Application of vertical microreactor stack with polystylene microbeads to immunoassay

Ukita, Yoshiaki; Asano, Teita; Fujiwara, Kana; Matsui, Katsuhiro; Takeo, Masahiro; Negoro, Seiji; Kanie, Tomohiko; Katayama, M.; Utsumi, Yuichi

doi:10.1016/j.sna.2008.01.024

Cited by 21 publications

(11 citation statements)

References 21 publications

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“…The formula (Eq.1) which is newly defined from the principle based on the governing equation (Ukita et al 2008) in complex microchannel network is used to estimate the concentrations of the solution for six reaction chambers. The experimental results are compared with the theoretical value of C (i, N) estimated by the Eq.…”

Section: Characterization Of the Integrated Microfluidic Devicementioning

confidence: 99%

“…Each enzyme in the conjugates is able to covert hundreds of substrates into products, therefore amplifies the detectable signal and enhances the sensitivity of the assay. Several ELISA microchips are developed based on an immunoreaction on the surface of a single microchannel (Mao et al 2002) or on microbeads that are trapped in the microchannel (Ukita et al 2008). James Lee and co-workers (Lai et al 2004) present an integrated microfluidic device on a compact disk (CD) to perform ELISA for rat IgG from hybridoma cell culture, the microfluidic platform combines several microfluidic functions and each step of the ELISA process is carried out automatically by controlling the rotation speed of the CD.…”

Section: Introductionmentioning

confidence: 99%

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Development of a microplate reader compatible microfluidic chip for ELISA

Hou

Zhang

Yang

et al. 2012

Biomed Microdevices

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We report a novel microfluidic device use for sandwich enzyme-linked immunoassay assay (ELISA). The related procedures including the introduction of reagents, dilution and distribution of samples, as well as immobilization of enzyme can be readily carried out on a poly (dimethylsiloxane) (PDMS) chip. Particularly, this microfluidic chip comprising of two distinct parallel units, and has an identical dimension as a conventional microtiter plate, which offers access to the directly quantitative detection by the microplate reader. Gradient-concentration reacting solutions at six different concentrations level generated by the microfluidic channel network are simultaneously transported to 24 reaction chambers to form enzymatic products. Alkaline phosphatase (ALP), 4-methylumbelliferyl phosphate (4-MUP) and KH(2)PO(4) are used as enzyme-substrate-inhibitor model, to demonstrate the utility of the developed microchip-based enzyme inhibitor assay. Various conditions such as the surface treatment of chip channels, fluids velocities, substrate concentration, and buffer pH are investigated. The present microfluidic device for ELISA holds several advantages, for instance frugal usage of samples and reagents, less of operating time, favorably integrated configuration, ease of manipulation, and could be explored to a variety of high throughput drug screening.

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Section: Characterization Of the Integrated Microfluidic Devicementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a microplate reader compatible microfluidic chip for ELISA

Hou

Zhang

Yang

et al. 2012

Biomed Microdevices

View full text Add to dashboard Cite

show abstract

“…Chemical reaction takes place on the surface of the catalyst and hence, reaction rate is influenced by the amount of catalyst rather than the volume of reactor. (Ukita et al 2008) fabricated a vertically stacked 3D reactor structure to conduct competition enzyme-linked immunosorvent assay (ELISA). Analyte nonylphenol (NP) and enzyme-labeled alkylphenol (AP) and antibody immobilized polystyrene microbeads were mixed off-chip.…”

Section: Liquid Phase Reactormentioning

confidence: 99%

Lab-on-a-chip: a component view

2010

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Miniaturization is being increasingly applied to biological and chemical analysis processes. Lab-on-a-chip systems are direct creation of the advancement in the miniaturization of these processes. They offer a host of exciting applications in several areas including clinical diagnostics, food and environmental analysis, and drug discovery and delivery studies. This paper reviews labon-a-chip systems from their components perspective. It provides a categorization of the standard functional components found in lab-on-a-chip devices together with an overview of the latest trends and developments related to lab-on-a-chip technologies and their application in nanobiotechnology. The functional components include: injector, transporter, preparator, mixer, reactor, separator, detector, controller, and power supply. The components are represented by appropriate symbols allowing designers to present their lab-on-a-chip products in a standard manner. Definition and role of each functional component are included and complemented with examples of existing work. Through the approach presented in this paper, it is hoped that modularity and technology transfer in lab-on-achip systems can be further facilitated and their application in nanobiotechnology be expanded 1 Introduction

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“…Microfluidic mixing is a critical operation in many chemical and biological applications 7 such as chemical synthesis, [8][9][10][11][12][13] polymerization, [14][15][16] extraction, [17][18][19][20][21] DNA analysis, [22][23][24][25][26] enzyme assays, 25,[27][28][29] and protein folding. [30][31][32][33] However, mixing is a challenge at the low Reynolds numbers typical of microfluidic device operation since the fluid flow is laminar.…”

Section: Introductionmentioning

confidence: 99%

3D printed selectable dilution mixer pumps

2019

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In this paper, we demonstrate the ability to 3D print tightly integrated structures with active valves, pumps, and mixers, and we use our compact chip-to-chip interconnects [Gong et al., Lab Chip 18, 639-647 (2018)] to move bulky world-to-chip connections to separate interface chips for both post-print flushing and post-cure device operation. As example devices, we first examine 3D printed pumps, followed by two types of selectable ratio mixer pumps, a linear dilution mixer pump (LDMP) and a parallelized dilution mixer pump (PDMP), which occupy volumes of only 1:5 mm 3 and 2:6 mm 3 , respectively. The LDMP generates a selectable dilution ratio from a linear set of possibilities, while the PDMP generates a denser set of possible dilutions with a maximum dilution ratio of 1/16. The PDMP also incorporates a new 4-to-1 valve to simultaneously control 4 inlet channels. To characterize LDMP and PDMP operation and performance, we present a new, low-cost video method to directly measure the relative concentration of an absorptive dye on a pixel-by-pixel basis for each video frame. Using this method, we find that 6 periods of the active mixer that forms the core of the LDMP and PDMP are sufficient to fully mix the fluid, and that the generated concentrations track the designed dilution ratios as expected. The LDMP mixes 20 nl per 4.6 s mixer pump period, while the PDMP uses parallelized input pumps to process the same fluid volume with greater choice of dilution ratios in a 3.6 s period.

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Application of vertical microreactor stack with polystylene microbeads to immunoassay

Cited by 21 publications

References 21 publications

Development of a microplate reader compatible microfluidic chip for ELISA

Development of a microplate reader compatible microfluidic chip for ELISA

Lab-on-a-chip: a component view

3D printed selectable dilution mixer pumps

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