Application of Stereomicroscope in the Pre-Analytic Macroscopic Examination of Biopsy Specimens

Mathew, Jacob; Mohan, Neena; Fenn, Saramma Mathew; Rajathi, P.; Suryagopan, P; Vishalini, L

doi:10.7860/jcdr/2019/42116.13182

Cited by 2 publications

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“…Even the dimensional changes that distort the specimen caused as result of tissue processing can be appreciated better under the stereomicroscope. [3] The present case in this article is a patient diagnosed with oral mucocele. Mucoceles are the most common benign minor salivary gland disorders and the second most common benign soft-tissue masses of the oral cavity, following fibrous hyperplasia.…”

Section: Discussionmentioning

confidence: 96%

Harnessing stereomicroscopy in pathological diagnosis: A case report on oral mucocele

Nasrin

Jacob

Rajathi

et al. 2022

JADE

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The pre-analytic phase of a histopathology laboratory begins with the gross examination of biopsy specimens for orientation and dissecting the area of suspicion in histological diagnosis. This step is routinely performed by junior residents or laboratory technicians and very rarely by the diagnosing pathologists. Following conventional method of grossing, the macroscopic features recorded are subjective and limited and the photographs taken of the surgical specimens are often pixilated when magnified. The present case report describes the application of stereomicroscopy in gross examination of a case of oral mucocele and its function in histologic diagnosis.

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Section: Discussionmentioning

confidence: 96%

Harnessing stereomicroscopy in pathological diagnosis: A case report on oral mucocele

Nasrin

Jacob

Rajathi

et al. 2022

JADE

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Metalens-based stereoscopic microscope

et al. 2022

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Stereoscopic microscopy is a promising technology to obtain three-dimensional microscopic images. Such microscopes are based on the parallax effect, and as such require two lenses to focus at two different points. Geometrical constraints, however, restrict their numerical apertures to about 0.2, thus limiting the system’s resolution. Higher numerical apertures ( ∼ 0.35 ) can be achieved with designs using only one bulk lens, but such systems are ∼ 10 times more costly than the conventional ones. Thus, there is a pressing need for alternative solutions to improve the resolution of stereoscopic systems. Here, we show that high-resolution and low-cost stereoscopic systems can be obtained using birefringent single-layer metalenses. We design and fabricate a birefringent metalens operating at 532 nm with a numerical aperture as high as 0.4. The metalens is then used to demonstrate high-resolution stereoscopic imaging of biological samples. The microscopic images are further displayed and perceived vividly in an autostereoscopic display. Our demonstration paves the way to a new strategy to achieve high-resolution and low-cost stereoscopic microscopes.

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Application of Stereomicroscope in the Pre-Analytic Macroscopic Examination of Biopsy Specimens

Cited by 2 publications

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Harnessing stereomicroscopy in pathological diagnosis: A case report on oral mucocele

Harnessing stereomicroscopy in pathological diagnosis: A case report on oral mucocele

Metalens-based stereoscopic microscope

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