Approach for semi-automated measurement of fiber diameter in murine and canine skeletal muscle

Stevens, Courtney R.; Berenson, Josh; Sledziona, Michael; Moore, Timothy P.; Dong, Lynn; Cheetham, Jonathan

doi:10.1371/journal.pone.0243163

Cited by 12 publications

(6 citation statements)

References 25 publications

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“…Reasons for exclusion during testing were, among others, that some tools required manual processing steps, could only be used on images from specific devices, needed paid software, or were not suitable for our images of fibrin fibers (Supplementary Table I). This last category includes tools that for example looked for cross-sections of fibers 20 , needed multi-channel images 21,22 , or needed specific kinds of structures, such as cell bodies in neurons 12 .…”

Section: Resultsmentioning

confidence: 99%

A systematic review and comparison of automated tools for quantification of fibrous networks

Vries

Laan

Frey

et al. 2022

Preprint

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Fibrous networks are essential structural components of biological and engineered materials. Accordingly, many approaches have been developed to quantify their structural properties, which define their material properties. However, a comprehensive overview and comparison of methods is lacking. Therefore, we systematically searched for automated tools quantifying network characteristics in confocal, stimulated emission depletion (STED) or scanning electron microscopy (SEM) images and compared these tools by applying them to fibrin, a prototypical fibrous network in thrombi. Structural properties of fibrin such as fiber diameter and alignment are clinically relevant, since they influence the risk of thrombosis. Based on a systematic comparison of the automated tools with each other, manual measurements, and simulated networks, we provide guidance to choose appropriate tools for fibrous network quantification depending on imaging modality and structural parameter. These tools are often able to reliably measure relative changes in network characteristics, but absolute numbers should be interpreted with care.

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Section: Resultsmentioning

confidence: 99%

A systematic review and comparison of automated tools for quantification of fibrous networks

Vries

Laan

Frey

et al. 2022

Preprint

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“…In the current study, to evaluate the effect of human DEC cells on muscle morphology, we applied an established morphometric method of the minimal Feret’s fiber diameter measurements [ 6 , 52 ] to further test and confirm the efficacy of DEC therapy in the amelioration of morphological abnormalities in the mdx -affected muscles.…”

Section: Discussionmentioning

confidence: 99%

Amelioration of Morphological Pathology in Cardiac, Respiratory, and Skeletal Muscles Following Intraosseous Administration of Human Dystrophin Expressing Chimeric (DEC) Cells in Duchenne Muscular Dystrophy Model

Siemionow,

Budzynska,

Zalants

et al. 2024

Biomedicines

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Duchenne Muscular Dystrophy (DMD) is a lethal disease caused by mutation in the dystrophin gene. Currently there is no cure for DMD. We introduced a novel human Dystrophin Expressing Chimeric (DEC) cell therapy of myoblast origin and confirmed the safety and efficacy of DEC in the mdx mouse models of DMD. In this study, we assessed histological and morphological changes in the cardiac, diaphragm, and gastrocnemius muscles of the mdx/scid mice after the transplantation of human DEC therapy via the systemic-intraosseous route. The efficacy of different DEC doses was evaluated at 90 days (0.5 × 106 and 1 × 106 DEC cells) and 180 days (1 × 106 and 5 × 106 DEC cells) after administration. The evaluation of Hematoxylin & Eosin (H&E)-stained sectional slices of cardiac, diaphragm, and gastrocnemius muscles included assessment of muscle fiber size by minimal Feret’s diameter method using ImageJ software. The overall improvement in muscle morphology was observed in DMD-affected target muscles in both studies, as evidenced by a shift in fiber size distribution toward the wild type (WT) phenotype and by an increase in the mean Feret’s diameter compared to the vehicle-injected controls. These findings confirm the long-term efficacy of human DEC therapy in the improvement of overall morphological pathology in the muscles affected by DMD and introduce DEC as a novel therapeutic approach for DMD patients.

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“…The main requirements to these types of meat will be objectivity, reproductivity and fast results. Over the recent years, there has been a noticeable tendency toward the development of express methods for studying microstructural parameters, and also developing new tools that would allow efficient analysis of transversal sections of meat for the purposes of studies and diagnostics (Song et al, 2020;Stevens et al, 2020). This may likely promote changes in the approaches to choosing criteria for meat quality.…”

Section: Discussionmentioning

confidence: 99%

Morphological signs of myopathy in pork that show no drastic decrease in pH after slaughter

Семенова

Кузнецова

Насонова

et al. 2021

Regul. Mech. Biosyst.

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High pH value 45 minutes after slaughter (рН45) has so far been the most frequently used indicator to select pig carcasses with normal course of autolysis. However, in practice, this does not provide meat quality homogeneity. Therefore, carcasses with рН45 > 6.0 were examined for signs of myopathy, which are characteristic for PSE meat, using the histological method. To perform the study, we randomly selected 320 individuals for slaughter out of 1,059 individuals of mixed swine grown in the same conditions. After slaughter, we selected 18 fresh carcasses that demonstrated low рН45. The results of the examination of the muscular tissue (L. dorsi) samples revealed that pork varied in microstructural characteristics. Only 44% of the samples had no signs of myopathy: no contracture nodes and destructive changes in the muscle fibers were present. A total of 39% of the samples were identified to the muscular tissue with mildly expressed myopathy, 17% of the samples – to the muscular tissue with acute myopathy. Thus, among the carcasses with рН45 > 6.0, 56% of the carcasses had signs of mild and acute myopathy, which explains quality homogeneity of meat selected using this criterion. Statistical analysis of the results suggested that the increase in the diameter of the muscle fibers of glycolytic type was related to appearance of signs of mild and acute myopathy – “giant fibers”. Increase in the weight of animals is not a risk factor. The obtained results allowed us to conclude the necessity of developing new approaches to assessing meat quality immediately after the slaughter with the purpose of increasing efficiency in predicting technological properties of meat. Promising directions of developing quick methods in histology allow us to hope that such approaches may be based on the data on microstructure of fresh muscular tissue.

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Approach for semi-automated measurement of fiber diameter in murine and canine skeletal muscle

Cited by 12 publications

References 25 publications

A systematic review and comparison of automated tools for quantification of fibrous networks

A systematic review and comparison of automated tools for quantification of fibrous networks

Amelioration of Morphological Pathology in Cardiac, Respiratory, and Skeletal Muscles Following Intraosseous Administration of Human Dystrophin Expressing Chimeric (DEC) Cells in Duchenne Muscular Dystrophy Model

Morphological signs of myopathy in pork that show no drastic decrease in pH after slaughter

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