Approach for simultaneous cannabidiol isolation and pesticide removal from hemp extracts with liquid-liquid chromatography

Luca, Simon Vlad; Roehrer, Simon; Kleigrewe, Karin; Minceva, Mirjana

doi:10.1016/j.indcrop.2020.112726

Cited by 27 publications

(22 citation statements)

References 22 publications

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“…C18 is the stationary phase commonly used in LC (Table 2). The C18 columns employed in the analysis of pesticides in cannabis were Restek ARC-18, 20,24,34,41 Kinetex C18, 25,39 XDB-C18, 19 BEH-C18, 37 Zorbax Eclipse plus C18, 33 Poroshell 120 EC-C18 5 and XB-C18. 39 Other stationary phases were also used as can be observed in Table 2.…”

Section: Separation and Detectionmentioning

confidence: 99%

“…The C18 columns employed in the analysis of pesticides in cannabis were Restek ARC-18, 20,24,34,41 Kinetex C18, 25,39 XDB-C18, 19 BEH-C18, 37 Zorbax Eclipse plus C18, 33 Poroshell 120 EC-C18 5 and XB-C18. 39 Other stationary phases were also used as can be observed in Table 2. In relation to mobile phases, non-buffered and buffered solvent mixtures were utilized, using water in combination with several acids or salts as aqueous mobile phase, whereas acetonitrile or methanol were used as organic phase.…”

Section: Separation and Detectionmentioning

confidence: 99%

“…38 This wide range of recoveries is because of the differences in the physicochemical properties of the 96 pesticides, enabling the determination of 82 out of 96 compounds. The second one 39 Water pipe and coated blade spray were also employed. Water pipe was used to extract four pesticides from cannabis smoke with recoveries between 60% and 70% 40 (Table 2) and coated blade spray was used to extract 37 pesticides from cannabis oil with acceptable recoveries (75-120%) (Table 2).…”

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confidence: 99%

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Recent applications of chromatography for analysis of contaminants in cannabis products: a review

López‐Ruiz¹,

Marín-Sáez²,

Frenich³

2021

Pest Management Science

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In the last few years, the cultivation of cannabis has been increasing due to greater use in foods, recreational use, creams, oils, and other applications. Thus, analysis of contaminants (e.g. pesticides and mycotoxins) in cannabis products is necessary to ensure consumer safety. This review is focused on the analytical procedures, based on chromatographic techniques, used for the determination of contaminants in cannabis and related products, developed from 2015 to 2020. QuEChERS (acronym of quick, easy, cheap, effective, rugged and safe) was mainly used for the extraction of pesticides and other contaminants from cannabis because its versatility and capacity to extract a wide range of substances, and therefore, increasing the scope of the analysis. The most employed technique to determine pesticides and mycotoxins in cannabis products was liquid chromatography (LC) coupled to mass spectrometry (MS), although gas chromatography (GC) coupled to MS was also employed for the analysis of non-polar compounds, using triple quadrupole (QqQ) as mass analyzer. Nevertheless, new advances in cannabis analysis are also discussed, introducing techniques such as high-resolution mass spectrometry (HRMS), which allows for performing both targeted and untargeted (unknown and suspect) analyses.

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Section: Separation and Detectionmentioning

confidence: 99%

Section: Separation and Detectionmentioning

confidence: 99%

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confidence: 99%

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Recent applications of chromatography for analysis of contaminants in cannabis products: a review

López‐Ruiz¹,

Marín-Sáez²,

Frenich³

2021

Pest Management Science

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“…In this work, the software COSMOconfX (Version 4.0, COSMOlogic, Leverkusen, Germany) and COSMOthermX (Version: C30 Release 16.01., COSMOlogic, Leverkusen, Germany) was used. The approach used for the screening and selection of biphasic solvent systems for CCC/CPC separations is described in detail in the literature [21][22][23].…”

