпы, а также размер ОПП) реваскуляризация показала полную эффективность в отношении разрешения ОПП (100% случаев), но эффективность формирования твердотканного барьера и продолженного роста корня прямо коррелировала с наличием и размером ОПП, а также с наличием признаков наружной резорбции корня.…”

Section: стоматология 4 2018unclassified

Pulp revascularization for the management of avulsed incisors with immature roots and pulp necrosis

Korolenkova

Рахманова

2018

Stomat.

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The aim of the study was to assess the efficacy of pulp revascularization (PR) in children with pulp necrosis in immature roots incisors with the history of avulsion. The study group comprised 11 children (4 girls and 7 boys aged 86-133 months) with 13 traumatized incisors with pulp necrosis. Mean age at the time of injury was 98.0 months. In 5 children pulp necrosis was associated with extensive periapical lesion (PL, 5-26 mm diameter), 4 children with the history of inadequate or ineffective endodontic treatment had radiological signs of external root resorption. PR was performed according to standard protocol and the results were assessed 3, 6, 12, 18 and 24 months after the procedure. All 13 teeth were clinically asymptomatic 24 months after PR, but continuous root growth and/or apexification was seen in 9 teeth only. PL resolved in all cases, root apexification correlated positively with the absence of PL and external root resorption, continuous root growth and dentinal walls thickening were less evident in patients with pulp necrosis at earlier stages of root development. No progression of external root resorption was observed. PR is a useful tool for the treatment of immature root incisors with pulp necrosis after teeth avulsion but extensive PL and external root resorption prevent adequate apexification probably because of the apical papilla necrosis.

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Application of Stem Cells in Oral Disease Therapy: Progresses and Perspectives

Cited by 48 publications

References 151 publications

Attachment and spatial organisation of human mesenchymal stem cells on poly(ethylene glycol) hydrogels

Attachment and spatial organisation of human mesenchymal stem cells on poly(ethylene glycol) hydrogels

Stem/progenitor cell‐mediated pulpal tissue regeneration: a systematic review and meta‐analysis

Pulp revascularization for the management of avulsed incisors with immature roots and pulp necrosis

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