Aptamer adaptive binding assessed by stilbene photoisomerization towards regenerating aptasensors

Zhou, Yubin; Wu, Yuanyuan; Pokholenko, Oleksandr; Grimsrud, Marissa; Sham, Yuk Y.; Papper, Vladislav; Marks, Robert S.; Steele, Terry W. J.

doi:10.1016/j.snb.2017.10.135

Cited by 24 publications

(18 citation statements)

References 61 publications

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“…However, the degree of infiltration of giant cells was reduced to none for PAMAM-g-diazirine possibly due to the germicidal ability of –NH 2 groups [ 35 , 39 ]. These results indicate that the tissue adhesive warrants further investigation towards vascular patches, drug depots, and hydrogel biosensor implants [ 17 , 18 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ].…”

Section: Resultsmentioning

confidence: 99%

On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity

et al. 2018

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Tissue adhesives based on polyamidoamine (PAMAM) dendrimer, grafted with UV-sensitive aryldiazirine (PAMAM-g-diazirine) are promising new candidates for light active adhesion on soft tissues. Diazirine carbene precursors form interfacial and intermolecular covalent crosslinks with tissues after UV light activation that requires no premixing or inclusion of free radical initiators. However, primary amines on the PAMAM dendrimer surface present a potential risk due to their cytotoxic and immunological effects. PAMAM-g-diazirine formulations with cationic pendant amines converted into neutral amide groups were evaluated. In vitro toxicity is reduced by an order of magnitude upon amine capping while retaining bioadhesive properties. The in vivo immunological response to PAMAM-g-diazirine formulations was found to be optimal in comparison to standard poly(lactic-co-glycolic acid) (PLGA) thin films.

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Section: Resultsmentioning

confidence: 99%

On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity

et al. 2018

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“…Besides these advantages, aptamers are able to change conformation upon adaptive binding with their targets, providing a basis to induce fluorescent signaling/quenching events. 238,[254][255][256][257] Sosic et al 243 developed a multiplex sandwich aptamer microarray (SAM) for the simultaneous detection of VEGF 165 and thrombin. In this system, the modified aptamers Vap7(12T)NH 2 and the 15-mer TBA1(12T)NH 2 were anchored on distinct spots on a microarray slide as capture layer for VEGF 165 and thrombin respectively, while…”

Section: Fluorescence-based Vegf Aptasensors and Nano-aptasensorsmentioning

confidence: 99%

“…Fluorescence is the emission of light arising from direct photoexcitation of the emitting species 253 and is one of the most exploited techniques in the construction of highly selective biosensors, because of its rapidity of detection, high sensitivity and favorable cost‐effectiveness ratios. Besides these advantages, aptamers are able to change conformation upon adaptive binding with their targets, providing a basis to induce fluorescent signaling/quenching events 238,254–257 …”

Section: Dna‐based Aptasensors For Vegf Detectionmentioning

confidence: 99%

Anti‐VEGF DNA‐based aptamers in cancer therapeutics and diagnostics

Riccardi

Napolitano

Platella

et al. 2020

Medicinal Research Reviews

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The vascular endothelial growth factor (VEGF) family and its receptors play fundamental roles not only in physiological but also in pathological angiogenesis, characteristic of cancer progression. Aiming at finding putative treatments for several malignancies, various small molecules, antibodies, or protein‐based drugs have been evaluated in vitro and in vivo as VEGF inhibitors, providing efficient agents approved for clinical use. Due to the high clinical importance of VEGF, also a great number of anti‐VEGF nucleic acid‐based aptamers—that is, oligonucleotides able to bind with high affinity and specificity a selected biological target—have been developed as promising agents in anticancer strategies. Notable research efforts have been made in optimization processes of the identified aptamers, searching for increased target affinity and/or bioactivity by exploring structural analogues of the lead compounds. This review is focused on recent studies devoted to the development of DNA‐based aptamers designed to target VEGF. Their therapeutic potential as well as their significance in the construction of highly selective biosensors is here discussed.

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“…Stilbene derivatives have also been used as scaffolds for the construction of various supramolecular architectures including crown‐ether‐based charge‐transfer complexes, photochemically controlled hydrogels, and cucurbit[6]uril‐based rotaxane . In addition, the ( E )/( Z ) isomerisation ability of stilbenedisulfonate has been employed in sensing systems, such as in conjugation with oligonucleotides . Further examples can be found in recent reviews…”

Section: Introductionmentioning

confidence: 99%

A Photochemical/Thermal Switch Based on 4,4′‐Bis(benzimidazolio)stilbene: Synthesis and Supramolecular Properties.

et al. 2020

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Stilbene derivatives are well‐recognised substructures of molecular switches based on photochemically and/or thermally induced (E)/(Z) isomerisation. We combined a stilbene motif with two benzimidazolium arms to prepare new sorts of supramolecular building blocks and examined their binding properties towards cucurbit[n]urils (n=7, 8) and cyclodextrins (β‐CD, γ‐CD) in water. Based on the 1H NMR data and molecular dynamics simulations, we found that two distinct complexes with different stoichiometry, i. e., guest@β‐CD and guest@β‐CD2, coexist in equilibrium in a water solution of the (Z)‐stilbene‐based guests. We also demonstrated that the bis(benzimidazolio)stilbene guests can be transformed from the (E) into the (Z) form via UV irradiation and back via thermal treatment in DMSO.

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Aptamer adaptive binding assessed by stilbene photoisomerization towards regenerating aptasensors

Cited by 24 publications

References 61 publications

On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity

On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity

Anti‐VEGF DNA‐based aptamers in cancer therapeutics and diagnostics

A Photochemical/Thermal Switch Based on 4,4′‐Bis(benzimidazolio)stilbene: Synthesis and Supramolecular Properties.

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