Applications of Genomics, Metabolomics, Fourier Transform Infrared in the Evaluation of Spoilage Targets of Shewanella putrefaciens from Spoiled Bigeye Tuna

Abstract

Shewanella putrefaciens is a typical spoiler that is commonly found in seafood and has high spoilage potential. However, the spoilage mechanism against Shewanella putrefaciens at the gene and metabolism levels has not been well elucidated. This work determined the spoilage targets on Shewanella putrefaciens XY07 from spoiled bigeye tuna by genome sequencing, metabolomics, and Fourier transform infrared (FTIR) analysis. Shewanella putrefaciens XY07 contained some genes on spoilage regulating of cys genes, his genes, spe genes and rpoS gene involved in sulfur metabolism, histidine metabolism, arginine and proline degradation, and biofilm formation at the genome level, respectively. Some spoilage genes like speC, cysM, trxB genes were identified. In addition, ABC transporters, arginine and proline metabolism; beta-alanine metabolism; glycine, serine, and threonine metabolism; histidine metabolism; sulfur metabolism; and lipid metabolism were identified as important pathways related to aquatic food during spoilage, which indicated the functions of amino acid degradation in S. putrefaciens XY 07 by metabolomics analysis. The metabolites of l-ornithine, 5-aminopentanoate, and 4-aminobutyraldehyde could be further metabolized to spermidine and spermine, producing a spoilage odor, and were involved in arginine and proline metabolism serving as key spoilage regulating metabolisms. Therefore, Shewanella putrefaciens XY07 was applied to genomics, metabolomics analysis, and FTIR to provide comprehensive insight into the investigation of spoilage targets.

Coculture of Acinetobacter johnsonii and Shewanella putrefaciens Contributes to the ABC Transporter that Impacts Cold Adaption in the Aquatic Food Storage Environment

“…The number of Shewanella putrefaciens and the level of some biochemical indexes are used to evaluate the freshness of aquatic food in a cold storage environment. 8,9 S. putrefaciens has been reported to be isolated from various aquatic species, including bigeye tuna, 10 channel catfish, 11 large yellow croaker, 12 silver European eel fish, 13 and rainbow trout (Oncorhynchus mykiss). 14 Some studies have demonstrated that S. putrefaciens has a strong spoilage ability to degrade nitrogenous substances, proteins, and amino acids through the regulation of quorum sensing, lipid metabolism, and amino acid metabolism related gene expression during cold storage.…”

“…The number of Shewanella putrefaciens and the level of some biochemical indexes are used to evaluate the freshness of aquatic food in a cold storage environment. , S. putrefaciens has been reported to be isolated from various aquatic species, including bigeye tuna, channel catfish, large yellow croaker, silver European eel fish, and rainbow trout (Oncorhynchus mykiss) . Some studies have demonstrated that S.…”