Application of the direct epifluorescent filter technique as a rapid method in microbiological quality assurance in the meat industry

Qvist, Sven; Jakobsen, Marianne Uhre

doi:10.1016/0168-1605(85)90032-7

Cited by 25 publications

(3 citation statements)

References 3 publications

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“…The arrival of a new committed supplier resulted in the development of more purpose-built equipment such that the DEFT is easier to carry out. This has renewed interest and confidence in the technique such that its wider application is being investigated worldwide (Boisen, 1983;O'Connor et al, 1983;Qvist & Jakobsen, 1985;Suhren and Heeschen, 1985;Pettipher, 1987).…”

Section: Discussionmentioning

confidence: 99%

Observations on the introduction of the DEFT for the routine assessment of bacteriological quality

Easter

Kroll²,

Farr³

et al. 1987

Int J of Dairy Tech

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In the light of experiences gained and observations made in the dairy industry, the critical control points and factors that affect the operation of the DEFT (Direct Epifluorescent Filter Technique) in a routine laboratory were determined. Recommendations are made for the optimization of both the test protocol and the instrumentation, and the need for adequate operator training is strongly emphasized.

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Section: Discussionmentioning

confidence: 99%

Observations on the introduction of the DEFT for the routine assessment of bacteriological quality

Easter

Kroll²,

Farr³

et al. 1987

Int J of Dairy Tech

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“…The direct epifluorescent filter technique has been used for enumeration of microorganisms, mainly in food analyses (Pettipher and Rodrigues 1982;Qvist and Jakobsen 1985;Pettipher 1989). Acridine orange (AO), the most commonly used dye, stains the nucleic acids in different ways, but there is no consensus concerning the use of AO for distinction between viable and non-viable cells (Pettipher 1989).…”

Section: Offprint Requests To: M T O Barretomentioning

confidence: 99%

A kinetic method for calculating the viability of lactic starter cultures

Barreto

Melo

Almeida

et al. 1991

Appl Microbiol Biotechnol

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A simple method of assessing the viability of a lactic starter culture is presented. The method is based on the kinetics of growth of a culture during the lag and early exponential phases. The microorganisms used throughout this work were Lactobacillus spp. The method was tested with inocula samples of different ages and with samples taken during chemostat runs, at different dilution rates. The results obtained are similar to ones described in the literature for similar situations. The method is very easy to operate once the intrinsic parameters have been established (Xmax and /~max). It needs only standard laboratory equipment and is very economical.

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“…This has aroused considerable interest in developing rapid methods in the meat industry. The main reasons for this are the advantages related to the speedy release of products, and that rapid results in monitoring critical control points offer the possibility for early corrections during production (Qvist and Jakobsen, 1985). Finally, rapid results enable the manufacturers to select raw materials to conform to their microbiological quality standards and also there is a possibility for a quick assessment of meat products following transportation.…”

Section: Introductionmentioning

confidence: 99%

Survival and growth characteristics of bacteria in irradiated meat

Venugopal¹

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6.2 Introduction 15 8 6.3 Materials and Methods 160 6.3.1 Meat samples 6.3.2 Microbial recovery studies 6.3.3 Microbiological analyses 6.3.4 FCM analyses of spoiling pork patties 6.3.5 FCM analysis of retail samples 6.3.6 Quantification of bacterial cells using FCM 6.3.7 Evaluation of column materials 6.3.8 Clarification of meat suspensions for column material evaluation 6.3.8 Clarification of meat suspensions for flow cytometry 6.3.9 Flow cytometry 165 6.3.10 Staining protocol 165 6.3.12 Statistics 165 6.4 Results 165 6.4.1 Evaluation of column materials 165 6.4.2 Quantification of bacterial cells in meat homogenate using FCM 167

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Application of the direct epifluorescent filter technique as a rapid method in microbiological quality assurance in the meat industry

Cited by 25 publications

References 3 publications

Observations on the introduction of the DEFT for the routine assessment of bacteriological quality

Observations on the introduction of the DEFT for the routine assessment of bacteriological quality

A kinetic method for calculating the viability of lactic starter cultures

Survival and growth characteristics of bacteria in irradiated meat

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