Appraisal of In-Vitro Antimicrobial, Thrombolytic and Membrane Stabilizing Potentials of Chloroform Extract of Avicennia Marina Leaves and Barks: An Approach to Develop Novel Drug

Abstract

The present study was aimed to assess ethno medicinal value of the plant. We tried to oversee in-vitro antimicrobial, thrombolytic and membrane stabilizing potentials of chloroform extracts of Avicennia marina leaves and barks. A. marina is an ethno medicinal plant belongs to the family of Acanthaceae. Crude chloroform extracts of A. marina (leaves & barks) dose dependently used for in vitro antimicrobial, thrombolytic and membrane stability screening. Chloroform extracts of A. marina at higher concentration evolved moderate antimicrobial activities against Gram negative (-ve) bacteria and were resistant against Gram positive (+ve) bacteria during assessment through disc diffusion method. During analysis of thrombolytic activity, 10mg/ml concentration of the extracts most significantly showed 16.33±0.49% lysis of clot (p<0.001), where streptokinase was used as standard & distilled water was treated as negative control. Again, Acetyl salicylic acid was used as standard in membrane stabilizing study whereas chloroform extracts of A.marina leaves and barks dose dependently showed inhibition of hemolysisat 10mg/ml, which was found 18.84±0.99% and 26.24±1.04% respectively for both hypotonic solution and heat induced condition of hemolysis of erythrocyte membrane.