Application of Xylanase from Alkaliphilic Thermophilic Bacillus sp. NCIM 59 in biobleaching of bagasse pulp

Kulkarni, Neelima; Rao, Mala

doi:10.1016/0168-1656(96)01616-1

Cited by 86 publications

(40 citation statements)

References 15 publications

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“…and Staphylococcus sp. (Kulkarni and Rao 1996;Gupta et al 2000;Damiano et al 2003). The crude enzyme also had broad pH optima and stability between 6.0 and 8.0 and retained 77 and 56% of enzyme activity at pH 9 and 10, respectively.…”

Section: Enzyme Characteristicsmentioning

confidence: 94%

An alkaliphilic and xylanolytic strain of actinomycetes Kocuria sp. RM1 isolated from extremely alkaline bauxite residue sites

Krishna

Arora

Reddy

2008

World J Microbiol Biotechnol

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We have isolated and characterized a xylanolytic actinomycete strain (RM1) from the extremely alkaline bauxite residue obtained from National Aluminum Company Ltd., Damanjodi, India. The phenotypic features and complete sequence of 16S rRNA revealed that this strain belong the genus Kocuria and showed 98% sequence similarity with Kocuria aegyptia. The RM1 strain was able to grow at pH 10.5 in buffered and unbuffered media and utilize 40 different carbon substrates. The RM1 strain under optimal conditions produced extracellular xylanase at 311 U/ml. The xylanase produced by RM1 showed a wide range of temperature (30-85°C) and pH (4.5-9) tolerance by retaining 90% of its activity. This is the first report of isolation of actinomycetes, Kocuria sp., which produces high amount of xylanase, from bauxite residue and offers a new source of xylanase-producing strains.

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Section: Enzyme Characteristicsmentioning

confidence: 94%

An alkaliphilic and xylanolytic strain of actinomycetes Kocuria sp. RM1 isolated from extremely alkaline bauxite residue sites

Krishna

Arora

Reddy

2008

World J Microbiol Biotechnol

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“…Cellulase-free xylanases have a wide range of potential biotechnological applications in the pulp and paper [12,23], food and beverage [19] and agricultural industries [39]. The efficient production of fungal xylanases is dependent on a number of cultivation parameters that include the nature of the inducing substrate, culture medium composition, temperature, pH, dissolved oxygen tension and shear stress in submerged culture.…”

Section: Introductionmentioning

confidence: 99%

Effect of cultivation pH and agitation rate on growth and xylanase production by Aspergillus oryzae in spent sulphite liquor

Chipeta

Preez

Christopher³

2008

J Ind Microbiol Biotechnol

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The effects of cultivation pH and agitation rate on growth and extracellular xylanase production by Aspergillus oryzae NRRL 3485 were investigated in bioreactor cultures using spent sulphite liquor (SSL) and oats spelts xylan as respective carbon substrates. Xylanase production by this fungus was greatly affected by the culture pH, with pH 7.5 resulting in a high extracellular xylanase activity in the SSL-based medium as well as in a complex medium with xylan as carbon substrate. This effect, therefore, was not solely due to growth inhibition at the lower pH values by the acetic acid in the SSL. The xylanase activity in the SSL medium peaked at 199 U ml(-1) at pH 7.5 with a corresponding maximum specific growth rate of 0.39 h(-1). By contrast, the maximum extracellular beta-xylosidase activity pf 0.36 U ml(-1) was recorded at pH 4.0. Three low molecular weight xylanase isozymes were secreted at all pH values within the range of pH 4-8, whereas cellulase activity on both carbon substrates was negligible. Impeller tip velocities within the range of 1.56-3.12 m s(-1) had no marked effect, either on the xylanase activity, or on the maximum volumetric rate of xylanase production. These results also demonstrated that SSL constituted a suitable carbon feedstock as well as inducer for xylanase production in aerobic submerged culture by this strain of A. oryzae.

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“…NCIM 59 resulted in a 21% reduction in kappa number and a 2.5% increase in brightness (Kulkarni and Rao 1996). Silva et al (1994) reported that xylanase from Humicola sp.…”

Section: Resultsmentioning

confidence: 99%

Application of Aspergillus fumigatus Xylanase for Quality Improvement of Waste Paper Pulp

Savitha

Sadhasivam

Swaminathan

2007

Bull Environ Contam Toxicol

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Application of Xylanase from Alkaliphilic Thermophilic Bacillus sp. NCIM 59 in biobleaching of bagasse pulp

Cited by 86 publications

References 15 publications

An alkaliphilic and xylanolytic strain of actinomycetes Kocuria sp. RM1 isolated from extremely alkaline bauxite residue sites

An alkaliphilic and xylanolytic strain of actinomycetes Kocuria sp. RM1 isolated from extremely alkaline bauxite residue sites

Effect of cultivation pH and agitation rate on growth and xylanase production by Aspergillus oryzae in spent sulphite liquor

Application of Aspergillus fumigatus Xylanase for Quality Improvement of Waste Paper Pulp

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