Endoscopic MPM objective designed for depth scanning

Brandt, Christoph; Wu, Wentao; Liu, Qihao; Tang, Shuo

doi:10.1117/12.2609008

Cited by 6 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A MEMS mirror is employed for XY scanning. The depth-resolved scanning is realized by an SMA-actuated objective with a focal length of fOBJ = 3.6 mm and an NA of 0.53 [5,14].…”

Section: Methodsmentioning

confidence: 99%

Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Brandt

Tang

2023

Multiphoton Microscopy in the Biomedical Sciences XXIII

View full text Add to dashboard Cite

We report on a simultaneous 2-photon and 3-photon signal acquisition method for label-free multiphoton microscopy. With dual excitation wavelengths of 1580 nm and 790 nm and a set of filter designs, multiple contrasts including second harmonic generation, third harmonic generation, and two-photon-excitation-fluorescence images are detected and separated. The spectrum of the nonlinear signals is measured and verified by a spectrometer. Depth-resolved multimodal images are demonstrated on a silicon photonic chip and leaf samples. The simultaneous 2-and 3-photon microscopy is shown to have great potential for label-free in-vivo imaging.

show abstract

“…A MEMS mirror is employed for XY scanning. The depth-resolved scanning is realized by an SMA-actuated objective with a focal length of fOBJ = 3.6 mm and an NA of 0.53 [5,14].…”

Section: Methodsmentioning

confidence: 99%

Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Brandt

Tang

2023

Multiphoton Microscopy in the Biomedical Sciences XXIII

View full text Add to dashboard Cite

show abstract

Harmonic Generation and Impact of Phase Matching in Multimodal Multiphoton Microscopy

Tang

2023

IEEE J. Select. Topics Quantum Electron.

View full text Add to dashboard Cite

Dual-wavelength multimodal multiphoton microscope with SMA-based depth scanning

Liu

Brandt

et al. 2022

Biomed. Opt. Express

View full text Add to dashboard Cite

We report on a multimodal multiphoton microscopy (MPM) system with depth scanning. The multimodal capability is realized by an Er-doped femtosecond fiber laser with dual output wavelengths of 1580 nm and 790 nm that are responsible for three-photon and two-photon excitation, respectively. A shape-memory-alloy (SMA) actuated miniaturized objective enables the depth scanning capability. Image stacks combined with two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and third harmonic generation (THG) signals have been acquired from animal, fungus, and plant tissue samples with a maximum depth range over 200 µm.

show abstract

Endoscopic MPM objective designed for depth scanning

Cited by 6 publications

References 13 publications

Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Harmonic Generation and Impact of Phase Matching in Multimodal Multiphoton Microscopy

Dual-wavelength multimodal multiphoton microscope with SMA-based depth scanning

Contact Info

Product

Resources

About