Applying the Quartz Crystal Microbalance Technique to Detect the Epithelial Cell Tight Junction Integrality of Caco-2 Cells

Abstract

This paper describes a method that utilizes the quartz crystal microbalance (QCM) technique to measure the tight junction of the Caco-2 cell. The Caco-2 cells are placed on the QCM surface for cell growth and tight junction measurements. We found that the QCM resonance frequency changes less when a better tight cell junction is formed. The quantity of QCM frequency change is less than 100 Hz as the tight junction cell integrality is completed up to 15 days. The QCM and traditional detection system transepithelial electrical resistance were similar as the cell growth affects the two systems over time.

Nanoparticle and Bioparticle Deposition Kinetics: Quartz Microbalance Measurements

“…Because of its numerous advantages, the QCM method is used in an increasing number of investigations for nanoparticles involving liposomes and vesicles [ 44 , 45 , 56 , 96 ], macroions [ 31 , 51 , 52 , 53 ], proteins [ 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ], viruses [ 56 , 66 , 67 , 68 , 69 , 70 ], bacteria [ 71 , 72 , 73 , 74 , 75 , 76 , 77 ] and living cells, comprising cancerous ones [ 48 , 78 , 79 , 80 , 81 , 82 ]. However, as mentioned above, a quantitative theoretical interpretation of QCM measurements for bioparticles is often not feasible because the frequency and the dissipation shifts depend on many inadequately controlled parameters.…”

“…Moreover, in contrast to the streaming potential methods, the QCM signal is not directly affected by the solute or the interface charge. Because of its numerous advantages and a deceptive simplicity of measurements, the QCM method is used in numerous investigations involving nanoparticles [ 3 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ], macroions [ 29 , 31 , 51 , 52 , 53 ] proteins [ 27 , 28 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ], viruses [ 66 , 67 , 68 , 69 , 70 ], bacteria [ 71 , 72 , 73 , 74 , 75 , 76 , 77 ], and living cells [ 78 , 79 ,…”

A Survey of the 2010 Quartz Crystal Microbalance Literature

“…A disposable biosensor utilising quartz crystal resonance was developed for the analysis of Madin Darby canine kidney cells in a parallel design to allow correlation between the sensor signal and the cell distribution on the sensor surface (Cama et al ., ). Caco‐2 cells were grown on the surface on the QCM and the sensor was used to monitor the formation of tight cell junctions in the living cells (Chou and Yan, ), with relevance to the study of intestinal membrane integrity.…”