Applications, challenges, and needs for employing synthetic biology beyond the lab

Abstract

Synthetic biology holds great promise for addressing global needs. However, most current developments are not immediately translatable to ‘outside-the-lab’ scenarios that differ from controlled laboratory settings. Challenges include enabling long-term storage stability as well as operating in resource-limited and off-the-grid scenarios using autonomous function. Here we analyze recent advances in developing synthetic biological platforms for outside-the-lab scenarios with a focus on three major application spaces: bioproduction, biosensing, and closed-loop therapeutic and probiotic delivery. Across the Perspective, we highlight recent advances, areas for further development, possibilities for future applications, and the needs for innovation at the interface of other disciplines.

How to Unravel the Key Functions of Cryptic Oomycete Elicitin Proteins and Their Role in Plant Disease

“…Even though much of the research to date has been proof-of-concept, the use of CRISPR/Cas for the targeted modification of a pathogen should open up possibilities to control plant diseases. However, we cannot underestimate the challenge of translating the results of laboratory studies on oomycetes, for example, into applications in the field [67]. We need to develop greater knowledge about genetic stability, especially in oomycetes, and, also, whether the approach of using CRISPR/Cas is feasible and cost-effective in the field.…”

Combating Infectious Diseases with Synthetic Biology

“… 5 While the advancements in synthetic biology offer robust, inexpensive, and rapid platforms for detecting and eradicating diseases, four main steps should be considered during bacteria engineering in the fight against infectious diseases. 6 …”

Ten future challenges for synthetic biology

“…The first consideration is the longevity of the biosensing system. Efforts to encapsulate or lyophilise whole‐cell or cell‐free biosensors have prolonged the lifespans of biosensors and allowed transport without a cold chain [22]. Engineering sensing motifs into naturally stress resistant biological constructs such as spores and biofilms can also enable sensing under harsh environmental conditions [23].…”