Applications of LC-MS in the study of the uptake, distribution, metabolism and excretion of bioactive polyphenols from dietary supplements

Neuropharmacology of the Naturally Occurring κ-Opioid Hallucinogen Salvinorin A

“…Several investigations explored the pharmacokinetic profile of salvinorin A, including several LC-MS studies that identified samples of salvinorin A found in plasma samples (Chavkin et al, 2004;Schmidt et al, 2005b;Barnes et al, 2006). One study (Chavkin et al, 2004) reported the identification of [Mϩ23] precursor-related ions using electrospray ionization-high-resolution MS, whereas a later report identified two major product ions (m/z 295 and 373, [MϪ60] and [MϪ138], respectively) produced by collision-induced dissociation (Barnes et al, 2006).…”

“…One study (Chavkin et al, 2004) reported the identification of [Mϩ23] precursor-related ions using electrospray ionization-high-resolution MS, whereas a later report identified two major product ions (m/z 295 and 373, [MϪ60] and [MϪ138], respectively) produced by collision-induced dissociation (Barnes et al, 2006). Schmidt et al (2005b) presented a method that demonstrated sensitivity 3 orders of magnitude greater than previously reported HPLC methods for the identification of salvinorin A in plants (Gruber et al, 1999).…”

Screening and analyzing the potential bioactive components from Shaofu Zhuyu decoction, using human umbilical vein endothelial cell extraction and high‐performance liquid chromatography coupled with mass spectrometry

“…The effects may be different when a compound is in a complex system. MS technology is widely used to identify compounds from complex chemical components (Barnes et al, 2006;Xu et al, 2008;Lu et al, 2006). However, the HUVEC extract and HPLC-MS analysis system as a screening method is a new approach, and it is the first time that this method has been applied for screening and analyzing the active components protecting against vascular endothelial (VE) cell injury from SFZYD.…”

Proteomics in ocular fluids

“…Similar to plasma, tear fluid and aqueous humor have a relatively high concentration of inorganic salts [59]. Therefore well chosen sample preparation devices for desalting, like centrifugal filters [60], dialyses, or membrane preconcentrators [61], need to be used. Furthermore, tears must be handled with special care since it could be observed that they react to changes in buffer temperatures and even incubation times.…”