Applying genomic resources to accelerate wheat biofortification

Abstract

Wheat has low levels of the micronutrients iron and zinc in the grain, which contributes to 2 billion people suffering from micronutrient deficiency globally. While wheat flour is commonly fortified during processing, an attractive and more sustainable solution is biofortification, which could improve micronutrient content in the human diet, without the sustainability issues and costs associated with conventional fortification. Although many studies have used quantitative trait loci mapping and genome-wide association to identify genetic loci to improve micronutrient contents, recent developments in genomics offer an opportunity to accelerate marker discovery and use gene-focussed approaches to engineer improved micronutrient content in wheat. The recent publication of a high-quality wheat genome sequence, alongside gene expression atlases, variation datasets and sequenced mutant populations, provides a foundation to identify genetic loci and genes controlling micronutrient content in wheat. We discuss how novel genomic resources can identify candidate genes for biofortification, integrating knowledge from other cereal crops, and how these genes can be tested using gene editing, transgenic and TILLING approaches. Finally, we highlight key challenges remaining to develop wheat cultivars with high levels of iron and zinc.

Genetic Gain for Grain Micronutrients and Their Association with Phenology in Historical Wheat Cultivars Released between 1911 and 2016 in Pakistan

“…Velu et al [4] concluded that dietary supplements and agronomic practices involving the use of Feand Zn-containing fertilizers can help address the nutrient deficiency problem. However, a sustainable and cost-effective approach to increasing essential mineral concentration is through genetic biofortification, which requires identification of cultivars with useful genetic variability for grain minerals and understanding of the physiological and genetic architecture of these minerals in wheat [6].…”

Assessment of Biofortification Approaches Used to Improve Micronutrient-Dense Plants That Are a Sustainable Solution to Combat Hidden Hunger

“…However, being polyploid and having a large genome are compelling factors in wheat breeding. Thus, without a genome sequence, it has been difficult to design molecular markers and map loci that regulate the desired trait (Ali and Borrill 2020). A wheat landrace Chinese Spring genome sequence (Ref-Seqv1.0) representing 94% of the whole wheat genome was published in 2018, and the high contiguity of this genome assembly was reported to be effective in genetic mapping of loci involved in Fe and Zn accumulation (Appels et al 2018).…”

A Review of Omics Technologies and Bioinformatics to Accelerate Improvement of Papaya Traits

“…One major way to overcome the constraints due to climate change and papaya diseases is by breeding for new and improved papaya that has been enhanced with desired traits, such as resistance to disease, resistance to abiotic stress, delayed ripening, and sweetness. Recent trends in crop improvements have shown the integration of omics approaches (i.e., genomics, transcriptomics, proteomics, and metabolomics) and bioinformatics in breeding programmes [16][17][18][19]. The use of omics and bioinformatic approaches in crop breeding helps to obtain a holistic understanding of the genetic and genomic bases of the crop, as well as to understand the molecular interaction among genes, proteins, and metabolites, especially regarding complex traits.…”