Applications of electron microscopy in ticks: description, detection of pathogens, and control

Abdel-Shafy, Sobhy; Ghazy, Alaa A.; Shaapan, Raafat M.

doi:10.1007/s00580-018-2786-2

Cited by 5 publications

(3 citation statements)

References 56 publications

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“…because Rh. sanguineus ticks was detected as the principle vector of rickettsiae in Egypt [41,42]. Some ticksinfested animals were negative for rickettsiae in the present study.…”

Section: Primer Amplifying Sequence Of Rickettsiae Wassupporting

confidence: 54%

Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt

et al. 2019

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“…because Rh. sanguineus ticks was detected as the principle vector of rickettsiae in Egypt [41,42]. Some ticksinfested animals were negative for rickettsiae in the present study.…”

Section: Primer Amplifying Sequence Of Rickettsiae Wassupporting

confidence: 54%

Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt

et al. 2019

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“…In addition, nonengorged adult ticks from both populations were examined to record different morphometric variables, as described elsewhere for other Amblyomma species (Nava et al, 2014). Two males and two females from each site and host, were examined under a scanning electron microscope (SEM), after undergoing previous preparation protocols (Durden et al, 2018;Abdel-Shafy et al, 2019) for microscopic observation (Quorum, Model: Q150R ES).…”

Section: Methodsmentioning

confidence: 99%

Amblyomma nodosum Neumann, 1889 on collared anteaters (Tamandua tetradactyla) from the Andean region of Colombia

Robayo-Sánchez

Ramírez-Hernández

Cortés-Vecino

2020

Rev. Bras. Parasitol. Vet.

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Amblyomma nodosum is a Neotropical tick species distributed from Mexico to Argentina, with adult individuals infesting different mammal species, including anteaters (Pilosa: Myrmecophagidae). Few reports in Colombia have recorded this species in departments such as Meta, Tolima and Valle del Cauca. In this paper we describe this species using taxonomic, morphometric and molecular methods after collecting individuals parasitizing collared anteaters (Tamandua tetradactyla) in the departments of Cundinamarca and Tolima. Adult specimens were identified based on current taxonomic keys and different morphometric variables were documented in nonengorged individuals. Also, DNA was extracted for PCR amplification and sequencing of 16S rDNA, COI and ITS2 genes. Phylogenetic trees were built. One hundred and thirty-four adult ticks were collected and identified as Amblyomma nodosum, based on morphological, morphometric, molecular and phylogenetic analyses. This is the first study to report the presence of this tick species in the department of Cundinamarca, using multiple identification approaches, thus expanding its geographical records in Colombia.

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“…Therefore, this method has significantly strengthened the identification process and balanced the information attained from stereo microscopic observation (Wergin et al 2006). Recent advancement of SEM technology has been used to examine extracellular and intracellular surfaces (including cells and tissues) morphological structures of different stages of ticks (Abdel-Shafy et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Description of ticks species from Rattus tiomanicus in Mangrove Forests through scanning electron microscopy

Asyikha

Mohd-Taib

Ishak

et al. 2022

saa

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Ticks are ill-famed vectors of many pathogenic organisms which can cause various diseases and life-threatening illnesses to animals and humans. Each tick’s species and its life stages have distinct morphological features that can permit them to be accurately identified. However, the use of conventional stereo microscopes limits the accuracy of species identification. The taxonomy of ticks, in general, is not much understood and existing information is based on sparse morphology data. Thus, this study aims to examine and describe the morphological characteristics of different species of ticks collected on rodents in mangrove forests using scanning electron microscopy (SEM). This method renders high-quality images of body parts of ticks. Five different morphospecies of ticks from one host species (Rattus tiomanicus) were examined under SEM, followed by the PCR technique using mitochondrial 16S rDNA gene for species validation. This study revealed that the ticks belong to five species: Dermacentor auratus, Ixodes granulatus, Haemaphysalis hystricis, D. atrosignatus and Amblyomma cordiferum. The combination of stereomicroscopic and SEM methods has improved our understanding of the morphological characteristics of different tick species, hence establishing up-to-date taxonomic keys for these species. Moreover, due to the lack of taxonomic keys on the immature stage of ticks, the SEM method is essential in characterising the morphological features of these stages in detail, subsequently helpful in revising the taxonomic keys for certain ticks species.

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Applications of electron microscopy in ticks: description, detection of pathogens, and control

Cited by 5 publications

References 56 publications

Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt

Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt

Amblyomma nodosum Neumann, 1889 on collared anteaters (Tamandua tetradactyla) from the Andean region of Colombia

Description of ticks species from Rattus tiomanicus in Mangrove Forests through scanning electron microscopy

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