Applications of two-dimensional thin-layer chromatography

Quantitative two-dimensional thin-layer chromatography using a diode-array detector

“…The same area of interest measured at different wavelengths in absorption (1)(2)(3)(4)(5) and fluorescence (6) shows that this method can improve the spot resolution Aalen, Germany) was used with an reflection attachment of 9 identical optical fibers ( Figure 3.3-1) with a diameter of 100 µm each (produced by TransMITCentre for Fibre Optics and Industrial Laser Applications, Gießen, Germany).…”

“…Since that time, this method has been mostly used for the separation of a large number of compounds that cannot be separated in a single dimension TLC experiment. In the first review on this topic G. Guiochon reported numerous applications of this technique [3]. The greatest dispersion of spots over the TLC plate is obtained when the chromatogram is developed in two orthogonal modes.…”

Milestones, Core Concepts, and Contrasts

“…If a single stationary phase is employed for the separation then the separation mechanisms differ only in the choice of mobile phase [28,37,46]. Separations with a reasonable increase in spot capacity still require that the separations in the orthogonal directions employ largely different retention mechanisms.…”

Contour diagrams for the presentation of separation quality in two‐dimensional thin layer chromatography

“…A recent review [2] illustrates that it is difficult to reach this potential even though relatively complex mixtures can be separated. In general, a large amount of trial and error is involved in selecting a suitable solvent for each of the two developments.…”