This study evaluated the potency of zein-alginate-phosphatidylcholine
nanoparticles (NPs) on bioaccessibility/intestinal uptake of encapsulated
lycopene (LY) and lutein (LT) versus dietary absorption using simulated
digestion and human intestinal Caco-2 cells. LY-zein-alginate-PC (LYZAP)
and LT-zein-alginate-PC (LTZAP) NPs yield desired properties, which
exhibit sustained release and are suitable for oral administration.
Interestingly, co-treatment of LYZAP + LTZAP showed better release
of carotenoids instead of individual treatment at intestinal pH. Bioaccessibility,
cellular uptake, and basolateral secretion of LY and LT from NPs were
significantly enhanced than micellar carotenoids (dietary mode of
absorption). The increased absorption of carotenoids from NPs correlated
with triglyceride levels. The intestinal cell uptake of carotenoids
by nanoencapsulation may be due to endocytosis, paracellular, and
SRB-1 protein-mediated transport. Overall, LYZAP and LTZAP NPs possess
superior properties to control the release and cellular uptake of
unique or distinct carotenoids. The inclusion of alginate and phosphatidylcholine
in zein-based nanoencapsulation could be a promising strategy to improve
carotenoid bioavailability.