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Objective: Cold methanolic extract of Suaeda maritima have been considered to screen their biological properties such as analgesic, membrane stabilizing and thrombolytic activity. Although some investigations have previously been reported with this plant, the whole plant was not studied yet to date. In the present study we aimed to investigate in vivo and in vitro bioactivities of Suaeda maritima. Material and Methods:The pharmacological analgesic activity was studied by acetic acid induced writhing method in vivo in a mouse model. Membrane stabilizing activity was assessed by inhibition of haemolysis at hypotonic solution induced condition in vitro, and thrombolytic activity was evaluated by employing Streptokinase as standard in vitro. Results: Extract of Suaeda maritima at a dose of 500 mg/kg body weight showed most significant inhibition of writhing at a rate of 82.25% in mice. Among all the fractions, crude methanol extract (ME) showed maximum effect with a value of 61.01% inhibition of hemolysis at 10 mg/ml dose, when standard acetyl salicylic acid (0. 10 mg/mL) revealed 66.37% inhibition of hemolysis. When clots were treated with 100 µl, moderate clot lysis activity observed with each of the test sample, however highest effect was found at 10 mg/ml dose (49. 13%). Conclusion:The Suaeda maritima extract showed a remarkable analgesic effect on a mouse model and membrane stabilizing and trombolytic activities in vivo models. It may introduce a new dimension in human model in analgesic, membrane stabilizing and antitrombolytic activities.

Aim: The present study was commenced to investigate the methanolic extracts of Launaea sarmentosa (Willd.) and Bruguiera cylindrical (L.) for antibacterial properties. Launaea sarmentosa (Willd.) is a plant of Asteraceae family while Bruguiera cylindrical (L.) belongs to the family of Rhizophoraceae. Materials and Method:The study was performed by disc diffusion method. Results:Crude methanolic extracts of both Launaea sarmentosa (Willd.) and Bruguiera cylindrical (L.) at various concentration were used for antibacterial screening. The methanolic extracts of Launaea sarmentosa (Willd.) produced good antibacterial activities against gram negative (-ve) bacteria and were resistant against gram positive (+ve) bacteria whereas crude methanolic extracts of Bruguiera cylindrical (L.) were resistant against both gram positive (+ve) and gram negative (-ve) bacteria. Conclusion:Further work especially bioassay-guided fractionation may be avouched in order to isolate and characterize the antibacterial active constituents responsible for the antibacterial property.

The present study was commenced to judge ethnomedicinal worth of the plant. We tried to analyze in vitro thrombolytic and membrane stabilising activities of methanolic extracts of Avicennia marina. Crude methanolic extracts of A. marina leaves & barks at numerous conc. were used for in vitro thrombolytic and membrane stabilising activities. Just in case of thrombolytic study, it absolutely was dose dependently accumulated, wherever 10 mg/ml concentration most importantly showed 13.22% lysis of clot (p<0.001) by in vitro clot lysis assay technique. Streptokinase was used as standard & water was treated as negative management. Crude methanolic extracts of A. marina dose dependently increased in membrane stabilising study, whereas 10 mg/ml concentration most importantly showed 10.11% & 22.23% inhibition of haemolysis severally by both hypotonic solution and heat induced haemolysis of RBC membrane. Acetyl salicylic acid was used as standard in membrane stabilising study.

The present study was aimed to assess ethno medicinal value of the plant. We tried to oversee in-vitro antimicrobial, thrombolytic and membrane stabilizing potentials of chloroform extracts of Avicennia marina leaves and barks. A. marina is an ethno medicinal plant belongs to the family of Acanthaceae. Crude chloroform extracts of A. marina (leaves & barks) dose dependently used for in vitro antimicrobial, thrombolytic and membrane stability screening. Chloroform extracts of A. marina at higher concentration evolved moderate antimicrobial activities against Gram negative (-ve) bacteria and were resistant against Gram positive (+ve) bacteria during assessment through disc diffusion method. During analysis of thrombolytic activity, 10mg/ml concentration of the extracts most significantly showed 16.33±0.49% lysis of clot (p<0.001), where streptokinase was used as standard & distilled water was treated as negative control. Again, Acetyl salicylic acid was used as standard in membrane stabilizing study whereas chloroform extracts of A.marina leaves and barks dose dependently showed inhibition of hemolysisat 10mg/ml, which was found 18.84±0.99% and 26.24±1.04% respectively for both hypotonic solution and heat induced condition of hemolysis of erythrocyte membrane.