Application of the SPE reversed phase HPLC/MS technique to determine vitamin B12 bio-active forms in beef

Szterk, Arkadiusz; Roszko, Marek; Małek, Krystian; Czerwonka, Małgorzata; Waszkiewicz-Robak, B.

doi:10.1016/j.meatsci.2012.02.023

Cited by 46 publications

(37 citation statements)

References 27 publications

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“…Ionization of CN-Cbl using an ESI source leads to the formation of the ions 678.3 [M?2H] 2? , which was consist with that reported by Szterk [25]. In this study, only CNCbl could be detected as different kinds of vitamin B 12 were all translated to CN-Cbl.…”

Section: Resultssupporting

confidence: 91%

A newly isolated and identified vitamin B12 producing strain: Sinorhizobium meliloti 320

Dong

Fang

et al. 2016

Bioprocess Biosyst Eng

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Vitamin B12 (Cobalamin, VB12) has several physiological functions and is widely used in pharmaceutical and food industries. A new unicellular species was extracted from China farmland, and the strain could produce VB12 which was identified by HPLC and HPLC-MS/MS. 16S rDNA analysis reveals this strain belongs to the species Sinorhizobium meliloti and we named it S. meliloti 320. Its whole genome information indicates that this strain has a complete VB12 synthetic pathway, which paves the way for further metabolic engineering studies. The optimal carbon and nitrogen sources are sucrose and corn steep liquor (CSL) plus peptone. The optimal combination of sucrose and CSL was obtained by response surface methodology as they are the most suitable carbon and nitrogen sources, respectively. This strain could produce 140 ± 4.2 mg L(-1) vitamin B12 after incubating for 7 days in the optimal medium.

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Section: Resultssupporting

confidence: 91%

A newly isolated and identified vitamin B12 producing strain: Sinorhizobium meliloti 320

Dong

Fang

et al. 2016

Bioprocess Biosyst Eng

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“…Subsequently, the 6 batches of uncontained samples were used as a prediction set to test the method validation. The results showed that the overall prediction accuracy reached 94.67 ± 7.71%, and the AUC values were 0.98 ± 0.01-1.00 (Konieczk, & Namiesnik, 2010;Szterk, Roszko, & Cybulski, 2012;Szterk, Roszko, Małek, Czerwonka, & Waszkiewicz-Robak, 2012;. The AUC value is an indicator of the efficacy of the assessment system.…”

Section: Multivariate Statistical Analysismentioning

confidence: 99%

Global metabolite profiling and diagnostic ion filtering strategy by LC–QTOF MS for rapid identification of raw and processed pieces of Rheum palmatum L.

Liu

Xiao

et al. 2016

Food Chemistry

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“…Liquid chromatography (LC) is potentially useful for Cbl speciation, but the separation is complicated by the structural similarity, and the most commonly used UV detection is often inadequate due to the low natural levels occurring in biological samples and non‐supplemented foodstuffs . LC with inductively coupled plasma mass spectrometry provides good sensitivity for the 59 Co isotope but needs the complete chromatographic separation of the Cbl homologues; LC electrospray ionization‐tandem mass spectrometry shows better selectivity and sensitivity …”

Section: Introductionmentioning

confidence: 99%

4‐Chloro‐α‐cyanocinnamic acid is an efficient soft matrix for cyanocobalamin detection in foodstuffs by matrix‐assisted laser desorption/ionization mass spectrometry (MALDI MS)

et al. 2016

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4-Chloro-α-cyanocinnamic acid (ClCCA) is a very useful matrix able to give the protonated adduct [M+H](+) of intact cyanocobalamin (CNCbl) as the base peak (m/z 1355.58) in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). The only fragment observed is [M-CN + H](+•) formed through the facile (•) CN neutral loss reflecting the fairly low Co-C bond energy. All other investigated proton transfer matrices, including α-cyano-4-hydroxycinnamic acid, para-nitroaniline and 2,5-dihydroxybenzoic acid, give rise to a complete decyanation of CNCbl with concomitant formation of [M-CN + H](+•) , [M-CN + Na](+•) and [M-CN + K](+•) adducts at m/z 1329.57, 1351.55 and 1367.51, respectively. Depending on the matrix used, a variable degree of fragmentation involving the α-side axial ligand was observed. A plausible explanation of the specific behaviour of 4-chloro-α-cyanocinnamic acid as a soft matrix is discussed. Tandem mass spectra of both [M + H](+) and [M-CN + H](+•) ions were obtained and product ions successfully assigned. The possibility of detecting the protonated adduct of intact CNCbl was exploited in foodstuff samples such as cow milk and hen egg yolk by MALDI tandem MS upon sample extraction. We believe that our data provide strong basis for the application of MALDI tandem MS in the qualitative analysis of natural CNCbl, including fish, liver and meat samples. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Application of the SPE reversed phase HPLC/MS technique to determine vitamin B12 bio-active forms in beef

Cited by 46 publications

References 27 publications

A newly isolated and identified vitamin B12 producing strain: Sinorhizobium meliloti 320

A newly isolated and identified vitamin B12 producing strain: Sinorhizobium meliloti 320

Global metabolite profiling and diagnostic ion filtering strategy by LC–QTOF MS for rapid identification of raw and processed pieces of Rheum palmatum L.

4‐Chloro‐α‐cyanocinnamic acid is an efficient soft matrix for cyanocobalamin detection in foodstuffs by matrix‐assisted laser desorption/ionization mass spectrometry (MALDI MS)

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