Aptamer-based cell imaging reagents capable of fluorescence switching

Jung, Yun Kyung; Woo, Min-Ah; Soh, H. Tom; Park, Hyun Gyu

doi:10.1039/c4cc03888f

Cited by 28 publications

(13 citation statements)

References 40 publications

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“…In addition to the above‐mentioned aptamer–nanomaterial systems, other types of aptamer‐based nanomaterials, such as liposome, silica nanomaterials, polymer nanomaterials, MOFs, dendrimers, micellar nanoparticles, gadolinium oxide nanoparticles, calcium carbonate nanostructure, polydopamine nanospheres, MoS 2 nanoplates, lipid‐based nanobubbles, dipeptide nanoparticles, aggregation‐induced emission organic dots, and MnO 2 nanosheet, have been engineered as promising candidates toward in vivo applications in diverse areas. Based on these studies, we summarize the representative reports focusing on aptamers integrated with other nanomaterials, as shown in Table 2 .…”

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

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equally exciting and perhaps more challenging field of application is toward their in vivo applications, including living animals and human bodies, in diverse areas, [2] such as sensing, drug delivery, medical imaging, and cancer therapy. However, most of these nanomaterials lack selectivity toward targets of interests. Therefore, to employ these nanomaterials for a wider range of in vivo applications, various molecular engineering approaches have been employed to obtained nanomaterials functionalized with biomolecules to enable selective targeting. [3][4][5] Among these biomolecules, functional nucleic acids, or FNAs, have shown the most promise.FNAs are DNA or RNA molecules that can interact with or bind to a specific analyte, resulting in a conformational change and/or a catalytic reaction. [6] As their names suggested, ribozymes and deoxyribozymes (called DNAzyme hereafter) can act as enzymes in the presence of cofactors to catalyze various reactions, including cleavage and ligation of RNA/DNA, and phosphorylation and peroxidation of DNA. On the other hand, riboswitches and DNA aptamers are antibody-like receptors that can bind target molecules with high affinity and selectivity. Furthermore, the binding abilities of riboswitches and aptamers can be combined with the enzymatic function of ribozymes or DNAzymes to form aptazymes so that the binding of targets can inhibit or enhance the catalytic activities. These nucleic acids, including DNAzymes, aptamers, and aptazymes, are collectively known as FNAs to emphasize their functions beyond the traditional genetic storage and transformations roles for DNA and RNA. Unlike DNA and RNA inside biological systems, FNAs are isolated via a combinatorial technique known as in vitro selection, or a process also known as systematic evolution of ligands by exponential enrichment (SELEX). The discovery of new functions of nucleic acids has not only resulted in major advances in the fields of molecular biology and biochemistry, but also revolutionized sensors designs for both in vitro and in vivo applications. [7,8] Over the past decade, numerous FNA-based nanomaterials have been developed. [9][10][11][12] Among these FNA-based nanosystems, the functions of FNAs can be categorized into two types: diagnostic function and therapeutic function. For the diagnostic function, the FNAs serve either as the biorecognition ligands for direct sensing and imaging of the Recent advances in nanotechnology and engineering have generated many nanomaterials with unique physical and chemical properties. Over the past decade, numerous nanomaterials are introduced into many research areas, such as sensors for environmental monitoring, food safety, point-ofcare diagnostics, and as transducers for solar energy transfer. Meanwhile, functional nucleic acids (FNAs), including nucleic acid enzymes, aptamers, and aptazymes, have attracted major attention from the biomedical community due to their unique target recognition and catalytic properties. Benefiting from the recent progress of molecular engine...

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Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

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“…Other targets that have been imaged or tracked by fluorescent aptamers include epithelial cell adhesion molecule (EpCAM) [40, 41], thrombin [42], or amyloid plaques [43]. EpCAM is overexpressed in most solid cancers and most of the EpCAM-based diagnostic, prognostic, and therapeutic strategies resort to the use of an anti-EpCAM antibody.…”

Section: Fluorescence Imaging With Aptamersmentioning

confidence: 99%

“…These AAPs usually displayed a quenched fluorescence in its free state and was able to undergo a conformational alteration upon binding to the target with an activated fluorescence. One good example for cellular application is a recently developed aptamer-conjugated polydiacetylene imaging probe that shows highly specific fluorescence switching upon binding to epithelial cancer cells that overexpress EpCAM on their surface [41]. Another interesting new study produced an activatable imaging probe by adoption of fluorescence-quenched complementary sequence for an aptamer, applicable as a turn-on agent for imaging of aptamers in bacteria [45].…”

Section: Fluorescence Imaging With Aptamersmentioning

confidence: 99%

Applications of Aptamers in Targeted Imaging: State of the Art

Dougherty¹,

Cai

Hong³

2015

CTMC

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Aptamers are single-stranded oligonucleotides with high affinity and specificity to the target molecules or cells, thus they can serve as an important category of molecular targeting ligand. Since their discove1y, aptamers have been rapidly translated into clinical practice. The strong target affinity/selectivity, cost-effectivity, chemical versatility and safety of aptamers are superior to traditional peptides- or proteins-based ligands which make them unique choices for molecular imaging. Therefore, aptamers are considered to be extremely useful to guide various imaging contrast agents to the target tissues or cells for optical, magnetic resonance, nuclear, computed tomography, ultra sound and multimodality imaging. This review aims to provide an overview of aptamers' advantages as targeting ligands and their application in targeted imaging. Further research in synthesis of new types of aptamers and their conjugation with new categories of contrast agents is required to develop clinically translatable aptamer-based imaging agents which will eventually result in improved patient care.

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“…7,20,21 However, this antibody-based method is usually labor intensive, complicated, expensive, time-consuming and even requires highly skilled personnel. To date, only a few novel strategies, including electrochemical biosensors 20 and uorescence biosensors, 17,22 have been developed for the sensitive determination of EpCAM. Among them, uorescence biosensors are particularly attractive due to their high sensitivity, easy readout, simplicity and the feasibility of quantication.…”

Section: Introductionmentioning

confidence: 99%

Enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided DNA recycling amplification strategy

et al. 2018

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Aptamer-based cell imaging reagents capable of fluorescence switching

Abstract: We describe an aptamer-conjugated polydiacetylene imaging probe (ACP) that shows highly specific fluorescence switching upon binding to epithelial cancer cells that overexpress the tumor biomarker protein EpCAM (epithelial cell adhesion molecule) on their surface.

Cited by 28 publications

References 40 publications

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Applications of Aptamers in Targeted Imaging: State of the Art

Enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided DNA recycling amplification strategy

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