Application to Mammalian Tissues of the Chemiluminescent Method for Detecting Acetylcholine

Israël, Maurice; Lesbats, B.

doi:10.1111/j.1471-4159.1982.tb04727.x

Cited by 155 publications

(53 citation statements)

References 4 publications

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“…The method was basically as described by Israel & Lesbats (1982). After the addition ofTCA the samples were left on ice for at least I h, centrifuged and the supernatants transferred to clean tubes.…”

Section: Determination Of Acetylcholinementioning

confidence: 99%

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Increase in acetylcholine concentrations in the brain of ‘old’ rats following treatment with pyrithioxin (Encephabol)

Martin

Vyas

1987

British J Pharmacology

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Section: Determination Of Acetylcholinementioning

confidence: 99%

“…All traces of ether were evaporated from the aqueous phase with nitrogen. Aliquots, usually 15 pl, of the oxidised sample were processed exactly as described by Israel & Lesbats (1982). Light emission was measured using a Packard Picolite luminometer and each sample was compared with suitable standards.…”

Section: Determination Of Acetylcholinementioning

confidence: 99%

Increase in acetylcholine concentrations in the brain of ‘old’ rats following treatment with pyrithioxin (Encephabol)

Martin

Vyas

1987

British J Pharmacology

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“…The chemiluminescence procedure for measuring AcCho was used (15,(24)(25)(26). In this method, AcCho esterase hydrolyzes AcCho, the choline generated is oxidized by choline oxidase, and the H202 produced generates a light emission in the presence of luminol plus peroxidase.…”

Section: Preparation Of Presynaptic Membrane Proteoliposomesmentioning

confidence: 99%

Reconstitution of a functional synaptosomal membrane possessing the protein constituents involved in acetylcholine translocation.

Israël¹,

Lesbats²,

Morel³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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Reconstitution of a functional presynaptic membrane possessing calcium-dependent acetylcholine release properties has been achieved. The proteoliposomal membrane obtained gains its acetyicholine-releasing capabilities from presynaptic membrane proteins. At the peak of acetylcholine release, intramembrane particles became more numerous in one of the proteoliposomal membrane faces. This phenomenon resembles the intramembrane particle rearrangements found in stimulated synaptosomes. No visible structures capable of releasing acetylcholine as a result of the calcium influx were found inside the proteoliposomes. This supports the view that the release of free cytosolic acetylcholine from stimulated nerve terminals can be directly attributed to presynaptic membrane proteins. These proteins were extracted in a functional form from the synaptosomal membrane.The intramembrane particles found in the presynaptic membrane undergo important changes in the course of synaptic activity (1-8). They wvere analyzed during acetylcholine (AcCho) release from Torpedo electric organ synaptosomes stimulated with a variety of agents. The only common ultrastructural change found was the appearance of a category of large (8-to 18-nm) particles while smaller particles (5-11 nm) disappeared (6-8). The large particles can be pinched-off with the E or P faces of the membrane according to the conditions used. The hypothesis that Ca2t causes the assembly of large particles in the membrane and that these particles ensure Ca2+-dependent translocation of AcCho has been put forward by Israel et al. (6)(7)(8).At present, the view is generally accepted that there is, in the nerve terminal, a genuine cytosolic free AcCho compartment (20-50%) of total AcCho (9, 10) and that the enzyme cholineacetylase, which synthesizes it, is also located in the cytosol (11). Free AcCho decreases and is renewed in the course of stimulation of electric organ slices (9,(12)(13)(14). The decrease of free AcChp has also been observed with stimulated synaptosomes, where it was directly measured with a chemiluminescent AcCho assay (15). Cytosolic AcCho also seems to be involved at Aplysia synapses (16) or at neuromuscular junctions (17). As for the vesicular AcCho pool (bound AcCho), it is mobilized during intense stimulation in Torpedo electric organ (9) and at neuromuscular synapses (17). The possibility of direct release of free AcCho from the cytosolic compartment through a presynaptic membrane element (the operator) (13) is supported by recent experiments in which synaptosomes were depleted of their contents and filled with AcCho under controlled ionic conditions. These sacs were able to release AcCho in proportion to the Ca2t influx and the internal AcCho concentration (18). It therefore became possible to try to recoyer several essential presynaptic membrane mechanisms such as choline uptake, or AcCho release, in proteoliposomes derived from the presynaptic membrane (19-21). Until recently, proteoliposomes filled with AcCho or choline had been found to exhibi...

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“…GH level was measured in serum samples by using a GH EIA kit (American Laboratory Products, Windham, NH, USA) (10). ACh levels were examined as described by Israel and Lesbats (11), and AChE activity in the hippocampal homogenates was assayed by the Ellman method (12).…”

mentioning

confidence: 99%

Growth Hormone Releaser Attenuates β-Amyloid (1 – 42)-Induced Memory Impairment in Mice

et al. 2005

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Abstract. Accumulating evidence indicates that growth hormone (GH) might be effective at preventing the development of Alzheimer's disease. However, exogenous GH treatment has exhibited side effects for clinical application; thus supplementation with amino acids to promote the release of GH could be a possible alternative treatment. In this study, mice that were fed with a diet of GH-releasing supplements had significantly attenuated memory impairments and hippocampal changes in the acetylcholinesterase activity and acetylcholine level induced by amyloid beta protein (Aβ) (1 -42). Our results suggest that the use of GH-releasing supplement exerts beneficial effects on the memory impairment induced by Aβ (1 -42).

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Application to Mammalian Tissues of the Chemiluminescent Method for Detecting Acetylcholine

Cited by 155 publications

References 4 publications

Increase in acetylcholine concentrations in the brain of ‘old’ rats following treatment with pyrithioxin (Encephabol)

Increase in acetylcholine concentrations in the brain of ‘old’ rats following treatment with pyrithioxin (Encephabol)

Reconstitution of a functional synaptosomal membrane possessing the protein constituents involved in acetylcholine translocation.

Growth Hormone Releaser Attenuates β-Amyloid (1 – 42)-Induced Memory Impairment in Mice

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