Application of Taguchi’s method in the optimization of bridging efficiency between confluent and fresh microcarriers in bead-to-bead transfer of Vero cells

Luo, Fengshan; Sun, Hai‐Ying; Qi, Nianmin

doi:10.1007/s10529-007-9604-2

Cited by 16 publications

(9 citation statements)

References 15 publications

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“…As initial microcarrier colonization has been shown to improve the efficiency of expansion on microcarriers, 51, 60 we examined the first 8 h after inoculation to observe cell attachment. Upon sterilization, microcarriers were rinsed with PBS and equilibrated in prewarmed serum‐free medium.…”

Section: Resultsmentioning

confidence: 99%

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Efficient suspension bioreactor expansion of murine embryonic stem cells on microcarriers in serum‐free medium

Alfred

Radford

Fan

et al. 2011

Biotechnology Progress

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Large numbers of cells will be required for successful embryonic stem cell (ESC)-based cellular therapies or drug discovery, thus raising the need to develop scaled-up bioprocesses for production of ESCs and their derived progeny. Traditionally, ESCs have been propagated in adherent cultures in static flasks on fibroblasts layers in serum-containing medium. Direct translation of two-dimensional flatbed cultures to large-scale production of the quantities of cells required for therapy simply by increasing the number of dishes or flasks is not practical or economical. Here, we describe successful scaled-up production of ESCs on microcarriers in a stirred culture system in a serum-free medium. Cells expanded on CultiSpher S, Cytodex 3, and Collagen microcarriers showed superior cell-fold expansions of 439, 193, and 68, respectively, without excessive agglomeration, compared with 27 in static culture. In addition, the ESCs maintained their pluripotency after long-term culture (28 days) in serum-free medium. This is the first time mESCs have been cultured on microcarriers without prior exposure to serum and/or fibroblasts, while also eliminating the excessive agglomeration plaguing earlier studies. These protocols provide an economical, practical, serum-free means for expanding ESCs in a stirred suspension bioprocess.

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Section: Resultsmentioning

confidence: 99%

“…Bridging (but not excessive agglomeration) has been used previously as a method of repopulating empty beads when bioprocessing other cell types on microcarriers. 60, 62, 63 Excessive agglomeration leads to the formation of huge clumps leading to diffusion gradients, which are undesirable in cell cultures.…”

Section: Resultsmentioning

confidence: 99%

Efficient suspension bioreactor expansion of murine embryonic stem cells on microcarriers in serum‐free medium

Alfred

Radford

Fan

et al. 2011

Biotechnology Progress

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“…Intermittent agitation compared to continuous agitation has shown positive effect on bead-to-bead transfer . Different intermittent agitation conditions have also been studied and showed that resting time and stirring time were both important in addition to the interaction between stirring time and resting time, and stirrer speed and resting time (Luo et al 2008). Experienec with myoblasts showed more efficient culture with continuous agitation, however with a lag-phase during which no increase in cell density occurred.…”

Section: Optimization Of Culture Conditionsmentioning

confidence: 99%

Alternatives for large-scale production of cultured beef: A review

Moritz

Verbruggen

Post

2015

Journal of Integrative Agriculture

107

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Cultured beef is a method where stem cells from skeletal muscle of cows are cultured in vitro to gain edible muscle tissue. For large-scale production of cultured beef the culture technique needs to become more efficient than today's 2D standard technique that was used to make the first cultured hamburger. Options for efficient large-scale production of stem cells are to culture cells on microcarriers, either in suspension or in a packed bed bioreactor, or to culture aggregated cells in suspension. We discuss the pros and cons of these systems as well as the possibilities to use the systems for tissue culture. Either of the production systems needs to be optimized to achieve an efficient production of cultured beef. It is anticipated that the optimization of large-scale cell culture as performed for other stem cells can be translated into successful protocols for bovine satellite cells resulting in resource and cost efficient cultured beef

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“…Hence, the total number of running the problem is 2,430,000 trials. In this situation, in order to decrease the number of experiments, the Taguchi experimental design as one of the successfully applied tools is selected for parameter tuning [4,15,26,40]. Taguchi experimental technique was first introduced by Taguchi in 1960s.…”

Section: Performance Assessmentmentioning

confidence: 99%

Scheduling of printed circuit board (PCB) assembly systems with heterogeneous processors using simulation-based intelligent optimization methods

Noroozi

Mokhtari

2014

Neural Comput & Applic

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The complexity of printed circuit boards (PCBs), as an important sector of the electronics manufacturing industry, has increased over the last three decades. This paper focuses on a practical application observed at a PCB assembly line of electronics manufacturing facility. It is shown that this problem is equivalent to a flowshop scheduling with multiple heterogeneous batch processors where processors can perform multiple tasks as long as the sizes of jobs in a batch do not violate the processors' capacity. The equivalent problem is mathematically formulated as a mixed integer programming model. Then, a Monte Carlo simulation is incorporated into high-level genetic algorithm-based intelligent optimization techniques to assess the performance of makespan-oriented system under uncertain processing times. At each iteration of algorithm, the output of simulator is used by optimizers to provide online feedbacks on the progress of the search and direct the search toward a promising solution zone. Furthermore, various parameters and operators of the algorithm are discussed and calibrated by means of Taguchi statistical technique. The result of extensive computational experiments shows that the solution approach gives high-quality solutions in reasonable computational time.

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Application of Taguchi’s method in the optimization of bridging efficiency between confluent and fresh microcarriers in bead-to-bead transfer of Vero cells

Cited by 16 publications

References 15 publications

Efficient suspension bioreactor expansion of murine embryonic stem cells on microcarriers in serum‐free medium

Efficient suspension bioreactor expansion of murine embryonic stem cells on microcarriers in serum‐free medium

Alternatives for large-scale production of cultured beef: A review

Scheduling of printed circuit board (PCB) assembly systems with heterogeneous processors using simulation-based intelligent optimization methods

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