Applications of polyion films containing biomolecules to sensing toxicity

Rusling, James F.; Zhou, Liping; Munge, Bernard; Yang, Jing; Estavillo, Carmelita; Schenkman, John B.

doi:10.1039/b001077o

Cited by 38 publications

(35 citation statements)

References 17 publications

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“…This value is comparable to previously published data for the Fe 2 + /Fe 3 + redox couple of heme thiolate proteins [22][23][24][25][26] and microperoxidase-11 (Table 1).…”

Section: Direct Electron Transfer Of Mp-11 Modified Goldsupporting

confidence: 92%

Bioelectrocatalysis by Microperoxidase‐11 in a Multilayer Architecture of Chitosan Embedded Gold Nanoparticles

et al. 2011

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We report on the redox behaviour of the microperoxidase-11 (MP-11) which has been electrostatically immobilized in a matrix of chitosan-embedded gold nanoparticles on the surface of a glassy carbon electrode. MP-11 contains a covalently bound heme c as the redox active group that exchanges electrons with the electrode via the gold nanoparticles. Electroactive surface concentration of MP-11 at high scan rate is between 350 AE 50 pmol cm À2, which reflects a multilayer process. The formal potential (E8') of MP-11 in the gold nanoparticles-chitosan film was estimated to be À(267.7AE2.9) mV at pH 7.0. The heterogeneous electron transfer rate constant (k s ) starts at 1.21 s À1 and levels off at 6.45 s À1 in the scan rate range from 0.1 to 2.0 V s À1. Oxidation and reduction of MP-11 by hydrogen peroxide and superoxide, respectively have been coupled to the direct electron transfer of MP-11.

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“…This value is comparable to previously published data for the Fe 2 + /Fe 3 + redox couple of heme thiolate proteins [22][23][24][25][26] and microperoxidase-11 (Table 1).…”

Section: Direct Electron Transfer Of Mp-11 Modified Goldsupporting

confidence: 92%

Bioelectrocatalysis by Microperoxidase‐11 in a Multilayer Architecture of Chitosan Embedded Gold Nanoparticles

et al. 2011

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“…Incubation of DNA/Mb films was done in buffer pH 5.5, which was previously shown to be the optimum for DNA damage by styrene oxide [41] and gave good turnover rates for styrene epoxidation [42]. Hydrogen peroxide was used at concentrations that do not damage DNA in the films [34] to activate myoglobin to the active ferryloxy radical form that transfers oxygen to the olefinic double bond of styrene.…”

Section: Influence Of Antioxidants On Dna Damage From Styrene Oxidementioning

confidence: 99%

Studies of DNA Damage Inhibition by Dietary Antioxidants Using Metallopolyion/DNA Sensors

Rusling

2006

Electroanalysis

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Voltammetric sensors featuring thin-films containing osmium and ruthenium metallopolymers were evaluated to monitor the influence of antioxidants on DNA damage reactions. In the first example, apigenin, chrysin, and ascorbic acid were shown to inhibit oxidation of DNA by hydroxyl radicals generated with Fentons reagent. A second example involved films of DNA with myoglobin (Mb) as an enzyme mimic which was used to convert styrene to styrene oxide. Here, the Ru peak of the sensor served as a marker of DNA damage from adduct formation between nucleobases and styrene oxide. There was no influence of the antioxidants on the reaction of styrene oxide itself with DNA films, but significant damage protection was afforded by micromolar amounts of antioxidants when styrene oxide was generated by Mb in the sensor films. This suggests that protection ensued from action of the antioxidants at the enzyme conversion level, probably by reduction of the active ferryloxy intermediate of the protein. Results demonstrate the usefulness of the thin metallopolyion/DNA film sensors in investigations of DNA damage inhibition.

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“…63,64 The Mb-DNA membrane would be useful for monitoring pollutants, as well as in the electrochemical detection of DNA damage. 65 Moreover, poly(ethylene oxide)-modified Mb was prepared and adapted to applications in organic solutions 66 and at high temperature.…”

Section: ·2 Myoglobinmentioning

confidence: 99%

Third-Generation Biosensors Based on the Direct Electron Transfer of Proteins

Zhang

2004

ANAL. SCI.

176

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Recent progress in third-generation electrochemical biosensors based on the direct electron transfer of proteins is reviewed. The development of three generations of electrochemical biosensors is also simply addressed. Special attention is paid to protein-film voltammetry, which is a powerful way to obtain the direct electron transfer of proteins. Research activities on various kinds of biosensors are discussed according to the proteins (enzymes) used in the specific work.

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Applications of polyion films containing biomolecules to sensing toxicity

Cited by 38 publications

References 17 publications

Bioelectrocatalysis by Microperoxidase‐11 in a Multilayer Architecture of Chitosan Embedded Gold Nanoparticles

Bioelectrocatalysis by Microperoxidase‐11 in a Multilayer Architecture of Chitosan Embedded Gold Nanoparticles

Studies of DNA Damage Inhibition by Dietary Antioxidants Using Metallopolyion/DNA Sensors

Third-Generation Biosensors Based on the Direct Electron Transfer of Proteins

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