Harmonic Generation and Impact of Phase Matching in Multimodal Multiphoton Microscopy

Wu, Wentao; Tang, Shuo

doi:10.1109/jstqe.2023.3234056

Cited by 3 publications

(1 citation statement)

References 43 publications

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“…lipid and aqueous medium interface). With signals generated from different modalities, multimodal MPM can provide complementary information representing different tissue structures and form information-rich images which are essential for label-free imaging [5][6][7][8][9]. However, label-free 2-and 3-photon imaging are typically acquired sequentially in the past due to several obstacles to simultaneous acquisition.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Brandt

Tang

2023

Multiphoton Microscopy in the Biomedical Sciences XXIII

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We report on a simultaneous 2-photon and 3-photon signal acquisition method for label-free multiphoton microscopy. With dual excitation wavelengths of 1580 nm and 790 nm and a set of filter designs, multiple contrasts including second harmonic generation, third harmonic generation, and two-photon-excitation-fluorescence images are detected and separated. The spectrum of the nonlinear signals is measured and verified by a spectrometer. Depth-resolved multimodal images are demonstrated on a silicon photonic chip and leaf samples. The simultaneous 2-and 3-photon microscopy is shown to have great potential for label-free in-vivo imaging.

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Section: Introductionmentioning

confidence: 99%

Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Brandt

Tang

2023

Multiphoton Microscopy in the Biomedical Sciences XXIII

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Layered heterogeneous structures integrated device for multiplication, division arithmetic unit and multiple-physical sensing

Xu,

Zhang,

Tang

et al. 2024

Physics of Fluids

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A layered heterogeneous structure (LHS), consisting of silver, liquid crystal, and nonlinear dielectric layers, is proposed to realize functions of computing and sensing. By leveraging the optical Tamm state, the intrinsic absorption principle of liquid crystal, and nonlinear effects, the design of an integrated device capable of passive multiplication and division operations, along with high-performance multi-physical quantity sensing functionalities, is achieved. The given LHS exhibits Janus properties, with different physical functions manifested depending on the direction of electromagnetic wave (EW) propagation. During forward propagation of EWs, the LHS displays high and sharp absorptivity peaks at 774.8 and 1517.6 nm. The relationship between the two peaks approximates a frequency multiplication factor of 1.960, enabling signal multiplication. Furthermore, the two absorptivity peaks at different wavelengths facilitate the sensing of serum creatinine solution concentration and external pressure, with sensitivity (S), quality factors (Q), and figure of merit (FOM) of 266.76 μmol L−1/nm and 213.33 GPa/nm, as well as 248.76 and 348.22, 84.1 L (μmol)−1 and 49.06 GPa−1, respectively. During backward propagation of EWs, absorptivity peaks with distinct resolutions are observed at 1423 and 2809 nm, with a multiple relationship between them of 1.974, enabling frequency doubling for signal division. Additionally, the absorptivity curve facilitates temperature sensing over a wide range from 257 to 347 K. Owing to the unique temperature S of liquid crystal, different sensitivities and resolutions are observed at 257 to 297 K and 307 to 347 K, with S of 1.015 and 0.686 K/nm, and corresponding Q and FOM of 21.57 and 12.576, 0.076 K−1 and 0.003 04 K−1, respectively.

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Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising

Wu,

Brandt,

Zhou

et al. 2023

Biomed. Opt. Express

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We report on a compact multimodal imaging system that can acquire two-photon microscopy (2PM) and three-photon microscopy (3PM) images simultaneously. With dual excitation wavelengths, multiple contrasts including two-photon-excitation-fluorescence (2PEF), second harmonic generation (SHG), and third harmonic generation (THG) are acquired simultaneously from cells, collagen fibers, and interfaces, all label-free. Challenges related to the excitation by two wavelengths and the effective separation of 2PM and 3PM signals are discussed and addressed. The data processing challenge where multiple contrasts can have significantly varying signal levels is also addressed. A kernel-based nonlinear scaling (KNS) denoising method is introduced to reduce noise from ultra-low signal images and generate high-quality multimodal images. Simultaneous 2PM and 3PM imaging is demonstrated on various tissue samples. The simultaneous acquisition speeds up the imaging process and minimizes the commonly encountered problem of motion artifacts and mechanical drift in sequential acquisition. Multimodal imaging with simultaneous 2PM and 3PM will have great potential for label-free in-vivo imaging of biological tissues.

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Harmonic Generation and Impact of Phase Matching in Multimodal Multiphoton Microscopy

Cited by 3 publications

References 43 publications

Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Layered heterogeneous structures integrated device for multiplication, division arithmetic unit and multiple-physical sensing

Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising

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