Applications of Biomaterials in 3D Cell Culture and Contributions of 3D Cell Culture to Drug Development and Basic Biomedical Research

Park, Yujin; Huh, Kang Moo; Kang, Sun-Woong

doi:10.3390/ijms22052491

Cited by 80 publications

(66 citation statements)

References 229 publications

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“…In the present work, a two-dimensional (2D) system was chosen due to the high reproducibility of the experimental results and the ease of culture maintenance. Nevertheless, the morphology as well as the functions of cells grown as a monolayer attached to a glass or plastic surface resulted in being altered compared to those in the natural environment [ 47 , 48 ]. Despite the higher costs and technical difficulties, three-dimensional (3D) cell culture models have gained increasing interest owing to their closer resemblance to the in vivo microenvironment [ 47 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the morphology as well as the functions of cells grown as a monolayer attached to a glass or plastic surface resulted in being altered compared to those in the natural environment [ 47 , 48 ]. Despite the higher costs and technical difficulties, three-dimensional (3D) cell culture models have gained increasing interest owing to their closer resemblance to the in vivo microenvironment [ 47 , 49 ]. Furthermore, bone repair is a complex process which involves the well-orchestrated interactions between different cells and signals [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

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The Effects of Three Chlorhexidine-Based Mouthwashes on Human Osteoblast-Like SaOS-2 Cells. An In Vitro Study

Brunello

Becker

Scotti

et al. 2021

IJMS

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Several decontamination methods for removing biofilm from implant surfaces during surgical peri-implantitis treatment have been reported, including the intraoperative usage of chlorhexidine (CHX)-based antiseptics. There is a lack of information on possible adverse effects on bone healing. The study aimed to examine the impact of three CHX-based mouthwashes on osteoblast-like cells (SaOS-2) in vitro. Cells were cultured for three days in 96-well binding plates. Each well was randomly treated for either 30, 60 or 120 s with 0.05% CHX combined with 0.05% cetylpyridinium chloride (CPC), 0.1% CHX, 0.2% CHX or sterile saline (NaCl) as control. Cell viability, cytotoxicity and apoptosis were assessed at day 0, 3 and 6. Cell viability resulted in being higher in the control group at all time points. At day 0, the CHX 0.2 group showed significantly higher cytotoxicity values compared to CHX 0.1 (30 s), CHX + CPC (30 s, 60 s and 120 s) and control (60 s and 120 s), while no significant differences were identified between CHX + CPC and both CHX 0.1 and NaCl groups. All test mouthwashes were found to induce apoptosis to a lower extent compared to control. Results indicate that 0.2% CHX presented the highest cytotoxic effect. Therefore, its intraoperative use should be carefully considered.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Effects of Three Chlorhexidine-Based Mouthwashes on Human Osteoblast-Like SaOS-2 Cells. An In Vitro Study

Brunello

Becker

Scotti

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…As presented before, the development of novel 3D cultures is based on a better understanding of TME structure and interactions. To improve cellular functions, various forms of biomaterials are already available serving as a scaffold for 3D cell culture which are comprehensively summarized in recent reviews ( Gu and Mooney, 2016 ; Kamatar et al, 2020 ; Park et al, 2021 ). One method is the usage of hydrogels, which can be further divided into synthetic and natural.…”

Section: Usage Of Biomaterials As Scaffolds For 3d Culture Modelsmentioning

confidence: 99%

Precision Medicine Gains Momentum: Novel 3D Models and Stem Cell-Based Approaches in Head and Neck Cancer

Affolter

Lammert

Kern

et al. 2021

Front. Cell Dev. Biol.

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Despite the current progress in the development of new concepts of precision medicine for head and neck squamous cell carcinoma (HNSCC), in particular targeted therapies and immune checkpoint inhibition (CPI), overall survival rates have not improved during the last decades. This is, on the one hand, caused by the fact that a significant number of patients presents with late stage disease at the time of diagnosis, on the other hand HNSCC frequently develop therapeutic resistance. Distinct intratumoral and intertumoral heterogeneity is one of the strongest features in HNSCC and has hindered both the identification of specific biomarkers and the establishment of targeted therapies for this disease so far. To date, there is a paucity of reliable preclinical models, particularly those that can predict responses to immune CPI, as these models require an intact tumor microenvironment (TME). The “ideal” preclinical cancer model is supposed to take both the TME as well as tumor heterogeneity into account. Although HNSCC patients are frequently studied in clinical trials, there is a lack of reliable prognostic biomarkers allowing a better stratification of individuals who might benefit from new concepts of targeted or immunotherapeutic strategies. Emerging evidence indicates that cancer stem cells (CSCs) are highly tumorigenic. Through the process of stemness, epithelial cells acquire an invasive phenotype contributing to metastasis and recurrence. Specific markers for CSC such as CD133 and CD44 expression and ALDH activity help to identify CSC in HNSCC. For the majority of patients, allocation of treatment regimens is simply based on histological diagnosis and on tumor location and disease staging (clinical risk assessments) rather than on specific or individual tumor biology. Hence there is an urgent need for tools to stratify HNSCC patients and pave the way for personalized therapeutic options. This work reviews the current literature on novel approaches in implementing three-dimensional (3D) HNSCC in vitro and in vivo tumor models in the clinical daily routine. Stem-cell based assays will be particularly discussed. Those models are highly anticipated to serve as a preclinical prediction platform for the evaluation of stable biomarkers and for therapeutic efficacy testing.

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“…The reproduction of in vivo conditions for cell growth in 3D requires a gelatinous and flexible matrix, and BNC has adequate characteristics to serve this purpose. The mechanical strength and flexibility of BNC scaffolds compensate for the most common disadvantages of peptide hydrogel matrices used in current 3D cell culture settings [ 20 , 21 ]. Additionally, the BNC scaffolds represent an easily produced low-cost alternative to expensive peptide hydrogel matrices that remain expensive due to difficulties in their production.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay

et al. 2021

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Bacterial nanocellulose (BNC) stands out among polymers as a promising biomaterial due to its mechanical strength, hydrophilicity, biocompatibility, biodegradability, low toxicity and renewability. The use of scaffolds based on BNC for 3D cell culture has been previously demonstrated. The study exploited excellent properties of the BNC to develop an efficient and low-cost in vitro cell migration assay. The BNC scaffold was introduced into a cell culture 24 h after the SW480 cells were seeded, and cells were allowed to enter the scaffold within the next 24–48 h. The cells were stained with different fluorophores either before or after the introduction of the scaffold in the culture. Untreated cells were observed to enter the BNC scaffold in significant numbers, form clusters and retain a high viability after 48 h. To validate the assay’s usability for drug development, the treatments of SW480 cells were performed using aspirin, an agent known to reduce the migratory potential of this cell line in culture. This study demonstrates the application of BNC as a scaffold for cell migration testing as a low-cost alternative to commercial assays based on the Boyden chamber principle. The assay could be further developed for routine use in cancer research and anticancer drug development.

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Applications of Biomaterials in 3D Cell Culture and Contributions of 3D Cell Culture to Drug Development and Basic Biomedical Research

Cited by 80 publications

References 229 publications

The Effects of Three Chlorhexidine-Based Mouthwashes on Human Osteoblast-Like SaOS-2 Cells. An In Vitro Study

The Effects of Three Chlorhexidine-Based Mouthwashes on Human Osteoblast-Like SaOS-2 Cells. An In Vitro Study

Precision Medicine Gains Momentum: Novel 3D Models and Stem Cell-Based Approaches in Head and Neck Cancer

Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay

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