Applications of Multiphoton Microscopy in Dermatology

So, Peter T. C.; Yew, Elijah Y. S.; Rowlands, Christopher J.

doi:10.1016/b978-0-12-802838-4.00019-4

Cited by 3 publications

(1 citation statement)

References 238 publications

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“…In dermatological clinical research, in vivo multiphoton microscopy 1,2 offers the possibility to avoid invasive biopsies and to supply information on the skin state before, during and after a cutaneous treatment. Epidermis and superficial dermis can be characterized with sub‐micrometer resolution up to ~160‐200 µm depth, by taking advantage of intrinsic multiphoton signals: second‐harmonic generation (SHG) created by fibrillar collagens and two‐photon excited fluorescence (2PEF) emitted by keratin, nicotinamide adenine dinucleotide, flavin adenine dinucleotide, melanin, or elastin 3 …”

Section: Introductionmentioning

confidence: 99%

In vivo multiphoton imaging for non‐invasive time course assessment of retinoids effects on human skin

Tancrède‐Bohin

Baldeweck

Brizion

et al. 2020

Skin Research and Technology

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Section: Introductionmentioning

confidence: 99%

In vivo multiphoton imaging for non‐invasive time course assessment of retinoids effects on human skin

Tancrède‐Bohin

Baldeweck

Brizion

et al. 2020

Skin Research and Technology

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In vivo multiphoton multiparametric 3D quantification of human skin aging on forearm and face

Pena

Baldeweck

Decencière

et al. 2022

Sci Rep

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Quantifying skin aging changes and characterizing its 3D structure and function in a non-invasive way is still a challenging area of research, constantly evolving with the development of imaging methods and image analysis tools. In vivo multiphoton imaging offers means to assess skin constituents in 3D, however prior skin aging studies mostly focused on 2D analyses of dermal fibers through their signals’ intensities or densities. In this work, we designed and implemented multiphoton multiparametric 3D quantification tools for in vivo human skin pigmentation and aging characterization. We first demonstrated that despite the limited field of view of the technic, investigation of 2 regions of interest (ROIs) per zone per volunteer is a good compromise in assessing 3D skin constituents in both epidermis and superficial dermis. We then characterized skin aging on different UV exposed areas—ventral and dorsal forearms, face. The three major facts of aging that are epidermal atrophy, the dermal–epidermal junction (DEJ) flattening and dermal elastosis can be non-invasively quantified and compared. Epidermal morphological changes occur late and were only objectified between extreme age groups. Melanin accumulation in suprabasal layers with age and chronic exposure on ventral and dorsal forearms is less known and appears earlier. Superficial dermal aging changes are mainly elastin density increase, with no obvious change in collagen density, reflected by SHGto2PEF ratio and SAAID index decrease and ImbrN index increase on all skin areas. Analysis of the z-dermal distribution of these parameters highlighted the 2nd 20 µm thickness normalized dermal sub-layer, that follows the DEJ shape, as exhibiting the highest aging differences. Moreover, the 3D ImbrN index allows refining the share of photoaging in global aging on face and the 3D SAAID index on forearm, which elastin or fibrillar collagens densities alone do not allow. Photoaging of the temple area evolves as a function of chronic exposure with a more pronounced increase in elastin density, also structurally modified from thin and straight elastic fibers in young volunteers to dense and compact pattern in older ones. More generally, multiphoton multiparametric 3D skin quantification offers rich spatial information of interest in assessing normal human skin condition and its pathological, external environment or product induced changes.

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Endoscopic MPM objective designed for depth scanning

Brandt

Liu

et al. 2022

Endoscopic Microscopy XVII

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Applications of Multiphoton Microscopy in Dermatology

Cited by 3 publications

References 238 publications

In vivo multiphoton imaging for non‐invasive time course assessment of retinoids effects on human skin

In vivo multiphoton imaging for non‐invasive time course assessment of retinoids effects on human skin

In vivo multiphoton multiparametric 3D quantification of human skin aging on forearm and face

Endoscopic MPM objective designed for depth scanning

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