Applications of high-performance liquid chromatography in the pharmaceutical industry

Bailey, F.

doi:10.1016/s0021-9673(00)82237-2

Cited by 22 publications

(6 citation statements)

References 75 publications

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“…High Performance Liquid Chromatography (HPLC) is a chromatographic technique used in the fields of analytical chemistry, biochemistry and industrial. The applicability of HPLC for the analysis, separation and determination of alkaloids, antibiotics, nitrogen-containing compounds (nucleic acids), steroids, sulphur containing compounds, antioxidants, carcinogens and preservatives in pharmaceutical analysis 16 HPLC has many applications in both laboratory and clinical science. It is a common technique used in pharmaceutical development, as it is a dependable way to obtain and ensure product purity.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Screening, GC-MS & HPLC Analysis of Decalepis arayalpathra (Joseph & Chandras) an Endangered Medicinal Plant

Ashalatha

Raveesha

2022

Int J Life Sci Pharm Res

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Decalepis arayalpathra is one of the important medicinal plants with milky latex, which grows on the exposed rocky slopes at an 800-1200 m elevation. It is endemic to the southern region of the Western Ghats chiefly in the Agasthyamalai region of Tamil Nadu. The plant has been used by traditional people to treat various diseases such as peptic ulcers, cancer like affliction, skin diseases etc. The moniliform tuberous roots have special fragrance like vanilla, because the roots contain different compounds such as α-amyrin acetate, ß-sitosterol, naringenin, kaempferol and aromadendrin especially in 2-hydroxy 4-methoxy benzaldehyde (2H4MB). The present study was aimed to examine the phytochemical screening and identification of bioactive compounds of Decalepis arayalpathra by GC-MS & HPLC analysis. The phytochemical analysis was carried out by using standard methods. Preliminary phytochemical screening showed major bioactive compounds such as alkaloids, flavonoids, proteins, steroids, terpenoids, quinones, resins, phenols, tannins, volatile oils and other phytocompounds. FTIR analysis identified various bioactive components and those were confirmed through GC-MS analysis. 2-hydroxy 4-methoxybenzaldehyde (2H4MB) and ß-sitosterol were quantified by HPLC analysis. Further studies have to be carried out in the in-vitro multiplication, isolation and characterization of compounds to enhance the root flavouring complexes for medicinal, food and beverage industries.

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Section: Introductionmentioning

confidence: 99%

Phytochemical Screening, GC-MS & HPLC Analysis of Decalepis arayalpathra (Joseph & Chandras) an Endangered Medicinal Plant

Ashalatha

Raveesha

2022

Int J Life Sci Pharm Res

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“…In summary, this method is selective, sensitive, rapid, and reproducible for ganciclovir in its tablets. The precision and accuracy are within acceptable ranges, the quantification limit is as low as 20 ng mL −1 [27], and finally the assay is robust and rugged. permission and facilities.…”

Section: Discussionmentioning

confidence: 87%

“…Besides, short retention time (3.117), runtime (10 min), and flow rate (1 mL min −1 ) make the method attractive since these features help in saving cost and time of analysis. Ammonium acetate has many properties which are particularly interesting for use on a general purpose buffer [27] the most significant being low toxicity and cost, easy availability, good buffering capacity in the chosen pH range, and ability to provide greatly improved separations without column deterioration. In summary, this method is selective, sensitive, rapid, and reproducible for ganciclovir in its tablets.…”

Section: Discussionmentioning

confidence: 99%

Development and Validation of RP-HPLC Method for the Determination of Ganciclovir in Bulk Drug and in Formulations

Ramesh

Basavaiah

Vinay

et al. 2012

ISRN Chromatography

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A simple, rapid, accurate, and precise gradient reversed-phase HPLC (RP-HPLC) method has been developed for the determination of ganciclovir (GNC) in pharmaceuticals. Chromatographic separation was carried out on inertsil ODS C18 (4.6 mm mm, 5.0 μm) LC column using ammonium acetate buffer, sodium salt of hexane sulfonic acid as ion-pairing reagent in 1000 mL water, and acetonitrile (90 : 10) (v/v) as mobile phase at a flow rate of 1.0 mL and with UV detection at 245 nm at column temperature (30°C). The runtime under these chromatographic conditions was 10 min. The method was linear over the range of 0.02–75 μg . The limits of detection (LOD) and quantification (LOQ) values were 4.1 and 20 ng , respectively. The method was successfully extended to study the effect on GNC upon treatment with 2 N NaOH, 2N HCl, and 5% H2O2 for 2 hrs at 80°C and upon exposure to UV (1200 K lux hrs) for 72 hrs and thermal (105°C) for 5 hrs. The proposed method was further applied to the determination of GNC in pharmaceuticals, with good percent recovery. The accuracy and the precision of the method were validated on intraday and interday basis in accordance with ICH guidelines.

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“…Recent reviews (254)(255)(256)(257)(258)(259)(260)(261)(262)(263)(264) summarized advances in this area, and several examples of these techniques have been included in this review. The latter two techniques not only offer separation but provide precise methods of quantitation.…”

Section: Stability-indicating Methodsmentioning

confidence: 99%

Stability of Pharmaceuticals

Mollica¹,

Ahuja²,

Cohen³

1978

Journal of Pharmaceutical Sciences

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Applications of high-performance liquid chromatography in the pharmaceutical industry

Cited by 22 publications

References 75 publications

Phytochemical Screening, GC-MS & HPLC Analysis of Decalepis arayalpathra (Joseph & Chandras) an Endangered Medicinal Plant

Phytochemical Screening, GC-MS & HPLC Analysis of Decalepis arayalpathra (Joseph & Chandras) an Endangered Medicinal Plant

Development and Validation of RP-HPLC Method for the Determination of Ganciclovir in Bulk Drug and in Formulations

Stability of Pharmaceuticals

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