Applications of flow cytometry for the detection and characterization of biological aerosols

Sincock, Stephanie A.; Kulaga, Henrietta; Cain, Michael D.; Anderson, Patricia F; Stopa, Peter J.

doi:10.1002/(sici)1520-6521(1999)3:4/5<291::aid-fact8>3.0.co;2-5

Cited by 15 publications

(9 citation statements)

References 18 publications

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“…The instrument was calibrated with DNA‐Check beads (Coulter) using a protocol supplied by the manufacturer. The analysis protocol was based on previous work (11). The instrument was triggered on side scatter (SS) and photomultiplier voltages were set empirically so as to give a large separation between the reactants and products of the immunoassay reaction.…”

Section: Methodsmentioning

confidence: 99%

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The flow cytometry ofBacillus anthracis spores revisited

Stopa

2000

Cytometry

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Section: Methodsmentioning

confidence: 99%

“…In this laboratory, immunological analysis on the flow cytometer was successfully used to detect the spores of Bacillus subtilis var. niger and the vegetative cells of Pantoea agglomerans (formerly known as Erwinia herbicola ) directly in aerosol samples with a relatively simple protocol (11).…”

mentioning

confidence: 99%

The flow cytometry ofBacillus anthracis spores revisited

Stopa

2000

Cytometry

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“…Among the papers that report successful applications of flow cytometry to real-time investigation of biological aerosol are those by Sincock et al (1999), Chen and Li (2005) and Chen and Li (2007). Fluorescent in situ hybridization (FISH) flow cytometry has been used by a number of groups to characterise airborne bacteria and aerosolised byproducts (e.g.…”

Section: Flow Cytometrymentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

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A B S T R A C T Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP.

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“…Flow cytometry (FCM) is useful for the rapid identification and quantification of bacteria in both aquatic and air environments (Day et al 2002;Lange 232 P.-S. CHEN AND C.-S. LI et al 1997;Monfort and Baleux 1992;Sincock et al 1999). When used with a variety of dye stains, FCM could also provide a much more rapid and accurate viability assay than EFM (Button et al 2001;Deleo et al 1996;Gérald Grégori et al 2001;Henningsen et al 1997;Lopez-Amoros et al 1995;Sieracki et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Sampling Performance for Bioaerosols by Flow Cytometry with Fluorochrome

Chen

2005

Aerosol Science and Technology

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Although culture-based analysis remains the primary method for environmental bioaerosol analysis, for better understanding and quantifying of bioaerosols both culture-and nonculture-based methods should be used and compared. Here, flow cytometry with fluorochrome (FCM/FL) was applied to evaluate the sampling performance of impingement (AGI-30 all-glass impinger) and filtration (track-etched polycarbonate filter) with different types of fluorescent dye staining (cell membrane integrity and metabolism) and then compared with a traditional culture method (culturability). Two bacterial aerosols (Escherichia coli and endospores of Bacillus subtilis) and two fungal aerosols (Candida famata and Penicillium citrinum spores) were studied. The bioaerosol viability during the sampling processes was highly influenced by bioaerosol characteristics (hardy or fragile), as well as by the fluorescent dyes with different physiological mechanisms. For better viability of the sampled bioaerosol, the impinger was superior to the filter. Moreover, it was found that sampling stress from filtration had more influence on the bioaerosol metabolism mechanism than cell membrane integrity. Furthermore, the differences between cell membrane integrity and the metabolism by sampling stress were found to be related to the bioaerosol species.

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Applications of flow cytometry for the detection and characterization of biological aerosols

Cited by 15 publications

References 18 publications

The flow cytometry ofBacillus anthracis spores revisited

The flow cytometry ofBacillus anthracis spores revisited

Primary biological aerosol particles in the atmosphere: a review

Sampling Performance for Bioaerosols by Flow Cytometry with Fluorochrome

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