Application of Ultra-High-Performance Liquid Chromatography/ Tandem Mass Spectrometry for the Measurement of Vitamin D in Infant Formula and Adult/Pediatric Nutritional Formula: First Action 2011.11

Abstract

In an effort to measure vitamin D, ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) was applied to samples. The use of UHPLC-MS/MS decreased the run time by 50%. The UHPLC-MS/MS achieved equal or better separation efficiency with complex food matrixes compared to HPLC-MS/MS. It was also observed that under the optimized conditions of UHPLC, all previtamins of vitamin D3, D2, and isotope-labeled vitamin D3 were baseline-separated from their corresponding vitamins. The sterol isomers found in complex food matrixes that interfere in the analysis were well separated from the analytes. The accuracy of the method was evaluated by analyzing National Institute of Standards and Technology Standard Reference Material 1849 infant reference material. The average vitamin D3 concentration was 0.251 +/- 0.012 microg/g. This showed excellent agreement with the certified value of 0.251 +/- 0.027 microg/g. The spike recovery study of a commercial infant formula matrix showed a range of recovery from 100 to 108%. The LOQ values determined were 0.0022 and 0.0028 microg/g for vitamins D3 and D2, respectively; LOD values were 0.00065 and 0.00083 microg/g for vitamins D3 and D2, respectively.

Vitamin D2 Supplementation Amplifies Eccentric Exercise-Induced Muscle Damage in NASCAR Pit Crew Athletes

“…The mushroom vitamin D 2 analysis has been described fully [24,25]. Briefly, mushroom powder samples underwent 3 h saponification at room temperature.…”

Development and optimization of an LC‐MS/MS‐based method for simultaneous quantification of vitamin D2, vitamin D3, 25‐hydroxyvitamin D2 and 25‐hydroxyvitamin D3

“…Although HPLC coupled with UV/VIS detector is typically used to measure vitamin D in food 8, 9 it lacks the desired level of specificity due to spectral interferences for complex biological matrices. On the other hand, HPLC coupled with MS (LC-MS) offers both increased sensitivity and selectivity 10 and minimizes interferences commonly seen from complex food matrices 11. Several isotope dilution LC-MS/MS methods have been developed for measuring 25(OH)D 2 and 25(OH)D 3 in plasma that reported LOQs range of 1.7–6.5 ng/mL 12–18.…”

Vitamin D: an essential component for skeletal health

“…These measure both 25(OH)D 2 and 25(OH)D 3 42–47 . However, the specificity and the sensitivity are markedly improved by using physical methods, such as high performance liquid chromatography and liquid chromatography–tandem mass spectrometry (LC/MS/MS) technology 48–52 …”