Applications of capacitation status for litter size enhancement in various pig breeds

Kwon, Woo-Sung; Shin, Dong‐Ha; Ryu, Do-Yeal; Khatun, Amena; Rahman, Saidur; Pang, Myung-Geol

doi:10.5713/ajas.17.0760

Cited by 15 publications

(14 citation statements)

References 31 publications

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“…Recently, Kwon et al used CTC combined with H33258 staining to examine sperm capacitation status and applied it to evaluate boar fertility, showing an increased litter size in a field trial [ 32 ]. They also showed that combined H33258/CTC staining might be used to predict male fertility in various pig breeds [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Serine Protease Inhibitor SERPINE2 Reversibly Modulates Murine Sperm Capacitation

Hwu

et al. 2018

IJMS

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SERPINE2 (serpin peptidase inhibitor, clade E, member 2), predominantly expressed in the seminal vesicle, can inhibit murine sperm capacitation, suggesting its role as a sperm decapacitation factor (DF). A characteristic of DF is its ability to reverse the capacitation process. Here, we investigated whether SERPINE2 can reversibly modulate sperm capacitation. Immunocytochemical staining revealed that SERPINE2 was bound onto both capacitated and uncapacitated sperm. It reversed the increase in BSA-induced sperm protein tyrosine phosphorylation levels. The effective dose and incubation time were found to be >0.1 mg/mL and >60 min, respectively. Calcium ion levels in the capacitated sperm were reduced to a level similar to that in uncapacitated sperm after 90 min of incubation with SERPINE2. In addition, the acrosome reaction of capacitated sperm was inhibited after 90 min of incubation with SERPINE2. Oviductal sperm was readily induced to undergo the acrosome reaction using the A23187 ionophore; however, the acrosome reaction was significantly reduced after incubation with SERPINE2 for 60 and 120 min. These findings suggested that SERPINE2 prevented as well as reversed sperm capacitation in vitro. It also prevented the acrosome reaction in in vivo-capacitated sperm isolated from the oviduct. Thus, SERPINE2 could reversibly modulate murine sperm capacitation.

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Section: Discussionmentioning

confidence: 99%

Serine Protease Inhibitor SERPINE2 Reversibly Modulates Murine Sperm Capacitation

Hwu

et al. 2018

IJMS

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“…Semen samples were collected after sexual maturation and diluted as described above. AI was performed by professional technicians from Sunjin Co. twice per estrus 32 , 65 , 66 . The inseminated sows were managed at 20 ± 5 °C with air circulation, 2:1 light/dark cycle, and adequate feed for pregnant sow during the experimental period.…”

Section: Methodsmentioning

confidence: 99%

Optimization of sperm RNA processing for developmental research

Pang

Kang

Ryu

et al. 2020

Sci Rep

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Recent studies have demonstrated the significance of sperm RNA function as a transporter of important information directing the course of life. To determine the message contained in sperm RNA, it is necessary to optimize transcriptomic research tools. The current study was performed to optimize the processing of sperm RNA from sample storage to quantitative real-time PCR and assess the corresponding method with to evaluate male fertility and its representative markers, equatorin (EQTN) and peroxiredoxin (PRDX). Following successive steps of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines, several options were compared using boar spermatozoa. To evaluate the optimized procedures, the relationship between mRNA expression of EQTN and PRDX in spermatozoa and the fertility (litter size) of 20 individual boars was assessed. Unexpectedly, DNase treatment during RNA isolation had the deleterious effect by decreasing the RNA concentration by 56% and eliminating the correlation between EQTN and PRDX4 mRNA expression and male fertility. Moreover, when sperm RNA was processed using the corresponding method, the results showed the highest exon sequence expression, male fertility prediction power, and consistency. This optimized protocol for predicting male fertility can be used to study the transport of messages directing the life course from spermatozoon to offspring.

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“…The supernatant containing excess dye was discarded, and the pellet was resuspended in 100-µL PBS and 100-µL CTC solution (750-mM CTC in 5-µL buffer consisting of 20-mM Tris, 130-mM NaCl, and 5-mM cysteine at a pH of 7.4). The capacitation status was assessed using a fluorescence microscope (Nikon, Tokyo, Japan) for 100 spermatozoa per slide [30]. Incapacitated sperms were indicated by green fluorescence distributed uniformly over the entire sperm head, with or without a more robust fluorescent line at the equatorial segment.…”

Section: Capacitation and Acrosome Reactionmentioning

confidence: 99%

Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility

et al. 2021

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Background and Aim: Kacang buck sperm is cryosensitive due to the seminal plasma of semen itself. Meanwhile, bull seminal plasma contains the insulin-like growth factor-1 (IGF-1) complex, which is cryoprotective. The addition of the crude protein of Simmental bull seminal plasma increased the quality of post-thawed semen of Kacang buck. The study was conducted to determine the effects of Simmental bull seminal plasma with IGF-1 on the fertility of post-thawed Kacang buck semen. Materials and Methods: Buck semen was diluted in the following skim milk-egg yolk extender preparations: Without the addition of Simmental bull seminal plasma IGF-1 complex protein (T0); with the addition of 12-μg Simmental bull seminal plasma IGF-1 complex protein (T1); and with the addition of 24-μg Simmental bull seminal plasma IGF-1 complex protein (T2). The extended semen was packed in 0.25-mL straws and frozen. Post-thawed semen fertility was evaluated based on the following variables: Sperm motility, viability, intact plasma membrane (IPM), malondialdehyde (MDA) levels, capacitation status, and acrosome reaction. The difference in each variable among the groups was evaluated using analysis of variance, followed by Tukey's honestly significant difference test, at a 95% level of significance. Meanwhile, principal component analysis (PCA) was used to identify the principal component of semen fertility among the seven parameters. Results: The T1 group showed the highest sperm motility, viability, IPM, and percentage of incapacitated sperm and the lowest MDA levels, percentage of capacitated sperm, and acrosome reaction. PCA revealed that sperm motility had a moderate to very robust correlation with other variables and is the most crucial parameter, accounting for 80.79% of all variables. Conclusion: The IGF-1 complex in Simmental bull seminal plasma was useful for increasing the fertility of post-thawed Kacang buck semen, and sperm motility was the principal component of semen fertility.

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Applications of capacitation status for litter size enhancement in various pig breeds

Cited by 15 publications

References 31 publications

Serine Protease Inhibitor SERPINE2 Reversibly Modulates Murine Sperm Capacitation

Serine Protease Inhibitor SERPINE2 Reversibly Modulates Murine Sperm Capacitation

Optimization of sperm RNA processing for developmental research

Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility

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