Applications of Bound Biopolymers in Enzymology and Immunology

Novel methods have been developed for the preparation of polymers suitable for the attachment of enzymes, or other biochemical macromolecules, to render them water insoluble. By a new irradiation process, p‐nitrostyrene has been graft copolymerized to trunk polymers, such as polypropylene. Reduction of the nitro group in the graft copolymer gives the amino derivative, which may be converted to other functional groups (isothiocyanato or diazo) or used in conjunction with bifunctional reagents in order to covalently bind enzymes to produce active, water‐insoluble conjugates. The properties of one such conjugate, in which trypsin was immobilized by covalent attachment to poly(p‐isothiocyanatostyrene‐g‐propylene) are discussed. The advantages of the present radiation grafting technique are compared with conventional methods for insolubilizing enzymes.

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Immobilized Enzymes and Proteins in Urinalysis

Boguslaski¹,

Smith²

1977

Biomedical Applications of Immobilized Enzymes and Proteins

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Applications of Bound Biopolymers in Enzymology and Immunology

Cited by 9 publications

References 352 publications

The Purification of Biologically Active Compounds by Affinity Chromatography

The Purification of Biologically Active Compounds by Affinity Chromatography

The insolubilization of trypsin by attachment to radiation graft copolymers of polypropylene

Immobilized Enzymes and Proteins in Urinalysis

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