Applications of electrowetting-on-dielectric (EWOD) technology for droplet digital PCR

Rui, Xichuan; Song, Shuren; Wang, Wei; Zhou, Jia

doi:10.1063/5.0021177

Cited by 49 publications

(27 citation statements)

References 104 publications

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“…2. DMF: DMF includes manipulating and creating droplets inside microdevices [ 28 ]. Because of the dominance of molecular diffusion in continuous microfluidic flow, they manipulate and control particular regions of fluid rather than the overall fluid flow.…”

Section: Reviewmentioning

confidence: 99%

A Review of the Impact of Microfluidics Technology on Sperm Selection Technique

Olatunji¹,

More²

2022

Cureus

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Sperm sorting procedures depend on centrifugation processes. These processes produce oxidative stress and cell damage that are undesirable for in-vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) outcomes because they affect fertilization and implantation chances. The microfluidic sperm selection technique has shown promise in this area. It can create a platform for isolating and manipulating goodquality sperm cells using diverse triggers such as mechanical factors, chemical agents, and temperature gradients. Furthermore, microfluidic platforms can direct sperm cells for IVF or sperm sorting by utilizing an approach that is passive or active. In this review, we explain the use of microfluidics technologies for sorting and arranging sperm cells for different purposes. We also discuss the use of microfluidics technology in selecting and assessing sperm parameters and how it affects male infertility.

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Section: Reviewmentioning

confidence: 99%

A Review of the Impact of Microfluidics Technology on Sperm Selection Technique

Olatunji¹,

More²

2022

Cureus

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“…One of the well-known approaches for the alteration of wettability is the application of an electric field . At the micro-scale, electrowetting has found extensive usage in the fabrication of a number of lab-on-a-chip miniature devices. − Owing to the robustness and flexibility of the electrowetting-on-dielectric (EWOD)-based actuation, it has been utilized in MEMS for biomedical applications, in electrospraying, electrospinning, , inkjet printing, nanoimprinting, , manufacturing at the nanoscale, and tunable optical devices like lenses. − Electrowetting actuation enables quick and precise control of individually addressable droplets with minimal contamination and hence finds widespread use in digital microfluidics, e.g., DNA assays, lab-on-a-chip devices, , proteomics, and chemical analysis …”

Section: Introductionmentioning

confidence: 99%

“…2−4 Owing to the robustness and flexibility of the electrowetting-on-dielectric (EWOD)-based actuation, it has been utilized in MEMS for biomedical applications, in electrospraying, 5 electrospinning, 6,7 inkjet printing, 8 nanoimprinting, 9,10 manufacturing at the nanoscale, 11 and tunable optical devices like lenses. 12−14 Electrowetting actuation enables quick and precise control of individually addressable droplets with minimal contamination and hence finds widespread use in digital microfluidics, e.g., DNA assays, 4 lab-on-achip devices, 2,15 proteomics, 15 and chemical analysis. 15 It is apparent that at the nanoscale, fast and definite control of nanodroplets could play a significant role in a number of important applications.…”

Section: Introductionmentioning

confidence: 99%

Molecular Investigation of the Actuation of Electrowetted Nanodroplets

2022

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It is well known that the wettability of a droplet on a solid substrate can be modified by the application of an electric field. The phenomenon of electrowetting along with the associated physics of droplet shape change and dynamics has traditionally been studied at the micro-scale leading to exciting applications. The present work is undertaken to explore the physics of electrowetting actuation of droplet movement at the molecular level. Molecular simulations are performed to obtain the dynamic spreading of the droplet under the action of a radially symmetric electric field on a silica substrate. The dynamic behavior of the contact diameter is found to be qualitatively similar to that observed at the laboratory scale. Further simulations of droplet actuation across an array of electrodes illustrated the dynamics of the center of mass, which is then used to estimate the contact line friction and compared with the predictions from a reduced-order model. A scaling analysis is used to probe the physics of the problem correlating the contact line friction coefficient and the droplet velocity after actuation. The results and understanding elicited from the fundamental approach have the potential to guide the development of quick and precise control of nano-sized droplets and may prove to be pivotal in the development of future nanofluidic systems, nanomanufacturing methodologies, and high-resolution optoelectronic devices.

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“…The DMF device has been widely applied for biomedical applications. The advantages of DMF include minimized consumption of reagents, rapid reactions and modest cost (e.g., DNA-based applications, protein-based applications, cell-based applications and clinical applications [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]). A review of analogue and digital microfluidics is shown in Figure 1 [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

A Review on Microfluidics: An Aid to Assisted Reproductive Technology

2021

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Infertility is a state of the male or female reproductive system that is defined as the failure to achieve pregnancy even after 12 or more months of regular unprotected sexual intercourse. Assisted reproductive technology (ART) plays a crucial role in addressing infertility. Various ART are now available for infertile couples. Fertilization in vitro (IVF), intracytoplasmic sperm injection (ICSI) and intrauterine insemination (IUI) are the most common techniques in this regard. Various microfluidic technologies can incorporate various ART procedures such as embryo and gamete (sperm and oocyte) analysis, sorting, manipulation, culture and monitoring. Hence, this review intends to summarize the current knowledge about the application of this approach towards cell biology to enhance ART.

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Applications of electrowetting-on-dielectric (EWOD) technology for droplet digital PCR

Cited by 49 publications

References 104 publications

A Review of the Impact of Microfluidics Technology on Sperm Selection Technique

A Review of the Impact of Microfluidics Technology on Sperm Selection Technique

Molecular Investigation of the Actuation of Electrowetted Nanodroplets

A Review on Microfluidics: An Aid to Assisted Reproductive Technology

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