Aptamer-based Colorimetrie Biosensing of Ochratoxin A in Fortified White Grape Wine Sample Using Unmodified Gold Nanopartieles

Yin, Xueting; Wang, Sai; Liu, Xiaoyun; He, Chenmeng; Tang, Yali; Li, Qimeng; Liu, Jiahui; Su, Haijia; Tan, Tianwei; Dong, Yiyang

doi:10.2116/analsci.33.659

Cited by 49 publications

(20 citation statements)

References 23 publications

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“…These steps add time, and this is one reason for the continued development of non-enzymatic labels and label-free devices in order to achieve portability of the devices [47,48]. These disadvantages can be also overcome with the use of aptamer nucleotide sequences and AuNPs labels that are able to discriminate between various toxin categories [42][43][44][45]. However, for the time being, such formats tend to require expensive, laboratory-based equipment that requires significant technical training to operate.…”

Section: Discussionmentioning

confidence: 99%

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Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Mavrikou

Kintzios

2018

Beverages

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Mycotoxins and haloanisoles are secondary metabolites produced under special conditions of temperature and humidity by fungi colonizing a variety of commodities from preharvest up to consumer use. Ochratoxin A and 2,4,6-trichloanisole are produced mainly by species of the genus Aspergillus and Penicillium. Ochratoxin A exhibits nephrotic effects and can, potentially, be associated with human carcinogenesis, whereas 2,4,6-trichloanisole is primarily responsible for cork taint in wines. This review provides an overview of recent advances in biosensor technology for the determination of the aforementioned compounds in wine, beer and other beverages, as well as cork stoppers, which help in establishing and carrying out proper product quality-management strategies. Such a detailed investigation of biosensor-based detection methods of these toxic compounds in beverages could lead to the provision of safe-to-consume products, and allow the prioritization of future research efforts.

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Section: Discussionmentioning

confidence: 99%

“…Another simple and sensitive aptamer-based colorimetric method for the detection of OTA by using AuNPs has been developed by Yin et al [43]. The biosensor used a 36-mer aptamer (5 -GATCGGGTGTGGGTGGCGTAAAGGGACATCGGACA-3 ) as bio-recognition element and unmodified AuNPs as probes.…”

Section: Biosensors For the Detection Of Ochratoxin A In Wine Beer Amentioning

confidence: 99%

Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Mavrikou

Kintzios

2018

Beverages

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“…The authors propose this method to perform a useful screening for routine use in OTA determination in wine since it is rapid (125 min) and overcome the disadvantages of the unstable and expensive ELISA-based methods. Yin et al [148] have recently designed a novel aptamer-based colorimetric bioassay to determine OTA in fortified white wine samples. Free aptamers are absorbed onto gold nanoparticles surfaces, but when aptamers recognize OTA, gold particles aggregate.…”

Section: Analytical Methods For Ota Determination In Winementioning

confidence: 99%

Wine Contamination with Ochratoxins: A Review

et al. 2018

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Ochratoxin A (OTA) is the main mycotoxin occurring in wine. This review article is focused on the distribution of this toxin and its producing-fungi in grape berries, as well as on the fate of OTA during winemaking procedures. Due to its toxic properties, OTA levels in wine are regulated in different countries; therefore, it is necessary to apply control and detoxification methods that are also discussed in this revision.

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“…Immunochemical methods, such as those based on enzyme‐linked immunosorbent assays (ELISA) and immuno‐sensors , are also commonly utilized for OTA presence determination. Colorimetric biosensing of OTA using gold nanoparticle has also been reported . Fluorescence polarization, with its simplicity, rapidity, cost‐effectiveness, and reliability, is a promising candidate as a screening technique to identify OTA contamination .…”

Section: Introductionmentioning

confidence: 99%

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

et al. 2018

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A rapid and simple electrochemical method was utilized for the determination of the presence of classic fungal metabolite ochratoxin A (OTA) based on differential pulse adsorptive voltammetry, using the carbon nanotube paste (CNTP) electrode. OTA showed a clear oxidation peak, and the CNTP electrode exhibited a more sensitive response to OTA oxidation than the glassy carbon, the plastic formed carbon, and the carbon paste electrodes. The addition of long‐chain cationic surfactants, such as benzethonium chloride, cetyltrimethylammonium bromide, and benzyldimethyltetradecylammonium chloride (zephiramine), enhanced the electrochemical response of OTA. Under optimized experimental conditions, the calibration plots are linear in the 5×10−6−5×10−5 M OTA concentration range, and the limit of detection is 1.1×10−6 M. In order to improve the selectivity for OTA determination, we attempted to combine this adsorptive stripping voltammetric technique with “sequestration electrochemistry,” based on the specific interaction occurring between bovine serum albumin (BSA) and OTA. The peak current of OTA oxidation decreased substantially as a result of OTA‐BSA binding. Furthermore, it was demonstrated that the application of this proposed method of hydrodynamic adsorptive voltammetry, using a micro‐droplet and a rotating disk electrode, improves the approach's sensitivity and reduces the time and sample volume required for the analysis.

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Aptamer-based Colorimetrie Biosensing of Ochratoxin A in Fortified White Grape Wine Sample Using Unmodified Gold Nanopartieles

Cited by 49 publications

References 23 publications

Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Wine Contamination with Ochratoxins: A Review

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

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