Aptamer-based detection of Salmonella enteritidis using double signal amplification by Klenow fragment and dual fluorescence

Liu, Keyi; Yan, Xiaobing; Mao, Biyao; Wang, Sheng; Deng, Le

doi:10.1007/s00604-015-1692-4

Cited by 31 publications

(6 citation statements)

References 28 publications

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“…Consequently, targeting molecules modification may provide more intimate interaction between the material and the bacteria surrounded by extracellular polymeric substances. Aptamer, or chemical antibody, has been defined as a single‐stranded nucleic acid or short peptides, which can recognize a variety of targets with high affinity and specificity . Despite of their diverse application, aptamers are mainly applied in biosensor and biomedicine owing to its similar properties to antibodies .…”

Section: Introductionmentioning

confidence: 99%

Combat biofilm by bacteriostatic aptamer‐functionalized graphene oxide

Mao

Cheng

Wang

et al. 2018

Biotech and App Biochem

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Biofilms are the main reason for a large number deaths and high health costs. Their better protection compared to planktonic form against conventional antibiotics leads to poor treatment efficiency. Nanoagent-targeted delivery is a promising avenue for disease therapeutic, but its application targeting biofilms has not been reported currently. The roles, if any, of aptamers acting as delivery carrier and targeting factor, the graphene oxide (GO), and GO modified with aptamers against biofilms were then systematically evaluated. Here, we successfully developed an aptamer-targeted GO strategy against biofilms. We investigated the efficacy of aptamer-GO conjugates by UV spectrophotometer, inverted microscopy, and atomic force microscopy; 93.5 ± 3.4% Salmonella typhimurium biofilms were inhibited and 84.6 ± 5.1% of biofilms were dispersed by a ST-3-GO conjugate. More importantly, this conjugate represented distinctively toxicity to S. typhimurium. Thus, this strategy significantly displays excellent antibiofilm properties and may serve as a long-term solution for biofilm control.

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Section: Introductionmentioning

confidence: 99%

Combat biofilm by bacteriostatic aptamer‐functionalized graphene oxide

Mao

Cheng

Wang

et al. 2018

Biotech and App Biochem

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“…Enzyme-assisted target recycling and signal amplification designs have attracted significant attention owing to their high sensitivity [ 134 ]. To date, different types of nucleases, such as polymerases [ 123 , 135 ], exonucleases [ 136 , 137 ], deoxyribonucleases [ 132 , 138 ], and endonucleases [ 139 , 140 ], have been frequently employed to construct aptasensors for many target assays. The different properties of these nucleases can trigger several rounds of target recycling by implementing their endonuclease reaction or polymerization reaction.…”

Section: Aptamer-based Biosensormentioning

confidence: 99%

“…By using KF-aided target recycling amplification and exploiting the synergistic effects of FAM and the DNA probe SYBR Green I, the fluorescence was significantly amplified and the sensitivity improved. S. enteritidis could be assayed in milk samples by this method, with a detection limit of 300 CFU/mL [ 135 ]. He et al reported an electrochemical aptasensor for cocaine detection based on a KF polymerase reaction that combines the aggregation of ferrocene-functionalized oligonucleotide.…”

Section: Aptamer-based Biosensormentioning

confidence: 99%

Aptamers used for biosensors and targeted therapy

Ning

2020

Biomedicine & Pharmacotherapy

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“…The realization of the high sensitivity of the fluorescence immunoassay technology mainly depends on the amplification of the fluorescence signal (Wu et al, 2020b). Currently, many methods have been proposed based on fluorescence signal amplification strategies, including rolling circle amplification (Zhang et al, 2018b;Xiao et al, 2020), silver nanocluster amplification (Zhang et al, 2021), polymerization-induced nanobeads (Wu et al, 2020b), and nucleic acid fragment amplification (Liu et al, 2016;Yu et al, 2019). However, few studies have been done on the amplification of fluorescent signals based on polymer and streptavidin-biotin systems.…”

Section: Introductionmentioning

confidence: 99%

A fluorescent biosensor based on quantum dot–labeled streptavidin and poly-l-lysine for the rapid detection of Salmonella in milk

Ding

Yue

et al. 2022

Journal of Dairy Science

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Salmonella, as a common foodborne pathogen in dairy products, poses a great threat to human health. We studied a new detection method based on quantum dots (QD). A fluorescent biosensor with multiple fluorescent signal amplification based on a streptavidin (SA) biotin system and the polyamino linear polymer poly-l-lysine (PLL) were established to detect Salmonella in milk. First, Salmonella was captured on a black 96-well plate with paired Salmonella mAb to form a double-antibody sandwich. Second, SA was immobilized on biotin-modified mAb by SA-biotin specific bond. Then, the biotinmodified polylysine (BT-PLL) was bound on SA and specifically bonded again through the SA-biotin system. Finally, water-soluble CdSe/ZnS QD-labeled SA was added to a black 96-well plate for covalent coupling with BT-PLL. The fluorescent signal was amplified in a dendritic manner by the layer-by-layer overlap of SA and biotin and the covalent coupling of biotinylated PLL. Under optimal conditions, the detection limit was 4.9 × 10 3 cfu/mL in PBS. The detection limit was 10 times better than that of the conventional sandwich ELISA. In addition, the proposed biosensor was well specific and could be used for detecting Salmonella in milk samples.

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Aptamer-based detection of Salmonella enteritidis using double signal amplification by Klenow fragment and dual fluorescence

Cited by 31 publications

References 28 publications

Combat biofilm by bacteriostatic aptamer‐functionalized graphene oxide

Combat biofilm by bacteriostatic aptamer‐functionalized graphene oxide

Aptamers used for biosensors and targeted therapy

A fluorescent biosensor based on quantum dot–labeled streptavidin and poly-l-lysine for the rapid detection of Salmonella in milk

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