Application of ultra‐performance liquid chromatography coupled with mass spectrometry to metabonomic study on spontaneously hypertensive rats and intervention effects of Ping Gan prescription

Abstract

This study describes the metabonomic characters of the spontaneously hypertensive rats (SHR) and intervention effects of Ping Gan prescription. Ultra performance LC coupled with Q-TOF MS (UPLC/MS-Q-TOF) was employed to develop a metabonomic method of the plasma of SHR. There was a significant difference in metabolic profiling observed between predose group of Wistar Kyoto rats and model group (SHR) by using the principal components analysis (PCA). Some significant changes in metabolites such as LysoPC(22:6), LysoPC(20:4), LysoPC(18:1), cholylglycine, PE(P-16:0e/0:0), sphingosine-1-phosphate, and 2-oxo-4-methylthio butanoic acid were identified. These biochemical changes were associated with the disturbance in sphingolipid metabolism and fat metabolism, which would be helpful to further understand the essence of hypertension and the therapeutic mechanism of Ping Gan prescription. This study suggests that the metabonomic method may be a valuable and feasible tool to explore the therapeutic effect and mechanism of traditional Chinese medicine (TCM).

Prehypertension-Associated Elevation in Circulating Lysophosphatidlycholines, Lp-PLA2 Activity, and Oxidative Stress

“…Several metabolomic studies have been published that investigate the metabolic effects of hypertension [5]–[7]. These studies reported abnormalities in gender-linked steroid patterns [5] or lipid metabolism [6], [7]. Hypertension and its underlying pathophysiology may be present for years before clinical diagnosis, at which time irreversible pathology has already occurred.…”

Quadrupole time-of-flight mass spectrometry as a powerful tool for demystifying traditional Chinese medicine

“…Biochemical changes supporting the intervention effect of Ping Gan prescription for spontaneous hypertension treatment were reported to be associated with disturbance in the sphingolipid and fat metabolisms [83].…”

UHPLC-MS for the analytical characterization of traditional Chinese medicines

“…UHPLC-MS-based metabolomics is increasingly used in the evaluation of TCMs [45,79,80]. Metabolomics seeks to elucidate the therapeutic properties and metabolism of prescription drugs and the metabolic pathways involved [81][82][83][84][85][86][87][88][89][90][91][92].…”

“…Characteristic fragmentation behaviors allow identification or tentative characterization of the chemical constituents in TCMs. Recently, chromatographic fingerprinting of the components by UHPLC-MS has become a powerful, widely-used technique to analyze TCMs, because it could systematically profile the composition of samples [45,46].…”