“…C 0 is determined by the negative intercept of the curve with the abscissa axis (Muel & Lacroix, 1960;Rima, Lamotte & Joussot-Dubien, 1982).…”
Section: Spectrophotometric Methods For Quantification Of Atrazine Usimentioning
confidence: 99%
“…Dechlorinated atrazine was added as an internal standard for the calibration of the measurement (Muel & Lacroix, 1960;Rima, Lamotte, & Joussot-Dubien, 1982). Determination of the pH was done using a Mettler Toledo (OH, USA) pH-meter.…”
Section: Internal Standard Curve Addition and Recoverymentioning
The objective of this study is to develop a simple and economic spectrophotometric method for the quantitative determination of atrazine. This method is based on the complexation of atrazine derivatization (dechlorinated atrazine [DA]) with a mixture of formaldehyde and ketone compound, as described by Mannich reaction. The complex was determined by UV-Vis absorption measurement and the ketone compound used was the uranine due to its high coefficient absorption. The UV spectrum of the complex shows maxima of absorption at 207 nm and at 227 nm. An internal standard was used to quantify the atrazine. There is a good linearity between the absorbance and the concentration in the range of 0.1 -10 μg.mL -1 of atrazine. The recovery value was 97 % and the limit of detection was 0.01 µg.mL -1 . Real samples collected from irrigation local area were analyzed using this method and the estimated concentration of atrazine found in the mentioned river is 0.29 ± 0.011 μg.mL -1 .
“…C 0 is determined by the negative intercept of the curve with the abscissa axis (Muel & Lacroix, 1960;Rima, Lamotte & Joussot-Dubien, 1982).…”
Section: Spectrophotometric Methods For Quantification Of Atrazine Usimentioning
confidence: 99%
“…Dechlorinated atrazine was added as an internal standard for the calibration of the measurement (Muel & Lacroix, 1960;Rima, Lamotte, & Joussot-Dubien, 1982). Determination of the pH was done using a Mettler Toledo (OH, USA) pH-meter.…”
Section: Internal Standard Curve Addition and Recoverymentioning
The objective of this study is to develop a simple and economic spectrophotometric method for the quantitative determination of atrazine. This method is based on the complexation of atrazine derivatization (dechlorinated atrazine [DA]) with a mixture of formaldehyde and ketone compound, as described by Mannich reaction. The complex was determined by UV-Vis absorption measurement and the ketone compound used was the uranine due to its high coefficient absorption. The UV spectrum of the complex shows maxima of absorption at 207 nm and at 227 nm. An internal standard was used to quantify the atrazine. There is a good linearity between the absorbance and the concentration in the range of 0.1 -10 μg.mL -1 of atrazine. The recovery value was 97 % and the limit of detection was 0.01 µg.mL -1 . Real samples collected from irrigation local area were analyzed using this method and the estimated concentration of atrazine found in the mentioned river is 0.29 ± 0.011 μg.mL -1 .
“…C add is the known added concentration. The concentration C 0 is determined by the negative intercept of the extrapolated line with the x-axis (Muel and Lacroix, 1960;Rima et al, 1982). It is noted that this method requires a calibration curve for each sample.…”
Section: Spectrofluorometric Methods For Quantification Of Allrac-a-tomentioning
confidence: 99%
“…A standard addition method (Muel and Lacroix, 1960;Rima et al, 1982) was used to calibrate all-rac-a-tocopheryl acetate contents in olive oils. The standard was all-rac-a-tocopheryl acetate added in olive oil.…”
“…Moreover, the fluorescence spectrum of a-tocopherol extract of Zalooh, extracted following a chromatographic column, is examined on a spectrofluorophotometer at 292 nm excitation and 328 nm emission wavelength, and the amount of a-tocopherol present is dosed according to the internal addition method (Rima et al, 1982). We finally note that for the extraction of tocopherols, and before the HPLC or any other analysis, saponification is needed for samples high in fat in order to remove the fatty acids in excess (which is the case for Zalooh).…”
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