Applications of cellular fatty acid analysis.

Welch, David

doi:10.1128/cmr.4.4.422-438.1991

Cited by 17 publications

(16 citation statements)

References 104 publications

(157 reference statements)

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“…Fatty acid analysis (FAA) has high discriminating power (below species level) as a result of the high degree of standardization achieved and the stability of fatty acid patterns in microorganisms. There is good congruence with RNA/DNA : DNA hybridization data (Lechevalier & Lechevalier, 1988;Sasser, 1990;Janse, 1991;Welch, 1991).…”

Section: Fatty Acid Profilingsupporting

confidence: 55%

New methods of diagnosis in plant pathology – perspectives and pitfalls ¹

Janse¹

1995

EPPO Bulletin

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As a spin‐off from fundamental molecular biological research, there has been a remarkable increase in new methods for diagnosis (i.e. detection and identification) in recent years. Because of their origin, these new methods all have in common that they use structural elements of the target organisms such as nucleic acids, lipids, fatty acids, proteins, polyamines and polysaccharides as a basis. These structural elements are either used as templates for development of so‐called probes for detection (and identification) or they are placed into man‐made patterns and used for identification/classification. The article presents the advantages and perspectives of the new methods compared with conventional ones. It may be noted that, in many studies, the specificity and reproducibility of the new methods has not been adequately treated or has even been only assumed. These features, which are closely linked with experimental and sampling error, lead to the principal pitfalls of the new methods, which are also reviewed.

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Section: Fatty Acid Profilingsupporting

confidence: 55%

New methods of diagnosis in plant pathology – perspectives and pitfalls ¹

Janse¹

1995

EPPO Bulletin

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“…As microbes have the characteristic patterns of FA and can be defined to the species level and results are achieved rapidly, FAMEs analysis can be considered a powerful tool for microbial identification [34]. On the other hand, FA profiles are related to some bacterial virulence, which can be utilized to distinguish pathogenic and avirulent bacteria.…”

Section: Discussionmentioning

confidence: 99%

Fatty acid profiles of Vibrio parahaemolyticus and its changes with environment

Wang

Mou

2013

J. Basic Microbiol.

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The fatty acid (FA) profiles of 15 strains representing four genera (Vibrio, Pseudomonas, Aeromonas, and Shewanella) and seven species were compared by capillary gas chromatography with flame ionization detection. FA fingerprints of Vibrio parahaemolyticus were established by similarity calculation and principle component analysis. This provided a simple measure for distinguishing V. parahaemolyticus from other bacteria. The similarity scores indicated by correlation coefficient and vector cosine were divided into three regions and suggested that the strains with scores higher than 0.980 probably belonged to V. parahaemolyticus. However, samples with low scores (<0.850) were classified under other genera. Furthermore, the alterations in FA profiles of V. parahaemolyticus in the presence of various environmental pressures were investigated. The production of saturated FA (SFA) increased gradually concomitant with a decreased proportion of unsaturated FA (UFA) with rising temperature. Similarly, the SFA tended to increase at the expense of UFA with prolonged culture time. In addition, V. parahaemolyticus changed its FA profiles to contain increased short-chained FA to resist an acidic environment, whereas alkaline conditions stimulated high production of long-chained FA. Analysis on FA profile is valuable for the physiological study of V. parahaemolyticus and its rapid identification.

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“…It is highly likely that the Trinidadian isolates and the pathogen as described by Prior & Rott belong to the same group. Quantitative fatty-acid analysis has been widely used in the classification and identification of bacteria (Welch, 1991), and in the characterization of phytopathogenic bacteria (Henningson & Gudmestad, 1991;Stead, 1992;Vauterin et al, 1992). In a major study involving 340 bacteria from the family Pseudomonadaceae, representing all the plant pathogenic taxa, strains were recovered in six major fatty-acid groups (Stead, 1992).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a pseudomonad‐like bacterium pathogenic for Anthurium species ¹

1995

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Bacterial isolates were obtained from diseased anthuriums at a number of locations in Trinidad. The biochemical and physiological characteristics of these strains were identical to those of a recently described group of pseudomonad‐like bacteria pathogenic on Anthurium species. These isolates were studied further using chemotaxonomic and molecular techniques to elucidate the true identity of the pathogen. Fatty‐acid profiles showed marked similarities to certain pseudomonads now placed within the genus Acidovorax. In general, major amounts of 16:1 and 16:0 were detected. Significantly, only a single hydroxy fatty acid, 10:0 3‐OH, was found in all strains. The latter characteristic is common for most members of the genera Acidovorax and Comamonas. Isolates could not be unequivocally assigned to any known taxa within these genera. Isolates were also studied by randomly amplified polymorphic DNA analysis (RAPD). Results from this simple method have confirmed the uniqueness of this group whilst establishing its similarity to the genus Acidovorax.

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Applications of cellular fatty acid analysis.

Cited by 17 publications

References 104 publications

New methods of diagnosis in plant pathology – perspectives and pitfalls ¹

New methods of diagnosis in plant pathology – perspectives and pitfalls ¹

Fatty acid profiles of Vibrio parahaemolyticus and its changes with environment

Characterization of a pseudomonad‐like bacterium pathogenic for Anthurium species ¹

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Applications of cellular fatty acid analysis.

Cited by 17 publications

References 104 publications

New methods of diagnosis in plant pathology – perspectives and pitfalls 1

New methods of diagnosis in plant pathology – perspectives and pitfalls 1

Fatty acid profiles of Vibrio parahaemolyticus and its changes with environment

Characterization of a pseudomonad‐like bacterium pathogenic for Anthurium species 1

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Product

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New methods of diagnosis in plant pathology – perspectives and pitfalls ¹

New methods of diagnosis in plant pathology – perspectives and pitfalls ¹

Characterization of a pseudomonad‐like bacterium pathogenic for Anthurium species ¹