APPLICATIONS OF ISSR MARKERS IN STUDIES OF GENETIC DIVERSITY OF Pityrocarpa moniliformis

Abstract

Pityrocarpa moniliformis (Benth.) Luckow & R. W. Jobson (Fabaceae) is a native brazilian species with high potential for economic development programs in semiarid regions, mainly related to the production of honey, animal food and firewood. Thus, the objective of this work was to select Inter-Simple Sequence Repeat (ISSR) molecular markers for genetic diversity studies, as well as to test the efficiency of this approach in quantifying the genetic diversity of a natural P. moniliformis population. For this, 28 ISSR molecular markers were tested, evaluating the total number of loci, polymorphism rate and the Polymorphism Information Content (PIC) for the selected primers, the “Marker Index”, and the “Resolving Power”. Genetic diversity parameters (Nei genetic distance and Shannon index) were evaluated for 30 individuals located in Macaíba, Rio Grande do Norte State, Brazil. Seven primers were selected, which provided 74 loci, with 82% being polymorphic, while the PIC value was 0.344. The Nei genetic distance was 0.244, and the Shannon index was 0.374. Therefore, ISSR molecular markers (UBC 827, 840, 844, 857, 859, 860 and 873) are considered efficient in studying the genetic diversity of populations for the selection of matrices and germplasm banks, and may contribute to the conservation and genetic improvement of P. moniliformis populations.

SELECTION OF ISSR MOLECULAR PRIMERS FOR STUDIES OF GENETIC DIVERSITY IN Handroanthus impetiginosus (MART. EX DC.) MATTOS

“…(65.88%) (SOUZA et al, 2018) and Pityrocarpa moniliformis (Benth.) Luckow & R. W. Jobson (82%) (FELIX et al, 2020). However, when compared to a species of the same family (Bignoniaceae), the same result was obtained for Oroxylum indicum (L.) Vent.…”

Genetic diversity of rosewood (Dalbergia latifolia) in Yogyakarta, Indonesia for plus trees selection

“…The average genetic diversity (H) of rosewood candidate trees plus in Yogyakarta is high compared to Adesmia bijuga with a value of 0.22 and a Shannon Information Index of 0.36 (Guerra et al 2018). The diversity level of the plus tree in Kulon Progo with He=0.29 is comparable to that of Pityrocarpa moniliformis with He values of He=0.24 (Felix et al 2020) and D. sissoo with He=0.21 (Dobhal et al 2019) and Caragana microphylla (He=0.16 and I=0.24) (Huang et al 2016). That level of genetic diversity is still higher than those of Hedysarum sangilense with He=0.105 (Selyutina et al 2021), Parkia biglobosa with He=0.18 (Lompo et al 2017).…”

Diversidade genética entre etnovariedades de mandioca por meio de marcadores ISSR

“…Sequence Repeats), que são marcadores que apresentam carater dominante, baixo custo, alto índice de polimorfismo e alta reprodutibilidade (CHEN et al, 2017). Além disso, os marcadores moleculares ISSR podem ser utilizados para varias espécies vegetais, sem a necessidade de um conhecimento prévio do sequenciamento do genoma (TURCHETTO et al, 2017;FELIX et al, 2020).…”