Aptamer-based biosensors

Song, Shiping; Wang, Lihua; Li, Jiang; Fan, Chunhai; Zhao, Jianlong

doi:10.1016/j.trac.2007.12.004

Cited by 1,248 publications

(815 citation statements)

References 66 publications

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“…3 While protein enzymes and antibodies are the traditional molecules of choice for making biosensors, aptamers are emerging as a promising alternative due to their small sizes, high stability (especially DNA aptamers), ease of modification and immobilization, and excellent target recognition properties. [4][5][6][7][8][9][10][11][12][13][14][15][16] Aptamers are single-stranded nucleic acids that can selectively bind to target molecules. [17][18][19] Because of their useful properties, aptamers have been widely used for constructing biosensors.…”

Section: Introductionmentioning

confidence: 99%

Flow Cytometry-Assisted Detection of Adenosine in Serum with an Immobilized Aptamer Sensor

Huang

Liu

2010

Anal. Chem.

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Aptamers are single-stranded nucleic acids that can selectively bind to essentially any molecule of choice. Because of their high stability, low cost, ease of modification and availability through selection, aptamers hold great promise in addressing key challenges in bioanalytical chemistry. In the past fifteen years many highly sensitive fluorescent aptamer sensors have been reported. However, few such sensors showed high performance in serum samples. Further challenges related to practical applications include detection in a very small sample volume and a low dependence of sensor performance on ionic strength.We report the immobilization of an aptamer sensor on a magnetic microparticle and the use of flow cytometry for detection. Flow cytometry allows the detection of individual particles in a capillary and can effectively reduce the light scattering effect of serum. Since DNA immobilization generated a highly negatively charged surface and caused an enrichment of counter ions, the sensor performance showed a lower salt dependence. The detection limits for adenosine are determined to be 178 and 167 M in buffer and in 30% serum, respectively. Finally, we demonstrated that the detection can be carried out in 10 L of 90% human blood serum.3

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Section: Introductionmentioning

confidence: 99%

Flow Cytometry-Assisted Detection of Adenosine in Serum with an Immobilized Aptamer Sensor

Huang

Liu

2010

Anal. Chem.

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“…A significant advantage of the use of aptamers compared to antibody probes is that this approach can be readily adapted to any aptamer/antibody or aptamer/aptamer sandwich assay combination with the number of examples of both continuing to rise in the literature. [45][46][47][48] Furthermore, by exploring different nanoparticle shapes we highlight the versatility of this approach and we also expect the use of different modified DNA sequences binding to the aptamer will expand this approach to other surface-based signal transduction mechanisms such as electrochemistry. 49 As well as developing a new amplification mechanism, it was 15 demonstrated that even further improvements in sensitivity could be achieved compared to previous efforts focusing on a single nanoparticle amplification step.…”

Section: Resultsmentioning

confidence: 99%

Dual Nanoparticle Amplified Surface Plasmon Resonance Detection of Thrombin at Subattomolar Concentrations

2014

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This version is available at https://strathprints.strath.ac.uk/50087/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the AbstractA novel dual nanoparticle amplification approach is introduced for the enhanced surface plasmon resonance (SPR) detection of a target protein at subattomolar concentrations.Thrombin was used as a model target protein as part of a sandwich assay involving an antithrombin (anti-Th) modified SPR chip surface and a thrombin specific DNA aptamer (Thaptamer) whose sequence also includes a polyadenine (A30) tail. Dual nanoparticle (NP) enhancement was achieved with the controlled hybridization adsorption of first polythymine-NP conjugates (T20-NPs) followed by polyadenine-NP's (A30-NPs). Two different nanoparticle shapes (nanorod and quasi-spherical) were explored resulting in four different NP pair combinations being directly compared. It was found that both the order and NP shape were important in optimizing the assay performance. The use of real-time SPR measurements to detect target concentrations as low as 0.1 aM is a ten-fold improvement compared to single NP-enhanced SPR detection methods.

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“…After 6-12 consecutive cycles, the final library is cloned and sequenced. 24,74 The in vitro selection of aptamers eliminates the need for in vivo immunization of animals, as in the case of antibody production. Additionally, aptamers can be chemically synthesized in an easy and reproducible manner once the sequence is obtained using SELEX.…”

Section: Aptamer-based Sensing Approachesmentioning

confidence: 99%

“…Generally, 6-12 consecutive cycles are performed for each target and the final library is cloned and sequenced. 24,74 To date, aptamers-based assays have been intensively explored and widely applied. Fritz et al described an ultraselective and label-free assay for DNA detection.…”

Section: Aptamer-based Sensing Approachesmentioning

confidence: 99%

A review on electronic bio-sensing approaches based on non-antibody recognition elements

Chen

Huang

Palaniappan³

et al. 2016

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Aptamer-based biosensors

Cited by 1,248 publications

References 66 publications

Flow Cytometry-Assisted Detection of Adenosine in Serum with an Immobilized Aptamer Sensor

Flow Cytometry-Assisted Detection of Adenosine in Serum with an Immobilized Aptamer Sensor

Dual Nanoparticle Amplified Surface Plasmon Resonance Detection of Thrombin at Subattomolar Concentrations

A review on electronic bio-sensing approaches based on non-antibody recognition elements

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