Applications of Fish Cell Cultures

Aarattuthodiyil, Suja; Dharan, Vandana

doi:10.1007/978-981-16-5195-3_7

Cited by 3 publications

(3 citation statements)

References 221 publications

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“…The surge in the use of sh cell culture in research is mainly due to the demand to reduce or replace animal experiments. Additionally, sh cell culture allows drugs to be tested, is reproducible and economically feasible, and yields result rapidly (Aarattuthodi et al 2021). Fish cell culture also functions as the best source for animal-free seafood production.…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of satellite muscle cells from olive flounder (Paralichthys olivaceus): Isolation, culture conditions, and myogenic protein expression

Krishnan

Ulagesan

Cadangin

et al. 2023

Preprint

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Olive flounder (Paralichthys olivaceus) muscle satellite cells (OFMCs) were obtained by enzymatic primary cell isolation and the explant method. Enzymatic isolation yielded cells that reached 80% confluence within 8 days, compared to 15 days for the explant method. Optimum OFMC growth was observed in 20% fetal bovine serum at 28℃ with NaCl2 and CaCl2, as well as an essential fibroblast growth factor. Olive flounder skeletal muscle myoblasts were induced into a mitogen-poor medium containing 2% horse serum for differentiation; they fused to form multinucleate myotubes. The results indicated complete differentiation of myoblasts into myotubes; we also detected the expression of the myogenic regulatory factors myoD, myogenin, and desmin. Upregulation (Myogenin, desmin) and downregulation (MyoD) of muscle regulation factors confirmed the differentiation in OFMC.

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Section: Introductionmentioning

confidence: 99%

Establishment and characterization of satellite muscle cells from olive flounder (Paralichthys olivaceus): Isolation, culture conditions, and myogenic protein expression

Krishnan

Ulagesan

Cadangin

et al. 2023

Preprint

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“…Fish-derived cell has become an emerging model in studying various aspects of science. Information and scientific data obtained from the cell culture system can be used to get insight into various biological process such as reproduction, growth, disease, [1,2] and provide tool in many research fields including immunology, biotechnology, ecotoxicology [3]. In addition to its wide range of usage, cell culture getting more preferences because cells can be genetically manipulated, quantified, and characterized using molecular techniques and can be cryopreserved and revived for future applications [4].…”

Section: Introductionmentioning

confidence: 99%

“…Now days there are a number of fish-derived cell have been developed and commercially available. There are 13 fish cell line derived from muscle tissue of 11 species of teleost [6], and at least 30 fish cell lines listed in American Type Culture Collection (ATCC), and European Collection of Authenticated Cell Cultures (ECACC) [3]. Amongst those fish cell lines, there is a kidney derived cell line i.e.…”

Section: Introductionmentioning

confidence: 99%

The primary culture and subculture of kidney-derived cells of the bony lip barb fish (Osteochilus vittatus) using different concentrations of serum

Firdauzi

Wijayanti

Habibah

2023

Proceeding ICMA-SURE

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Cell and tissue culture have advanced and developed in a short time yet continuous improvement needs to be conducted for better results. This research was conducted to obtain suitable culture conditions of kidney-derived cell culture from bony lip barb kidney (Osteochilus vittatus). The development of primary cell culture from the kidney fragments was performed in a cell culture medium composed of DMEM supplemented with various concentrations (0%, 5%, 10%, and 15%) of Fetal Bovine Serum, Penicillin-Streptomycin, and 2Mm L-Glutamine, and incubated at 29°C with 0.8% CO2. The results showed that the primary cell culture achieved confluence on day four and subsequently subcultured. The addition of 10% serum increased cell density and cell survival. The cell morphological evaluation using a phase-contrast microscope showed two dominant cell types for both primary and subcultures, i.e. erythrocytes and small-rounded cells. The highest cell yield was obtained with the addition of 10% serum concentration with Population Doubling Time (PDT) and Population Doubling Level (PDL) for 69 h 23 min and 0.47 times, respectively. Statistical analysis indicated that serum addition significantly increased cell density of the primary culture (p<0.05). A better proportion of serum supplementation to culturing success is essential to establish an auspicious cell line.

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Algal protein-based 3D-printed fish-analogs as a new approach for sustainable seafood

Alasibi,

Kazir,

Israel

et al. 2024

Current Research in Food Science

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Applications of Fish Cell Cultures

Cited by 3 publications

References 221 publications

Establishment and characterization of satellite muscle cells from olive flounder (Paralichthys olivaceus): Isolation, culture conditions, and myogenic protein expression

Establishment and characterization of satellite muscle cells from olive flounder (Paralichthys olivaceus): Isolation, culture conditions, and myogenic protein expression

The primary culture and subculture of kidney-derived cells of the bony lip barb fish (Osteochilus vittatus) using different concentrations of serum

Algal protein-based 3D-printed fish-analogs as a new approach for sustainable seafood

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