Application of thiophilic membranes for the purification of monoclonal antibodies from cell culture media

Finger, U.B.; Thömmes, Jörg; Kinzelt, Dagmar; Kula, Maria‐Regina

doi:10.1016/0378-4347(94)00362-9

Cited by 25 publications

(8 citation statements)

References 25 publications

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“…Finger et al, [51], coupled thiophilic ligands onto regenerated cellulose membranes for the purification of monoclonal antibodies. The membranes were tested in two different modes: dead-end and cross-flow.…”

Section: Affinity Membranesmentioning

confidence: 99%

Membrane adsorbers as purification tools for monoclonal antibody purification

Boi

2007

Journal of Chromatography B

147

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Section: Affinity Membranesmentioning

confidence: 99%

Membrane adsorbers as purification tools for monoclonal antibody purification

Boi

2007

Journal of Chromatography B

147

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“…Q and other charged MA devices have been in development for chromatography purposes for more than 15 years (20–23). Different mechanisms to purify proteins used by resins and membranes have been compared and evaluated in several review articles (24–27). MA has been reported as a potentially advantageous tool to purify protein at a laboratory and pilot scale (28–33).…”

Section: Introductionmentioning

confidence: 99%

Basic Concepts in Q Membrane Chromatography for Large-Scale Antibody Production

2006

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The large-scale production of recombinant human monoclonal antibodies demands economical purification processes with high throughputs. In this article we briefly describe a common antibody process and evaluate the Q membrane adsorber for process-scale antibody production as an alternative to a Q-packed-bed column in a flow-through mode. The scientific concepts underlining Q membrane technology and its application are reviewed. The disadvantages and advantages of using Q membrane chromatography as a purification unit in large-scale production are discussed, including problems initially seen with the Q membrane scale-down model but solved with the invention of a new scale-down model. The new Q-membrane unit operation has a process capacity greater than 3,000 g/m(2) or 10.7 kg/L with a LRV over 5 for four model viruses. In this Review, a cost analysis illustrates that Q membrane chromatography is a viable alternative to Q column chromatography as a polishing step in a flow-through mode for process-scale antibody production.

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“…544 Synthesis of surfacefunctionalized rigid material for high-performance TAC has also been described; 147,545,546 TAC has also been carried out in the membrane format. 547 The TAC method is a typical on -off affinity technique. The sample is loaded onto the column in a 10 -100 mM buffer at neutral pH, to which a 0.5 -1 M solution of a cosmotropic salt, typically sodium, potassium, or ammonium sulfate, has been added.…”

Section: Salt-promoted/thiophilic Adsorption Chromatographymentioning

confidence: 99%

High‐Performance Liquid Chromatography of Biological Macromolecules

Irgum¹

2000

Encyclopedia of Analytical Chemistry

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Application of thiophilic membranes for the purification of monoclonal antibodies from cell culture media

Cited by 25 publications

References 25 publications

Membrane adsorbers as purification tools for monoclonal antibody purification

Membrane adsorbers as purification tools for monoclonal antibody purification

Basic Concepts in Q Membrane Chromatography for Large-Scale Antibody Production

High‐Performance Liquid Chromatography of Biological Macromolecules

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