Applications of alginate biopolymer in drug delivery

Grøndahl, Lisbeth; Lawrie, Gwendolyn; Anitha, A; Shejwalkar, Aparna

doi:10.1016/b978-0-08-102680-9.00014-7

Cited by 18 publications

(17 citation statements)

References 135 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…28 Natural biomaterials like alginate can be a good choice for hydrogel synthesis as it is easily available, is costeffective, and exhibits biocompatibility. 29 Encapsulating EVs into the alginate hydrogel provides a 3D matrix without affecting the cargo of EVs. 29 Along with 2D studies, recent research focuses on the delivery of EVs through a 3D microenvironment.…”

Section: Introductionmentioning

confidence: 99%

Hydrogel-Assisted 3D Model to Investigate the Osteoinductive Potential of MC3T3-Derived Extracellular Vesicles

Holkar

Kale

Ingavle

2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Effective and rapid regeneration of bone defects often pose substantial challenges in severe accidental injuries and disabilities occurring due to diseases and/or advanced age, especially in patients having reduced tissue regeneration competence. The success of mesenchymal stromal cell (MSC)-based research strategies in improving bone regeneration was hampered not only due to the limited knowledge of therapeutic actions of MSCs but also due to difficulties as well as expenses related to cell manufacturing and time taken for ethical approvals for clinical use of living cells and engineered tissues. The recent trend indicated that there is a shift from the direct usage of MSCs toward the application of paracrine factors and extracellular vesicles (EVs) isolated from their MSC secretome in bone tissue regeneration. This shift has directed research into the development of "cell-free" therapeutics, which could be a better alternative due to its several advantages over the use of their parental MSCs. Furthermore, accumulating evidence suggested that the 3D microenvironment influences the paracrine effects of MSCs. Although the osteogenic role of EVs has been explored recently, the current study showed, for the first time, that encapsulation of EVs along with MC3T3 cells in a 3D hydrogel-assisted culture with a distinct porous microenvironment having meso and macro (0.05−200 μm) pore size distribution resulted in an improved osteogenic response in vitro. The present work was primarily focused on investigating the influence of EVs isolated under distinct priming conditions to enhance the osteogenic potential. In addition, in the current work, the osteogenic ability of different types of EVs (microvesicles and exosomes) and total EVs isolated at different time points was also examined when encapsulated with MC3T3 cells in an alginate gel. Using various biochemical assays, such as alkaline phosphatase (ALP) production and calcium secretion, it was observed that both microvesicles and exosomes collected from MC3T3 cells independently had osteogenic potential; however, their collective activity was found to be superior. We further showed that EVs induce an early osteogenic response in MC3T3 cells as indicated by ALP and calcium secretion at a much earlier time point, compared to the controls. Our data suggested that this 3D hydrogel-assisted system provides close proximity of cells and EVs, and thus, mimics the in vivo scenario, making it clinically useful for bone tissue engineering.

show abstract

Section: Introductionmentioning

confidence: 99%

Hydrogel-Assisted 3D Model to Investigate the Osteoinductive Potential of MC3T3-Derived Extracellular Vesicles

Holkar

Kale

Ingavle

2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…One alternative for the delivery of highly reactive bioactive compounds, improving their bioavailability or the protection of food surfaces, is the use of edible coatings such as alginate fibers [ 12 , 13 ]. Given their water solubility, they have been widely used in several applications in the pharmaceutical and food industries, including the delivery of hydrophilic nutraceuticals, β-carotene, antibiotic drugs, and other substances [ 14 , 15 , 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…Some of the effects of their use in foods have to do with their safety, affinity with water, changes in sensory attributes, and storage stability of different formulations [ 23 , 24 ]. In addition, they have been tested as delivery systems for bioactive compounds [ 12 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Design and Optimization of a Self-Assembling Complex Based on Microencapsulated Calcium Alginate and Glutathione (CAG) Using Response Surface Methodology

et al. 2021

View full text Add to dashboard Cite

The aim of this work was to characterize and optimize the formation of molecular complexes produced by the association of calcium alginate and reduced glutathione (GSH). The influence of varying concentrations of calcium and GSH on the production of microcapsules was analyzed using response surface methodology (RSM). The microcapsules were characterized by thermogravimetric analysis (TGA-DTG) and infrared spectroscopy (FTIR) in order to assess the hydration of the complexes, their thermal stability, and the presence of GSH within the complexes. The optimum conditions proposed by RSM to reach the maximum concentration of GSH within complexes were a 15% w/v of GSH and 1.25% w/v of CaCl2, with which a theorical concentration of 0.043 mg GSH per mg of CAG complex was reached.

show abstract

“…In order to manage the release of therapeutic usage, the aimed system of drug supply must typically activate the cellular areas [118]. Therefore, to optimise the synthesis or functioning of precursors, composition conditions of manufacture, and drug encapsulation technique, a tailored drug delivery approach must fit into the required release kinetics [119]. The medicine or other nutrient should be delivered at a controlled rate and dosage by ensuring that all factors such as size, shape, surface 8 International Journal of Polymer Science morphology, bioavailability, and biodegradability are suitable and particular to the intended spot.…”

Section: Applications Of Biopolymer Materials In Biomedical Fieldsmentioning

confidence: 99%

Additive Manufacturing of Biopolymers for Tissue Engineering and Regenerative Medicine: An Overview, Potential Applications, Advancements, and Trends

Veeman

Sai

Sureshkumar³

et al. 2021

International Journal of Polymer Science

View full text Add to dashboard Cite

As a technique of producing fabric engineering scaffolds, three-dimensional (3D) printing has tremendous possibilities. 3D printing applications are restricted to a wide range of biomaterials in the field of regenerative medicine and tissue engineering. Due to their biocompatibility, bioactiveness, and biodegradability, biopolymers such as collagen, alginate, silk fibroin, chitosan, alginate, cellulose, and starch are used in a variety of fields, including the food, biomedical, regeneration, agriculture, packaging, and pharmaceutical industries. The benefits of producing 3D-printed scaffolds are many, including the capacity to produce complicated geometries, porosity, and multicell coculture and to take growth factors into account. In particular, the additional production of biopolymers offers new options to produce 3D structures and materials with specialised patterns and properties. In the realm of tissue engineering and regenerative medicine (TERM), important progress has been accomplished; now, several state-of-the-art techniques are used to produce porous scaffolds for organ or tissue regeneration to be suited for tissue technology. Natural biopolymeric materials are often better suited for designing and manufacturing healing equipment than temporary implants and tissue regeneration materials owing to its appropriate properties and biocompatibility. The review focuses on the additive manufacturing of biopolymers with significant changes, advancements, trends, and developments in regenerative medicine and tissue engineering with potential applications.

show abstract

Applications of alginate biopolymer in drug delivery

Cited by 18 publications

References 135 publications

Hydrogel-Assisted 3D Model to Investigate the Osteoinductive Potential of MC3T3-Derived Extracellular Vesicles

Hydrogel-Assisted 3D Model to Investigate the Osteoinductive Potential of MC3T3-Derived Extracellular Vesicles

Design and Optimization of a Self-Assembling Complex Based on Microencapsulated Calcium Alginate and Glutathione (CAG) Using Response Surface Methodology

Additive Manufacturing of Biopolymers for Tissue Engineering and Regenerative Medicine: An Overview, Potential Applications, Advancements, and Trends

Contact Info

Product

Resources

About