Section: Biphasic Solvent System Screening With Predictive Thermodynamic Modelmentioning

confidence: 99%

“…The scope of the study was to find a biphasic solvent system that allows the simultaneous separation of the major compounds in the chamomile distillate. The conductor-like screening model for real solvents (COSMO-RS) was used as suggested in previous works to screen biphasic solvent systems [20][21][22][23]. In the biphasic liquid system, the target compounds should have a partition coefficient (P i ) in, or close to, the preferred "sweet spot" range (0.4 < P i < 2.5), as well as a separation factor greater than 1.5.…”

Section: Screening and Selection Of The Biphasic Solvent System For Ccc/cpcmentioning

confidence: 99%

Isolation of sesquiterpenoids from Matricaria chamomilla by means of solvent assisted flavor evaporation and centrifugal partition chromatography

et al. 2021

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The (semi)volatile fraction of Matricaria chamomilla L., an annual herbal plant from the family of Asteraceae, contains high quantities of sesquiterpenes and sesquiterpenoids. A method was developed to achieve isolation and separation of these compounds, using a combination of solvent assisted flavor evaporation (SAFE) and solid support-free liquid-liquid chromatography. The biphasic liquid solvent system n-heptane/ethyl acetate/methanol/water, 5/2/5/2 v/v/v/v (Arizona S) was elaborated as a suitable solvent system for the simultaneous separation of the target compounds. The lab-scale liquid-liquid chromatography separation performed in a countercurrent chromatography (CCC) column was successfully transferred to a semi-preparative centrifugal partition chromatography (CPC) column, which enabled the isolation of artemisia ketone, artemisia alcohol, α-bisabolone oxide A, and (E)-en-yn-dicycloether. α-Bisabolol oxide A and (Z)-en-yn-dicycloether co-eluted, but were successfully separated by subsequent size-exclusion chromatography (SEC). Similarly, spathulenol and α-bisabolol oxide B were obtained as a mixture, and were separated by means of column chromatography using silica gel as stationary phase. The isolated compounds were characterized by means of nuclear magnetic resonance spectroscopy (NMR) and gas chromatography–mass spectrometry (GC-MS). Graphical abstract

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Modeling the liquid‐liquid chromatography separation of cannabinoids from hemp extracts

Luca,

Gerigk,

Arshad

et al. 2024

J of Separation Science

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The separation of cannabinoids from hemp materials is nowadays one of the most promising industrial applications of liquid‐liquid chromatography (LLC). Despite various experimental research efforts to purify cannabinoids, there are currently few works on process modeling. Thus, this study aimed to explore a straightforward approach to model the LLC separation of cannabinoids from two hemp extracts with different compositions. The feed materials were simplified to mixtures of preselected key components (i.e., cannabidiol, tetrahydrocannabinol, cannabigerol, and cannabinol). The elution profiles of cannabinoids were simulated using the equilibrium‐cell model with an empirical nonlinear correlation. The model parameters were derived from the elution profiles of single‐solute pulse injections. For the validation of the proposed approach, LLC separations with the two hemp extracts were performed in descending mode with the solvent system composed of hexane/methanol/water 10/8/2 (v/v/v). The injected sample concentrations were gradually increased from 5 to 100 mg/mL. The results showed that the approach could describe reasonably well the elution behavior of the cannabinoids, with deviations of only 1–2 min between simulated and experimental elution times. However, to improve the prediction accuracy, the model parameters can be refitted to the elution profiles of 3–4 systematically selected pulse injections with specific hemp extracts.

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Approach for simultaneous cannabidiol isolation and pesticide removal from hemp extracts with liquid-liquid chromatography

Cited by 27 publications

References 22 publications

Recent applications of chromatography for analysis of contaminants in cannabis products: a review

Recent applications of chromatography for analysis of contaminants in cannabis products: a review

Isolation of sesquiterpenoids from Matricaria chamomilla by means of solvent assisted flavor evaporation and centrifugal partition chromatography

Modeling the liquid‐liquid chromatography separation of cannabinoids from hemp extracts

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